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Coculture and Immobilization of Cellulolytic Bacteria for Enhanced Glucose Isomerase Production from Wheat Straw
Coculture and whole-cell immobilization have myriad industrial applications for enhancing enzyme production. Using pretreated wheat straw as the sole...
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Structural basis of the Meinwald rearrangement catalysed by styrene oxide isomerase
Membrane-bound styrene oxide isomerase (SOI) catalyses the Meinwald rearrangement—a Lewis-acid-catalysed isomerization of an epoxide to a carbonyl...
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Mining and Characterization of Thermophilic Glucose Isomerase Based on Virtual Probe Technology
Fructose, which is produced by the isomerization of glucose isomerase, is a crucial precursor for the biosynthesis of rare sugars. In this study,...
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Characterization of a novel ribose-5-phosphate isomerase B from Curtobacterium flaccumfaciens ZXL1 for D-allose production
Enzymatic preparation of rare sugars as an alternative to traditional sweeteners is an effective strategy to achieve a low-calorie healthy diet....
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Enhancement of L-ribulose Production from L-ribose Through Modification of Ochrobactrum sp. CSL1 Ribose-5-phosphate Isomerase A
L-ribulose, a kind of high-value rare sugar, could be utilized to manufacture L-form sugars and antiviral drugs, generally produced from L-arabinose...
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A Novel Glucose-6-Phosphate Isomerase Exists in Chicken Breast Meat: A Selenium-Containing Enzyme that Should Be Re-recognized Through New Eyes
Glucose-6-phosphate isomerase (GPI) is a highly conserved glycolytic enzyme in nature, and less information was available for GPI from hens. In this...
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Characterization of a Novel Mannose Isomerase from Stenotrophomonas rhizophila and Identification of Its Possible Catalytic Residues
D-Mannose has great value in the treatment of chronic diseases. D-Mannose isomerase can catalyze the bioconversion of D-fructose to D-mannose....
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Characterization of a Recombinant l-rhamnose Isomerase from Paenibacillus baekrokdamisoli to Produce d-allose from d-allulose
A putative l -rhamnose isomerase ( l -RI) gene from Paenibacillus baekrokdamisoli was expressed as a recombinant enzyme in Escherichia coli BL21(DE3)...
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Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae
BackgroundXylose contained in lignocellulosic biomass is an attractive carbon substrate for economically viable conversion to bioethanol. Extensive...
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Immobilization of Sucrose Isomerase from Erwinia sp. with Graphene Oxide and Its Application in Synthesizing Isomaltulose
Sucrose isomerase (SIase) is a key enzyme used for the production of isomaltulose from sucrose. In this study, an SIase gene from Erwinia sp . Ejp617 (
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Using phosphoglucose isomerase-deficient (pgi1Δ) Saccharomyces cerevisiae to map the impact of sugar phosphate levels on d-glucose and d-xylose sensing
BackgroundDespite decades of engineering efforts, recombinant Saccharomyces cerevisiae are still less efficient at converting d -xylose sugar to...
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Biochemical Properties of a Novel d-Mannose Isomerase from Pseudomonas syringae for d-Mannose Production
d -Mannose isomerase can reversibly catalyze d -fructose to d -mannose which has various beneficial effects. A novel d -mannose isomerase gene ( PsMIaseA )...
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Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum
BackgroundSugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among...
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Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor
To improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as...
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Studies on Biological Production of Isomaltulose Using Sucrose Isomerase: Current Status and Future Perspectives
AbstractIsomaltulose, as a safe sucrose substitute, is widely used as a functional sweetener due to its promising properties, such as slower...
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Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris
BackgroundPhytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme...
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A computer-aided approach to identify novel Leishmania major protein disulfide isomerase inhibitors for treatment of leishmaniasis
Leishmaniasis is an infectious disease caused by parasites of the genus Leishmania and transmitted by the bite of a sand fly. To date, most available...
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Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase...
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Homology modeling and molecular dynamics based insights into Chalcone synthase and Chalcone isomerase in Phyllanthus emblica L.
Chalcone synthase (CHS) and chalcone isomerase (CHI) plays a major role in the biosynthesis of flavonoid in plants. In this study, we made extensive...
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Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose
BackgroundSchizochytrium sp. is a heterotrophic, oil-producing microorganism that can efficiently produce lipids. However, the industrial production...
