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Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production
BackgroundPyruvate is a widely used value-added chemical which also serves as a hub of various metabolic pathways. The fastest-growing bacterium Vibri...
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Enhanced pyruvate metabolism in plastids by overexpression of putative plastidial pyruvate transporter in Phaeodactylum tricornutum
BackgroundThe development of microalgal strains for enhanced biomass and biofuel production has received increased attention. Moreover, strain...
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Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species
BackgroundFuels and chemicals derived from non-fossil sources are needed to lessen human impacts on the environment while providing a healthy and...
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Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt
BackgroundCyanobacteria are promising hosts for the production of various industrially important compounds such as succinate. This study focuses on...
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Recombinant protein production provoked accumulation of ATP, fructose-1,6-bisphosphate and pyruvate in E. coli K12 strain TG1
BackgroundRecently it was shown that production of recombinant proteins in E. coli BL21(DE3) using pET based expression vectors leads to metabolic...
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Metabolic Engineering Design Strategies for Increasing Carbon Fluxes Relevant for Biosynthesis in Cyanobacteria
Cyanobacteria are promising microbial cell factories for the direct production of biochemicals and biofuels from CO2. Through genetic and metabolic...
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Construction and modular implementation of the THETA cycle for synthetic CO2 fixation
Synthetic biology offers the opportunity to build solutions for improved capture and conversion of carbon dioxide (CO 2 ) that outcompete those evolved...
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Comparison of the effects of nitrogen-, sulfur- and combined nitrogen- and sulfur-deprivations on cell growth, lipid bodies and gene expressions in Chlamydomonas reinhardtii cc5373-sta6
BackgroundBiofuel research that aims to optimize growth conditions in microalgae is critically important. Chlamydomonas reinhardtii is a green...
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Metabolite profiling of plant growth promoting cyanobacteria—Anabaena laxa and Calothrix elenkinii, using untargeted metabolomics
The metabolite profiles of two plant growth promoting cyanobacteria— Anabaena laxa and Calothrix elenkinii , which serve as promising biofertilizers,...
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Production of succinate with two CO2 fixation reactions from fatty acids in Cupriavidus necator H16
BackgroundBiotransformation of CO 2 into high-value-added carbon-based products is a promising process for reducing greenhouse gas emissions. To...
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Integration of Aspergillus niger transcriptomic profile with metabolic model identifies potential targets to optimise citric acid production from lignocellulosic hydrolysate
BackgroundCitric acid is typically produced industrially by Aspergillus niger -mediated fermentation of a sucrose-based feedstock, such as molasses....
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Computational evidence for the importance of lysine carboxylation in the reaction catalyzed by carboxyl transferase domain of pyruvate carboxylase: a QM/MM study
Posttranslational modification is a critical process in the cellular regulation, an example of which is the carboxylation of lysine. Pyruvate...
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Enhanced Production of Malic Acid by Co-localization of Phosphoenolpyruvate Carboxylase and Malate Dehydrogenase Using Synthetic Protein Scaffold in Escherichia coli
To produce malic acid from non-oxidative pathway route in Escherichia coli using two key enzymes and synthetic scaffold complex. E. coli was...
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A cell-free self-replenishing CO2-fixing system
Biological CO 2 fixation is so far the most effective means for CO 2 reduction at scale and accounts for most of the CO 2 fixed on Earth. Through this...
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Microbial Production of Dicarboxylates: Succinic Acid, Malic Acid, and Itaconic Acid
To establish a bio-based economy, suitable biomass-derived platform chemicals have to be identified, which can be an alternative for their...
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A non-carboxylative route for the efficient synthesis of central metabolite malonyl-CoA and its derived products
Acetyl coenzyme A (CoA) carboxylation is the natural route for endogenous malonyl-CoA formation; however, this pathway presents slow kinetics, carbon...
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Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism
BackgroundHaematococcus pluvialis is the best source of natural astaxanthin, known as the king of antioxidants. H. pluvialis have four cell forms:...
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Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16
BackgroundIndustrial biomanufacturing of value-added products using CO 2 as a carbon source is considered more sustainable, cost-effective and...
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Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803
BackgroundCyanobacteria can be metabolically engineered to convert CO 2 to fuels and chemicals such as ethylene. A major challenge in such efforts is...
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Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803
BackgroundCyanobacteria are emerging as green cell factories for sustainable biofuel and chemical production, due to their photosynthetic ability to...
