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Enzymatic debranching is a key determinant of the xylan-degrading activity of family AA9 lytic polysaccharide monooxygenases
BackgroundPrevious studies have revealed that some Auxiliary Activity family 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) oxidize and degrade...
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Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing
BackgroundThe abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial...
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Hemicellulases and Auxiliary Activities for Biomass Hydrolysis
Hemicelluloses are heteropolymers present along with cellulose in all terrestrial plant cell walls. Lignocellulosic biomass consists of 20–30%...
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Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review
The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role...
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CAZyme prediction in ascomycetous yeast genomes guides discovery of novel xylanolytic species with diverse capacities for hemicellulose hydrolysis
BackgroundAscomycetous yeasts from the kingdom fungi inhabit every biome in nature. While filamentous fungi have been studied extensively regarding...
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Enzymatic production of 4-O-methyl d-glucaric acid from hardwood xylan
BackgroundDicarboxylic acids offer several applications in detergent builder and biopolymer fields. One of these acids, 4- O -methyl d -glucaric acid,...
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Insights into the capability of the lignocellulolytic enzymes of Penicillium parvum 4-14 to saccharify corn bran after alkaline hydrogen peroxide pretreatment
BackgroundCorn bran is a major agro-industrial byproduct from corn starch processing. It contains abundant arabinoxylan that can be converted into...
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Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans
BackgroundNowadays there is a strong trend towards a circular economy using lignocellulosic biowaste for the production of biofuels and other...
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Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain
Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like...
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Xylooligosaccharides (XOS)
Xylooligosaccharides (XOS) are saccharides composed of xylose molecules linked by β-(1–4) bonds. They are considered nondigestible oligosaccharides...
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Structural and functional analysis of the active cow rumen’s microbial community provides a catalogue of genes and microbes participating in the deconstruction of cardoon biomass
BackgroundRuminal microbial communities enriched on lignocellulosic biomass have shown considerable promise for the discovery of microorganisms and...
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Exploration of genomic and functional features of chitinolytic bacterium Streptomyces chilikensis RC1830, isolated from Chilika Lake, India
Streptomyces chilikensis RC1830 was previously isolated as a novel chitinolytic streptomycete from Chilika Lake, Odisha, India. The strain RC1830 is...
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Carbohydrate active enzymes (CAZy) regulate cellulolytic and pectinolytic enzymes in Colletotrichum falcatum causing red rot in sugarcane
Colletotrichum falcatum, an ascomycete pathogen causes red rot of sugarcane which is specialized to infect cane stalks. Cellulolytic and pectinolytic...
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Cell-based and cell-free biocatalysis for the production of d-glucaric acid
d -Glucaric acid (GA) is a value-added chemical produced from biomass, and has potential applications as a versatile platform chemical, food additive,...
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Xylooligosaccharides (XOS)
Xylooligosaccharides (XOS) are saccharides composed of xylose molecules linked by β-(1–4) bonds. They are considered nondigestible oligosaccharides...
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Xylan-breakdown apparatus of Clostridium thermocellum
Clostridium thermocellum has been studied as a model organism that is highly specialized in cellulose deconstruction, and in secretion of...
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Systematic review of publicly available non-Dikarya fungal proteomes for understanding their plant biomass-degrading and bioremediation potentials
In the last two decades, studies on plant biomass-degrading fungi have remarkably increased to understand and reveal the underlying molecular...
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Development of an oligosaccharide library to characterise the structural variation in glucuronoarabinoxylan in the cell walls of vegetative tissues in grasses
BackgroundGrass glucuronoarabinoxylan (GAX) substitutions can inhibit enzymatic degradation and are involved in the interaction of xylan with cell...
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Recombinant Laccase Production Optimization in Pichia pastoris by Response Surface Methodology and Its Application in the Biodegradation of Octyl Phenol and 4-Tert-Octylphenol
The response surface methodology was used for the optimization of different submerged fermentation conditions for the production of recombinant...
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A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans
BackgroundAcetylated 4- O -(methyl)glucuronoxylan (GX) is the main hemicellulose in deciduous hardwood, and comprises a β-(1→4)-linked xylopyranosyl...
