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Engineering of a Substrate Affinity Reduced S-Adenosyl-methionine Synthetase as a Novel Biosensor for Growth-Coupling Selection of L-Methionine Overproducers
Biosensors are powerful tools for monitoring specific metabolites or controlling metabolic flux towards the products in a single cell, which play...
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Breeding of Saccharomyces cerevisiae with a High-Throughput Screening Strategy for Improvement of S-Adenosyl-L-Methionine Production
S-adenosyl-l-methionine (SAM), a vital physiologically active substance in living organisms, is produced by fermentation over Saccharomyces cerevisiae ...
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Improvement of S-adenosyl-L-methionine production in Saccharomyces cerevisiae by atmospheric and room temperature plasma-ultraviolet compound mutagenesis and droplet microfluidic adaptive evolution
To improve S-Adenosyl-L-methionine (a compound with important physiological functions, SAM) production, atmospheric and room temperature plasma and...
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Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-l-methionine
Komagataella phaffii , formerly Pichia pastoris ( P. pastoris ), is a promising methylotrophic yeast used in industry to produce recombinant protein and...
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Microbial rhodoquinone biosynthesis proceeds via an atypical RquA-catalyzed amino transfer from S-adenosyl-L-methionine to ubiquinone
Rhodoquinone (RQ) is a close analogue of ubiquinone (UQ) that confers diverse bacterial and eukaryotic taxa the ability to utilize fumarate as an...
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The multiple effects of REG1 deletion and SNF1 overexpression improved the production of S-adenosyl-l-methionine in Saccharomyces cerevisiae
BackgroundSaccharomyces cerevisiae is often used as a cell factory for the production of S-adenosyl- l -methionine (SAM) for diverse pharmaceutical...
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Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme
d -Amino acid residues, found in countless peptides and natural products including ribosomally synthesized and post-translationally modified peptides...
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High-Throughput Screening and Directed Evolution of Methionine Adenosyltransferase from Escherichia coli
S-adenosyl-L-methionine (SAM) is the active form of methionine, which participates in various metabolic reactions and plays a vital role. It is...
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The identification and characterization of a selected SAM-dependent methyltransferase ribozyme that is present in natural sequences
RNA methylations contribute to a wide range of cellular functions. Cellular RNAs are usually methylated by protein methyltransferases using S -adenosyl-...
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A SAM analogue-utilizing ribozyme for site-specific RNA alkylation in living cells
Post-transcriptional RNA modification methods are in high demand for site-specific RNA labelling and analysis of RNA functions. In vitro-selected...
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Tracking DOT1L methyltransferase activity by stable isotope labelling using a selective synthetic co-factor
Epigenetic processes influence health and disease through mechanisms which alter gene expression. In contrast to genetic changes which affect DNA...
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Biotransformation to synthesize the methylated derivatives of baicalein using engineered Escherichia coli
Oroxylin A and negletein are flavonoid compounds existing in plants, with excellent pharmacological activities such as anti-inflammatory,...
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Effects and Regulatory Mechanism of Exogenous H2O2 on Amino Acid Metabolism in Post-harvest Broccoli: An Integrated Analysis of Transcriptomic and Metabolomic
An integrated transcriptome and metabolome profiling was used to investigate how exogenous H 2 O 2 affects amino acid metabolism and its underlying...
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Structure–function relationships of radical SAM enzymes
Radical S -adenosyl- l -methionine (SAM) enzymes belong to a family of catalysts whose number of annotated sequences is still growing. Upon the...
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Engineering cascade biocatalysis in whole cells for syringic acid bioproduction
BackgroundSyringic acid (SA) is a high-value natural compound with diverse biological activities and wide applications, commonly found in fruits,...
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Structural basis for non-radical catalysis by TsrM, a radical SAM methylase
Tryptophan 2C methyltransferase (TsrM) methylates C2 of the indole ring of l -tryptophan during biosynthesis of the quinaldic acid moiety of...
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PhoP- and GlnR-mediated regulation of metK transcription and its impact upon S-adenosyl-methionine biosynthesis in Saccharopolyspora erythraea
BackgroundErythromycin A (Er A) has a broad antibacterial effect and is a source of erythromycin derivatives. Methylation of erythromycin C (Er C),...
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Whole-cell catalysis by surface display of fluorinase on Escherichia coli using N-terminal domain of ice nucleation protein
BackgroundFluorinases play a unique role in the production of fluorine-containing organic molecules by biological methods. Whole-cell catalysis is a...
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Quantification of Plasma S-adenosylmethionine and S-adenosylhomocysteine Using Liquid Chromatography-Electrospray-Tandem Mass Spectrometry
We describe a simple stable isotope dilution method for accurate determination of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in...