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Simulation and Computational Study of RING Domain Mutants of BRCA1 and Ube2k in AD/PD Pathophysiology
Lysine-based post-translational modification (PTM) such as acylation, acetylation, deamination, methylation, SUMOylation, and ubiquitination has...
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Activity-based profiling of cullin–RING E3 networks by conformation-specific probes
The cullin–RING ubiquitin ligase (CRL) network comprises over 300 unique complexes that switch from inactive to activated conformations upon...
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Bottom-Up Synthetic Biology Using Cell-Free Protein Synthesis
Technical advances in biotechnology have greatly accelerated the development of bottom-up synthetic biology. Unlike top-down approaches, bottom-up...
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E3 Ubiquitin Ligase RNF125 Suppresses Immune Escape in Head and Neck Squamous Cell Carcinoma by Regulating PD-L1 Expression
Head and neck squamous cell carcinoma (HNSCC) is a lethal malignancy. Given the essential roles of E3 ligases in cancer immunotherapies, this paper...
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Computational insights into ligand–induced G protein and β-arrestin signaling of the dopamine D1 receptor
The dopamine D1 receptor (D1R), is a class A G protein coupled-receptor (GPCR) which has been a promising drug target for psychiatric and...
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Enhancement of recombinant human IL-24 (rhIL-24) protein production from site-specific integrated engineered CHO cells by sodium butyrate treatment
Interleukin-24 (IL-24) has specific inhibitory effects on the proliferation of various tumor cells with almost no toxicity to normal cells. The...
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Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production
Performance variability is a common challenge in cell-free protein productionCell-free protein production and hinders a wider adoption of these...
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Protein Labeling and Structure Determination by NMR Spectroscopy
NMR spectroscopy has proven itself to be invaluable in probing the structures of proteins that are not amenable to crystallization. In addition, NMR...
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Messenger RNA Surveillance: Current Understanding, Regulatory Mechanisms, and Future Implications
Nonsense-mediated mRNA decay (NMD) is an evolutionarily conserved surveillance mechanism in eukaryotes primarily deployed to ensure RNA quality...
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Regulation of Cephalosporin Biosynthesis
The filamentous fungus Acremonium chrysogenum is the natural producer of the β-lactam antibiotic cephalosporin C and is as such used worldwide in...
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Regulation of Gene Expression by Synthetic DNA-Binding Ligands
During the past 20 years, polyamides have evolved from the natural product distamycin to a new class of programmable heterocyclic oligomers that bind...
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Regulation of Penicillin Biosynthesis in Filamentous Fungi
The β-lactam antibiotic penicillin is one of the mainly used antibiotics for the therapy of infectious diseases. It is produced as end product by...
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DNA Major Groove Binders: Triple Helix-Forming Oligonucleotides, Triple Helix-Specific DNA Ligands and Cleaving Agents
Nucleic acids are polymorphic macromolecules that can adopt a variety of single-, double- and multi-stranded conformations, which in turn may provide...
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Ubiquitin-specific proximity labeling for the identification of E3 ligase substrates
Protein ubiquitylation controls diverse processes within eukaryotic cells, including protein degradation, and is often dysregulated in disease....
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Evaluation of antifouling/biofouling ability of a novel MIL101(Cr)/PES composite membrane for acetate wastewater treatment in MBR application
Membrane bioreactors (MBRs) as biotechnology-based wastewater treatment processes have recently attracted lots of attentions. However, membrane...
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The Proteostasis of Thymic Stromal Cells in Health and Diseases
The thymus is the key immune organ for the development of T cells. Different populations of thymic stromal cells interact with T cells, thereby...
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Organoid screening reveals epigenetic vulnerabilities in human colorectal cancer
Precision oncology presumes an accurate prediction of drug response on the basis of the molecular profile of tumors. However, the extent to which...
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Inclusion Bodies: Formation and Utilisation
The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level production in bacterial hosts and simple optimisation...
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Simulation of MDM2 N-terminal domain conformational lability in the presence of imidazoline based inhibitors of MDM2-p53 protein–protein interaction
Targeting of MDM2-p53 protein–protein interaction is a current approach for the development of potent anticancer agents. The classical pharmacophore...
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Extracellular Communication in Bacteria
Populations of bacterial cells often coordinate their responses to changes in their local environmental conditions through “quorum sensing”, a...