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Role of Histidine 310 in Amydetes vivianii firefly luciferase pH and metal sensitivities and improvement of its color tuning properties
Firefly luciferases emit yellow-green light and are pH-sensitive, changing the bioluminescence color to red in the presence of heavy metals, acidic...
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Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases
The bioluminescence system of luminescent beetles has extensive applications in biological imaging, protein labeling and drug screening. To explore...
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Cloning and molecular properties of a novel luciferase from the Brazilian Bicellonycha lividipennis (Lampyridae: Photurinae) firefly: comparison with other firefly luciferases
Several firefly luciferases eliciting light emission in the yellow-green range of the spectrum and with distinct kinetic properties have been already...
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Hybridization-based CpG methylation level detection using methyl-CpG-binding domain–fused luciferase
Hypermethylation of tumor-suppressor genes and global hypomethylation, which is related to methylation level at the retroelement, have been...
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The orange light emitting luciferase from the rare Euryopa clarindae adult railroadworm (Coleoptera:Phengodidae): structural/functional and evolutionary relationship with green and red emitting luciferases
Railroadworms luciferases emit the widest range of bioluminescence colors among beetles, ranging from green to red, being model enzymes to...
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Luciferase isozymes from the Brazilian Aspisoma lineatum (Lampyridae) firefly: origin of efficient pH-sensitive lantern luciferases from fat body pH-insensitive ancestors
Firefly luciferases usually emit green-yellow bioluminescence at physiological pH values. However, under acidic conditions, in the presence of heavy...
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Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism
Cell communication network factor 2 (CCN2), also known as connective tissue growth factor (CTGF), is protein inducible in response to TGFβ/Smad...
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Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes)
Firefly adult bioluminescence functions as signal communication between sexes. How sympatric sibling species with similar glow pattern recognize...
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Effect of pH on the secondary structure and thermostability of beetle luciferases: structural origin of pH-insensitivity
Beetle luciferases were classified into three functional groups: (1) pH-sensitive yellow–green-emitting (fireflies) which change the bioluminescence...
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Synthesis of bioluminescent gold nanoparticle–luciferase hybrid systems for technological applications
Bioluminescent gold nanoparticles (AuNPs) were synthesized in situ using dithiol-terminated polyethylene glycol (PEG(SH) 2 ) as reducer and stabilizing...
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Role of E270 in pH- and metal-sensitivities of firefly luciferases
Firefly luciferases display a typical change in bioluminescence color to red at acidic pH, high temperatures and in the presence of heavy metals....
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Influence of the C-terminal domain on the bioluminescence activity and color determination in green and red emitting beetle luciferases and luciferase-like enzyme
Beetle luciferases catalyze the bioluminescent oxidation of D-luciferin, producing bioluminescence colors ranging from green to red, using two...
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Analysis of the impact of pluronic acid on the thermal stability and infectivity of AAV6.2FF
BackgroundThe advancement of AAV vectors into clinical testing has accelerated rapidly over the past two decades. While many of the AAV vectors being...
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A highly efficient, thermostable and cadmium selective firefly luciferase suitable for ratiometric metal and pH biosensing and for sensitive ATP assays
Firefly luciferases have been widely used for bioanalytical purposes during the last 5 decades. They usually emit yellow-green bioluminescence and...
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Novel application of Macrolampis sp2 firefly luciferase for intracellular pH-biosensing in mammalian cells
Bioluminescence is widely used in biosensors. Firefly luciferase-based bioluminescent sensors are among the most popular ones. Firefly luciferases...
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Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity
BackgroundBiological nitrogen fixation converting atmospheric dinitrogen to ammonia is an important way to provide nitrogen for plants. Pseudomonas...
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A new enzymatic assay to quantify inorganic pyrophosphate in plasma
Inorganic pyrophosphate (PPi) is a crucial extracellular mineralization regulator. Low plasma PPi concentrations underlie the soft tissue...
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Emitter as an Intramolecular Probe in a Luciferase Active Center
AbstractThe distinctive features of firefly luciferase bioluminescence are complex changes in the shape of the spectra and λ max of bioluminescence...
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Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae
The expression of plant cysteine oxidase (PCO) enzyme in Saccharomyces cerevisiae enables the Arg/Cys N-degron pathway (Cys-NDP) for selective...
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Small-Molecule Phosphine Activation of Protein Function in Zebrafish Embryos with an Expanded Genetic Code
Conditional control of protein function in a living model organism is an important tool for studying the effects of that protein during development...