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Study of Effect of Incubation Time and Temperature on the Linear Polyacrylamide-Coating Performance for the Separation of Proteins by Capillary Zone Electrophoresis
The preparation of linear polyacrylamide (LPA)-coated capillary is a multistep, laborious process. In this study, five different LPA-coating...
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Concentration and composition of the protein corona as a function of incubation time and serum concentration: an automated approach to the protein corona
Nanoparticles in contact with proteins form a “corona” of proteins adsorbed on the nanoparticle surface. Subsequent biological responses are then...
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Incubation Temperature and Period During Denarase Treatment and Microfiltration Affect the Yield of Recombinant Adenoviral Vectors During Downstream Processing
Adenoviral vectors (AV) are commonly used as vaccine and gene therapy vehicles because of their ease of construction, ability to grow to high titers...
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Reduction of the levels of 5-hydroxymethylfurfural and advanced glycation end products in milk by the combination of high pressure and moderate heat pre-incubation
Traditional thermal processing often denatures protein, causing loss of active ingredients and nutrients in dairy products, even the formation of...
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Self-assembly of a photoluminescent metal-organic cage and its spontaneous aggregation in dilute solutions enabling time-dependent emission enhancement
Charged metal-organic cages generally produce aggregates with various morphologies and different properties through the multiple supramolecular...
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Study on the effects of various incubation conditions on aggregation of SOD1 variants: disulfide bond reduction and demetallation synergistically promote generation of non-amyloid amorphous aggregates from SOD1 mutants
Amyotrophic lateral sclerosis (ALS) is a progressive fetal neurodegenerative disease that affects neural cells in the brainstem and spinal cord,...
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Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor
Electrochemical sensors based on antibody-antigen recognition events are commonly used for the rapid, label-free, and sensitive detection of various...
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Distinct phases of cellular signaling revealed by time-resolved protein synthesis
The post-translational regulation of protein function is involved in most cellular processes. As such, synthetic biology tools that operate at this...
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Real-time monitoring of intracellular biochemical response in locally stretched single cell by a nanosensor
Mechanotransduction is the essential process that cells convert mechanical force into biochemical responses, and electrochemical sensor stands out...
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Construction of programmed time-released multifunctional hydrogel with antibacterial and anti-inflammatory properties for impaired wound healing
The successful reprogramming of impaired wound healing presents ongoing challenges due to the impaired tissue microenvironment caused by severe...
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Time-Resolved In Situ AFM Measurement of Growth Rates of Aβ40 Fibrils
We employ time-resolved in situ atomic force microcopy to monitor the growth of individual Aβ40 fibrils and thereby directly measure the fibril...
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Sorting living mesenchymal stem cells using a TWIST1 RNA-based probe depends on incubation time and uptake capacity
Bone marrow derived mesenchymal stromal cells (BMSCs) are multipotent progenitors of particular interest for cell-based tissue engineering therapies....
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Quantitative Real-Time PCR for the Detection of Allergenic Species in Foods
Food allergy is an increasing challenge to public health, with widespread global distribution. With no cure for this pathology, the food-allergic...
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Predicting the effects of degradation on viscoelastic relaxation time using model transient networks
In this study, we utilized tetra-armed polyethylene glycol (Tetra-PEG) slimes, which are model transient networks with well-controlled structures, to...
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Synthesis of Hydroxyapatite Coating on Acid-Etched Titanium via Hydrothermal Method for Biomedical Applications: Effect of Temperature, Time, and pH Factor
AbstractIn this study, hydroxyapaptite (HAp) has been successfully coated on acid-etched titanium substrate by hydrothermal method. The phase...
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Oxygen Plasma for Prevention of Biofilm Formation on Silicone Catheter Surfaces: Influence of Plasma Exposure Time
The most common bacterial species responsible for causing catheter-associated urinary tract infection is Escherichia Coli ( E. Coli ) . In the present...
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Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of GFAP in serum
Glial fibrillary acidic protein (GFAP) in serum has been shown as a biomarker of traumatic brain injury (TBI) which is a significant global public...
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Reversible lysine-targeted probes reveal residence time-based kinase selectivity
The expansion of the target landscape of covalent inhibitors requires the engagement of nucleophiles beyond cysteine. Although the conserved...
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Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of retinol-binding protein 4 in urine
Urine retinol-binding protein 4 (RBP4) has recently been reported as a novel earlier biomarker of chronic kidney disease (CKD) which is a global...
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Time-resolved crystallography of boric acid binding to the active site serine of the β-lactamase CTX-M-14 and subsequent 1,2-diol esterification
The emergence and spread of antibiotic resistance represent a growing threat to public health. Of particular concern is the appearance of...
