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Efficient control of dissolved and colloidal substances in raw papermaking whitewater by immobilized thermophilic esterase from a metagenomic library
This study aimed to investigate the versatility of thermophilic esterase for treating raw papermaking whitewater with organic solvents at high...
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Shotgun Metagenomic Approaches
The most recent advancements in the knowledge of sourdough ecologic meta-community come from the uncultured approach based on shotgun metagenomics...
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Functional metagenomic screening identifies an unexpected β-glucuronidase
The abundance of recorded protein sequence data stands in contrast to the small number of experimentally verified functional annotation. Here we...
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Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides
The modular nature of nonribosomal peptide biosynthesis has driven efforts to generate peptide analogs by substituting amino acid-specifying domains...
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Biochemical characterization of a family IV esterase with R-form enantioselectivity from a compost metagenomic library
A novel family IV esterase (hormone-sensitive lipase, HSL) gene, est15L, was isolated from a compost metagenomic library. Encoded Est15L comprised...
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An improved protocol for metagenomic DNA isolation from low microbial biomass alkaline hot-spring sediments and soil samples
High-quality, humic-acid-free pure DNA is a prerequisite for functional and sequence-based approaches of metagenomics. In the present investigation,...
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Identification and Characterization of a Novel Carboxylesterase Belonging to Family VIII with Promiscuous Acyltransferase Activity Toward Cyanidin-3-O-Glucoside from a Soil Metagenomic Library
An alkaline esterase, designated as EstXT1, was identified through functional screening from a metagenomic library. Sequence analysis revealed that...
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Inactivation of Cronobacter sakazakii in powdered infant formula with probiotics and metagenomic analysis
In the present study, proper manual for powdered infant formula with probiotics (PIF-P) to prevent the contamination of Cronobacter sakazakii was...
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Metagenomic mining of Indian river confluence reveal functional microbial community with lignocelluloytic potential
Microbial carbohydrate-active enzymes (CAZyme) can be harnessed for valorization of Lignocellulosic biomass (LCB) to value-added chemicals/products....
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Antibacterial Property and Metagenomic Analysis of Milk Kefir
Milk kefir fermentation has been used in households for generations. Consumption of milk kefir has been associated with various health benefits,...
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Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins
The ability of unevolved amino acid sequences to become biological catalysts was key to the emergence of life on Earth. However, billions of years of...
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Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach
BackgroundGoat rumen microbial communities are perceived as one of the most potential biochemical reservoirs of multi-functional enzymes, which are...
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Metagenomic and physicochemical analysis of Kombucha beverage produced from tea waste
Kombucha beverage produced through fermentation of sugared tea using bacteria and yeast has gained attention for its beneficial health benefits....
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Lactobacillus casei Zhang exerts anti-obesity effect to obese glut1 and gut-specific-glut1 knockout mice via gut microbiota modulation mediated different metagenomic pathways
PurposeObesity is a major risk factor for various metabolic diseases, including metabolic syndrome and type-2 diabetes. Glucose transporter 1 (GLUT1)...
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A Novel VIII Carboxylesterase with High Hydrolytic Activity Against Ampicillin from a Soil Metagenomic Library
A novel carboxylesterase gene, named dlfae4 , was discovered and sequenced from a soil metagenomic library. The dlfae4 gene was composed of 1017 base...
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Metagenomic profiling of tomato rhizosphere delineates the diverse nature of uncultured microbes as influenced by Bacillus velezensis VB7 and Trichoderma koningiopsis TK towards the suppression of root-knot nematode under field conditions
The plant–parasitic Root Knot Nematodes ( Meloidogyne spp.,) play a pivotal role to devastate vegetable crops across the globe. Considering the...
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Management of microbial enzymes for biofuels and biogas production by using metagenomic and genome editing approaches
Non-renewable fossil fuels such as bitumen, coal, natural gas, oil shale, and petroleum are depleting over the world owing to unrestricted...
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Comparative metagenomic discovery of the dynamic cellulose-degrading process from a synergistic cellulolytic microbiota
To reveal the dynamic process of cellulose biodegradation and explore more potential cellulases, a microbiota (FPDM) with cellulose-degrading ability...
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Carbon migration and metagenomic characteristics during anaerobic digestion of rice straw
BackgroundConsiderable interest has been expressed in the development of anaerobic digestion (AD) of straw to solve the environmental problems caused...
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Functional mining of novel terpene synthases from metagenomes
BackgroundTerpenes are one of the most diverse and abundant classes of natural biomolecules, collectively enabling a variety of therapeutic, energy,...
