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Heterologous expression of Arabidopsis laccase2, laccase4 and peroxidase52 driven under develo** xylem specific promoter DX15 improves saccharification in populus
BackgroundSecondary cell wall holds considerable potential as it has gained immense momentum to replace the lignocellulosic feedstock into fuels....
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Tobacco Plant: A Novel and Promising Heterologous Bioreactor for the Production of Recombinant Bovine Chymosin
The natural source of chymosin, a key enzyme in the dairy industry, is insufficient for rapidly growing cheese industries. Large-scale production of...
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A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
To mimic the Escherichia coli T7 protein expression system, we developed a facile T7 promoter-based protein expression system in an industrial...
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Heterologous Production of Antimicrobial Peptides: Notes to Consider
Heavy and irresponsible use of antibiotics in the last century has put selection pressure on the microbes to evolve even faster and develop more...
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Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae
Microbial proteins are promising substitutes for animal- and plant-based proteins. S. cerevisiae , a generally recognized as safe (GRAS)...
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Heterologous overproduction of oviedomycin by refactoring biosynthetic gene cluster and metabolic engineering of host strain Streptomyces coelicolor
BackgroundOviedomycin is one among several polyketides known for their potential as anticancer agents. The biosynthetic gene cluster (BGC) for...
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Identification and characterization of a strong constitutive promoter stnYp for activating biosynthetic genes and producing natural products in streptomyces
BackgroundStreptomyces are well known for their potential to produce various pharmaceutically active compounds, the commercial development of which...
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A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus
BackgroundEnzymes from thermophiles are of great interest for research and bioengineering due to their stability and efficiency. Thermophilic...
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“Metabolic burden” explained: stress symptoms and its related responses induced by (over)expression of (heterologous) proteins in Escherichia coli
BackgroundEngineering bacterial strains to redirect the metabolism towards the production of a specific product has enabled the development of...
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Towards the rate limit of heterologous biotechnological reactions in recombinant cyanobacteria
BackgroundCyanobacteria have emerged as highly efficient organisms for the production of chemicals and biofuels. Yet, the productivity of the cell...
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Bioactivity profiling of the extremolyte ectoine as a promising protectant and its heterologous production
Ectoine is a compatible solutes that is diffusely dispersed in bacteria and archaea. It plays a significant role as protectant against various...
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Development of constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli and Agrobacterium tumefaciens
Broad host range (BHR) expression vector is a vital tool in molecular biology research and application. Currently, most of the plasmid vectors used...
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Bidirectional hybrid erythritol-inducible promoter for synthetic biology in Yarrowia lipolytica
BackgroundThe oleaginous yeast Yarrowia lipolytica is increasingly used as a chassis strain for generating bioproducts. Several hybrid promoters...
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Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei
The fungus Trichoderma reesei is a powerful host for secreted production of proteins. The promoter of cdna1 gene, which encodes a small basic protein...
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Engineering heterologous enzyme secretion in Yarrowia lipolytica
BackgroundEukaryotic cells are often preferred for the production of complex enzymes and biopharmaceuticals due to their ability to form...
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Engineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coli
BackgroundLipoxygenase (EC. 1.13.11.12, LOX) can catalyze the addition of oxygen into polyunsaturated fatty acids to produce hydroperoxides, which...
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Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910
Unspecific peroxygenases (UPOs) are glycosylated enzymes that provide an efficient method for oxyfunctionalizing a variety of substrates using only...
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Cloning and Heterologous Expression of Natural Products from Actinobacteria
Biosynthetic gene clusters (BGCs) are imperative in producing natural products in the microbial world which has been regarded as the most important...
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Heterologous production of 3-hydroxypropionic acid in Methylorubrum extorquens by introducing the mcr gene via a multi-round chromosomal integration system based on cre-lox71/lox66 and transposon
Background and aimReprogramming microorganisms to enhance the production of metabolites is a part of contemporary synthetic biology, which relies on...
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The development of a heterologous gene expression system in thermophilic fungus Thermoascus aurantiacus
Thermoascus aurantiacus is a thermophilic fungus that belongs to the ascomycetous class and has attracted increasing interest for its ability to...
