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FastKnock: an efficient next-generation approach to identify all knockout strategies for strain optimization
Overproduction of desired native or nonnative biochemical(s) in (micro)organisms can be achieved through metabolic engineering. Appropriate rewiring...
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RNA-Seq Analysis Reveals Altered Expression of Cell Adhesion-Related Genes Following PZR Knockout in Lung Cancer Cells
Protein zero related (PZR) serves as a substrate and anchor protein for SHP-2, the product of the proto-oncogene PTPN11 that is frequently mutated in...
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Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile
BackgroundThe hydrolysis and transphosphatidylation of phospholipase D (PLD) play important roles in the interconversion of phospholipids (PLs),...
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Fecal Protein Analysis of Dusp6 Knockout C57BL/6J Mice by Metaproteomics
The research of obesity and gut microbiota has been carried out for years, yet the study process was in a slow pace for several challenges to...
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A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Gene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological...
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Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris
Gene knockout is a key technology in the development of cell factories and basic research alike. The methylotrophic yeast Pichia pastoris is...
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Influence of ku70 Gene Knockout on the Transformation Frequency of Penicillium verruculosum Mycelial Fungi
AbstractTo increase the frequency of homologous recombination (HR) during the transformation of the industrial Penicillium verruculosum 221-151...
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Altered prefrontal neurochemistry in the DJ-1 knockout mouse model of Parkinson’s disease: complementary semi-quantitative analyses with in vivo magnetic resonance spectroscopy and MALDI-MSI
In vivo proton magnetic resonance spectroscopy ( 1 H-MRS) and matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) are two...
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Establishing Gene Expression and Knockout Methods in Esteya vermicola CBS115803
Pine wilt disease, which is caused by the nematode Bursaphelenchus xylophilus , is one of the most destructive forest diseases worldwide. Esteya...
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The Effect of the TacA Knockout Transcription Factor on the cbhI Gene Transcription and Expression in the Filamentous Fungus Strain Penicillium verruculosum
AbstractThe tacA gene encoding the TacA repressor protein is cloned by the method for “walking” in uncloned DNA from the genomic DNA of the fungus Pen...
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Analyze Mouse Knockout Models of UPR Pathway Elements
Evidence from genetic studies in human and mice indicates that defective skeletal development is one of the major phenotypic outcomes for aberrant...
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Sequential lipidomic, metabolomic, and proteomic analyses of serum, liver, and heart tissue specimens from peroxisomal biogenesis factor 11α knockout mice
Peroxisomes are versatile single membrane-enclosed cytoplasmic organelles, involved in reactive oxygen species (ROS) and lipid metabolism and diverse...
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CRISPR/Cas9-mediated knockout of clinically relevant alloantigenes in human primary T cells
BackgroundThe ability of CRISPR/Cas9 to mutate any desired genomic locus is being increasingly explored in the emerging area of cancer immunotherapy....
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Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses
BackgroundSmall animals that show a deficiency in klotho exhibit extremely shortened life span with multiple aging-like phenotypes. However, limited...
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One-Step Genoty** Method in loxP-Based Conditional Knockout Mice Generated by CRISPR-Cas9 Technology
With the development of CRISPR-Cas9 gene editing and in vitro fertilization (IVF) technology, we can now easily construct genetically modified mouse...
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The adc1 knockout with proC overexpression in Synechocystis sp. PCC 6803 induces a diversion of acetyl-CoA to produce more polyhydroxybutyrate
BackgroundLack of nutrients, in particular nitrogen and phosphorus, has been known in the field to sense glutamate production via 2-oxoglutarate and...
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Lactobacillus casei Zhang exerts anti-obesity effect to obese glut1 and gut-specific-glut1 knockout mice via gut microbiota modulation mediated different metagenomic pathways
PurposeObesity is a major risk factor for various metabolic diseases, including metabolic syndrome and type-2 diabetes. Glucose transporter 1 (GLUT1)...
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Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter
Although antibodies have attracted attention as next-generation biopharmaceuticals, the costs of purifying the products and of arranging the...
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A Machine Learning Approach for Predicting Essentiality of Metabolic Genes
The identification of essential genes is a key challenge in systems and synthetic biology, particularly for engineering metabolic pathways that...
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Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis
Renal interstitial fibrosis is the final common pathway in the process of all kidney diseases, and it results in chronic kidney disease. CCN2 is an...