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Secretory expression of recombinant small laccase genes in Gram-positive bacteria
BackgroundLaccases are multicopper enzymes that oxidize a wide range of aromatic and non-aromatic compounds in the presence of oxygen. The majority...
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Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei
Trichoderma reesei ( T. reesei ) is well-known for its excellent ability to secret a large quantity of cellulase. However, unlike the endogenous...
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Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction
Lipids produced by oleaginous yeasts are considered as sustainable sources for the production of biofuels and oleochemicals. The red yeast Rhodosporidi...
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High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris
Collagen, the highest content protein in the body, has irreplaceable biological functions, and it is widespread concerned in food, beauty, and...
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A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli
BackgroundAlthough Escherichia coli has been widely used for the expression of exogenous proteins, the secretory expression in this system is still...
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Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
BackgroundThe filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading...
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Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae
BackgroundXylose contained in lignocellulosic biomass is an attractive carbon substrate for economically viable conversion to bioethanol. Extensive...
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Cytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagataella phaffii
BackgroundHuman lysozyme (hLYZ) is a natural antibacterial protein with broad applications in food and pharmaceutical industries. Recombinant...
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Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation
Prochiral pyrmetazole can be asymmetrically oxidized into ( S )-omeprazole, a proton pump inhibitor that is used to treat gastroesophageal reflux, by...
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Enhanced Secretory Expression and Surface Display Level of Bombyx mori Acetylcholinesterase 2 by Pichia pastoris Based on Codon Optimization Strategy for Pesticides Setection
The cholinesterase-based spectrophotometric assay, also called enzyme inhibition method, is a good choice for rapid detection of organophosphate...
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Secretory production of an engineered cutinase in Bacillus subtilis for efficient biocatalytic depolymerization of polyethylene terephthalate
Polyethylene terephthalate (PET) waste has caused serious environmental pollution. Recently, PET depolymerization by enzymes with PET-depolymerizing...
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Construction and testing of Yarrowia lipolytica recombinant protein expression chassis cells based on the high-throughput screening and secretome
BackgroundIn the recombinant protein market with broad economic value, the rapid development of synthetic biology has made it necessary to construct...
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High yield expression and purification of Aspergillus flavus uricase cloned in Pichia pink™ expression system
In this research, for the first time, A. flavus uricase gene was cloned in pPink-UOX plasmid under strong alcohol oxidase promoter of Pichia pink ...
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Development of an auto-inducible expression system by nitrogen sources switching based on the nitrogen catabolite repression regulation
BackgroundThe construction of protein expression systems is mainly focused on carbon catabolite repression and quorum-sensing systems. However, each...
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Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii
BackgroundEukaryotes use distinct networks of biogenesis factors to synthesize, fold, monitor, traffic, and secrete proteins. During heterologous...
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Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions
BackgroundAblynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is...
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Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris
Pectin lyase (PMGL) is an industrially important enzyme with widespread applications in the food, paper, and textile industries, owing to its...
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A novel sucrose-inducible expression system and its application for production of biomass-degrading enzymes in Aspergillus niger
BackgroundFilamentous fungi are extensively exploited as important enzyme producers due to the superior secretory capability. However, the complexity...
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Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins
BackgroundKluyveromyces marxianus is a promising cell factory for producing bioethanol and that raised a demand for a high yield of heterologous...
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Paramylon extracted from Euglena gracilis EOD-1 augmented the expression of SIRT1
Euglena gracilis , a type of microalgae, contains several nutrients and accumulates paramylon, a β-1,3-glucan. In recent studies, paramylon has shown...
