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Establishment and evaluation of digital PCR methods for HER2 copy number variation in breast cancer
Accurate measurement of human epidermal growth factor receptor 2 ( HER2 ) copy number variation (CNV) is very important for guiding the tumor target...
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A Real-Time Quantitative PCR Protocol for the Quantification of Plasmid Copy Number in Lactococcus lactis
The determination of the number of plasmid copies in each cell of Lactococcus lactisLactococcus lactis (L. lactis) is critical for the control and...
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The copy number variation of DMBT1 gene effects body traits in two Chinese cattle breeds
Copy number variations (CNVs) belong to mutations in the genome level with loci in the region of genic or intergenic. It is through different effects...
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Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number
BackgroundSynechocystis sp. PCC 6803 provides a well-established reference point to cyanobacterial metabolic engineering as part of basic...
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Genome-wide selective sweep analysis of the high-altitude adaptability of yaks by using the copy number variant
The domestic yak ( Bos grunniens ) from the Qinghai–Tibet Plateau is an important animal model in high-altitude adaptation studies. Here, we performed...
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Optimizing multicopy chromosomal integration for stable high-performing strains
The copy number of genes in chromosomes can be modified by chromosomal integration to construct efficient microbial cell factories but the resulting...
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Multiplex Functional Characterization of Protein Variant Libraries in Mammalian Cells with Single-Copy Genomic Integration and High-Throughput DNA Sequencing
Sequencing-based, massively parallel genetic assays have enabled simultaneous characterization of the genotype–phenotype relationships for libraries...
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Comparative genome-wide characterization of salt responsive micro RNA and their targets through integrated small RNA and de novo transcriptome profiling in sugarcane and its wild relative Erianthus arundinaceus
Soil salinity and saline irrigation water are major constraints in sugarcane affecting the production of cane and sugar yield. To understand the...
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Production of Tagatose by Whole-cell Bioconversion from Fructose Using Corynebacterium glutamicum
Tagatose is a rarely occurring sugar found in food and sweet fruits. It is a potential sweetener with low calories but a sweetness similar to that of...
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Copy number determination of the gene for the human pancreatic polypeptide receptor NPY4R using read depth analysis and droplet digital PCR
BackgroundCopy number variation (CNV) plays an important role in human genetic diversity and has been associated with multiple complex disorders....
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High-level Expression of an Acidic and Thermostable Chitosanase in Pichia pastoris Using Multi-copy Expression Strains and High-cell-density Cultivation
Chitin is a linear homopolymer of acetylated β-(1,4)-linked glucosamine residues and among the most abundant polysaccharides in the world. Here, we...
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Improvement of recombinant L-Asparaginase production in Pichia pastoris
Pichia pastoris is a successful expression system that is frequently preferred in the secretion of proteins for both basic research and industrial...
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Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene
BackgroundThe methylotrophic yeast, Pichia pastoris has been widely used for the production of human therapeutics, but production of granulocyte...
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Sustainable and high-level microbial production of plant hemoglobin in Corynebacterium glutamicum
BackgroundPlant hemoglobin shows great potential as a food additive to circumvent the controversy of using animal materials. Microbial fermentation...
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Development of SARS-CoV-2 packaged RNA reference material for nucleic acid testing
Nucleic acid tests to detect the SARS-CoV-2 virus have been performed worldwide since the beginning of the COVID-19 pandemic. For the quality...
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Cell envelope and stress-responsive pathways underlie an evolved oleaginous Rhodotorula toruloides strain multi-stress tolerance
BackgroundThe red oleaginous yeast Rhodotorula toruloides is a promising cell factory to produce microbial oils and carotenoids from lignocellulosic...
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Quantification and mitigation of byproduct formation by low-glycerol-producing Saccharomyces cerevisiae strains containing Calvin-cycle enzymes
BackgroundAnaerobic Saccharomyces cerevisiae cultures require glycerol formation to re-oxidize NADH formed in biosynthetic processes. Introduction...
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A culture-free method for rapidly and accurately quantifying active SARS-CoV-2
Determining the quantity of active virus is the most important basis to judge the risk of virus infection, which usually relies on the virus median...
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Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei
The fungus Trichoderma reesei is a powerful host for secreted production of proteins. The promoter of cdna1 gene, which encodes a small basic protein...
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A supernumerary synthetic chromosome in Komagataella phaffii as a repository for extraneous genetic material
BackgroundKomagataella phaffii (Pichia pastoris ) is a methylotrophic commercially important non-conventional species of yeast that grows in a...
