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Fine tuning the glycolytic flux ratio of EP-bifido pathway for mevalonate production by enhancing glucose-6-phosphate dehydrogenase (Zwf) and CRISPRi suppressing 6-phosphofructose kinase (PfkA) in Escherichia coli
BackgroundNatural glycolysis encounters the decarboxylation of glucose partial oxidation product pyruvate into acetyl-CoA, where one-third of the...
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Characterization and Application of an Aspartate Dehydrogenase from Achromobacter denitrificans
A novel gene encoding aspartate dehydrogenase (ASPDH) has been discovered in Achromobacter denitrificans . The product of this gene has a strict...
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Enhanced bacterial cellulose production in Komagataeibacter sucrofermentans: impact of different PQQ-dependent dehydrogenase knockouts and ethanol supplementation
BackgroundBacterial cellulose (BC) is a biocompatible material with unique mechanical properties, thus holding a significant industrial potential....
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Exploring class III cellobiose dehydrogenase: sequence analysis and optimized recombinant expression
BackgroundCellobiose dehydrogenase (CDH) is an extracellular fungal oxidoreductase with multiple functions in plant biomass degradation. Its primary...
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Formate Dehydrogenase: From NAD(P)H Regeneration to Targeting Pathogen Biofilms, Composing Highly Efficient Hybrid Biocatalysts and Atmospheric CO2 Fixation
AbstractNAD(P) + -dependent formate dehydrogenase (EC 1.2.1.2, FDH) catalyzes the simplest reaction from chemical and biological points of view,...
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Expression Levels and Activity of Rat Liver Lactate Dehydrogenase Isoenzymes in Alloxan Diabetes
Abstract —A significant decrease in the activity of liver lactate dehydrogenase (LDH, EC 1.1.1.27) associated with a decrease in the expression of the...
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Characterization and Application of a Novel Glucose Dehydrogenase with Excellent Organic Solvent Tolerance for Cofactor Regeneration in Carbonyl Reduction
An efficient cofactor regeneration system has been developed to provide a hydride source for the preparation of optically pure alcohols by carbonyl...
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Chemical Hypoxic Preconditioning Improves Survival and Proliferation of Mesenchymal Stem Cells
Increasing evidence has demonstrated that mesenchymal stem cells (MSCs) have been linked to tissue regeneration both in vitro and in vivo. However,...
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Engineering of the Substrate Pocket of α-ketoglutaric Semialdehyde Dehydrogenase for Improving the Activity toward 3-hydroxypropanal
3-Hydroxypropionic acid (3-HP) is a key building block for value-added chemicals. A biological route for synthesizing this molecule is two-step...
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Constitutive glucose dehydrogenase elevates intracellular NADPH levels and luciferase luminescence in Bacillus subtilis
BackgroundGenetic modifications in Bacillus subtilis have allowed the conversion of myo -inositol into scyllo -inositol, which is proposed as a...
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A combination fermentation strategy for simultaneously increasing cellular NADP(H) level, biomass, and enzymatic activity of glufosinate dehydrogenase in Escherichia coli
Oxidoreductase is one of the most important biocatalysts for the synthesis of various chiral compounds. However, their whole-cell activity is...
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The Impact of Rosmarinic Acid on the Oxidative Stress Metabolism: Evaluation Protocol Based on Glutathione-Dependent and -Independent Antioxidant Enzymes
Phytochemicals are plant-based bioactive molecules produced by plants for their regular metabolism and to protect them against pathogens....
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Compositional and Morphological Analysis of Salt Stress Tolerant Mannitol-1-phosphate Dehydrogenase (mtlD)-Transgenic Potato Plants
Undesired effects often occur in genetically modified (GM) plants, especially during metabolite engineering. Nevertheless, conducting a comparative...
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Vitamin combination promotes ex vivo expansion of NK-92 cells by reprogramming glucose metabolism
Robust ex vivo expansion of NK-92 cells is essential for clinical immunotherapy. The vitamin B group is critical for the expansion and function of...
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Asymmetric Hydrogenation of C = C Bonds in a SpinChem Reactor by Immobilized Old Yellow Enzyme and Glucose Dehydrogenase
The application of immobilized enzymes in pharmaceutical and bulk chemical production has been shown to be economically viable. We demonstrate the...
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Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
BackgroundNumerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a...
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Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase
3-Hydroxybutanone (Acetoin, AC) and 2,3-butanediol (BD) are two essential four-carbon platform compounds with numerous pharmaceutical and chemical...
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O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1
Oncogenic Kras-activated pancreatic ductal adenocarcinoma (PDAC) cells highly rely on an unconventional glutamine catabolic pathway to sustain cell...
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Development of Voltammetric Glucose-6-phosphate Biosensors Based on the Immobilization of Glucose-6-phosphate Dehydrogenase on Polypyrrole- and Chitosan-Coated Fe3O4 Nanoparticles/Polypyrrole Nanocomposite Films
Polypyrrole (PPy) and PPy-containing chitosan-coated Fe 3 O 4 have been electrochemically polymerized on pencil graphite electrodes (PGEs). After the...
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Enzymatic Characterization and Coenzyme Specificity Conversion of a Novel Dimeric Malate Dehydrogenase from Bacillus subtilis
Malate is an important material to various industrials and clinical applications. Bacillus subtilis is a widely used biocatalyst tool for chemical...
