Search
Search Results
-
Nanopore Sequencing Methods and Protocols
This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through...
-
The Current State of Nanopore Sequencing
Nanopore sensing is a disruptive, revolutionary way in which to sequence nucleic acids, including both native DNA and RNA molecules. First...
-
Detection of DNA Modification Using Nanopore Sequencers
DNA modification is a crucial factor of epigenetic modification and has vital functions for gene regulation and phenotype control. A profound...
-
A Novel Detection Method of Fe(II) Based on Nanopore Sensor
AbstractA novel Fe(II) detection method was developed on nanopore platform in this work. The incubation mixture of dumbbell DNA and Bleomycin (BLM)...
-
Solid-State Nanopore/Nanochannel Sensing of Single Entities
Solid-state nanopores/nanochannels, with their high stability, tunable geometry, and controllable surface chemistry, have recently become an...
-
Nanopore/Nanocavity-Based Structures as Surface-Enhanced Raman Spectroscopy (SERS) Platforms
By measuring the inelastic scattering of monochromatic light, Raman spectroscopy is a method used to examine vibrational, rotational, and other...
-
Microbial Genome Sequencing and Assembly Using Nanopore Sequencers
Microbial genomes are typically several million base pairs in length and are relatively easy to sequence and assemble into a single chromosome, given...
-
Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This...
-
A Nanopore Sequencing Course for Graduate School Curriculum
The high-throughput long-read sequencing has become affordable enough for any molecular biology lab to utilize genome sequencing in their research....
-
Genomic Epidemiological Analysis of Antimicrobial-Resistant Bacteria with Nanopore Sequencing
Antimicrobial-resistant (AMR) bacterial infections caused by clinically important bacteria, including ESKAPE pathogens (Enterococcus faecium,...
-
Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK System
With the development of nanopore sequencing technology, long reads of DNA sequences can now be determined rapidly from various samples. This protocol...
-
Haplotype Inference Using Long-Read Nanopore Sequencing: Application to GSTA1 Promoter
Recovering true haplotypes can have important clinical consequences. The laboratory process is difficult and is, therefore, most often done through...
-
RNA Modification Detection Using Nanopore Direct RNA Sequencing and nanoDoc2
RNA modifications regulate multiple aspects of cellular function including RNA splicing, translation, export, decay, stability, and phase separation....
-
Ultralow-Input Genome Library Preparation for Nanopore Sequencing with Droplet MDA
Genome sequencing of small species, such as those of meinofauna, can be challenging due to the extremely low input of genomic DNA. While nanopore...
-
Nanopore Direct RNA Sequencing of Monosome- and Polysome-Bound RNA
Polysome fractionation makes use of density gradients and ultracentrifugation to separate transcripts based on their specific number of bound...
-
Protein nanopore reveals the renin–angiotensin system crosstalk with single-amino-acid resolution
The discovery of crosstalk effects on the renin–angiotensin system (RAS) is limited by the lack of approaches to quantitatively monitor, in real...
-
Nanopore microscope identifies RNA isoforms with structural colours
Identifying RNA transcript isoforms requires intricate protocols that suffer from various enzymatic biases. Here we design three-dimensional...
-
Finding Rearrangements in Nanopore DNA Reads with LAST and dnarrange
Long-read DNA sequencing techniques such as nanopore are especially useful for characterizing complex sequence rearrangements, which occur in some...
-
Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens
Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable measure for taxonomic profiling of bacterial communities. This...