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Establishment of a new promoter trap** vector using 2A peptide
Promoter trap** is a powerful tool for discovering promoters and uses promoter trap** vectors. However, the traditional trap** vector allows...
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Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity
BackgroundBiological nitrogen fixation converting atmospheric dinitrogen to ammonia is an important way to provide nitrogen for plants. Pseudomonas...
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Function Enhancement of a Metabolic Module via Endogenous Promoter Replacement for Pseudomonas sp. JY-Q to Degrade Nicotine in Tobacco Waste Treatment
Nicotine-degrading Pseudomonas sp. JY-Q is a preferred strain utilized in reconstituted tobacco process for tobacco waste treatment. However, its...
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Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering
BackgroundThe Lactobacillaceae family comprises many species of great importance for the food and healthcare industries, with numerous strains...
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Establishment of Transcriptional Gene Silencing Targeting the Promoter Regions of GFP, PDS, and PSY Genes in Cotton using Virus-Induced Gene Silencing
Virus-induced gene silencing (VIGS) by deploying viral-based vectors such as tobacco rattle virus (TRV) is a homology-based gene silencing technique...
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Haplotype Inference Using Long-Read Nanopore Sequencing: Application to GSTA1 Promoter
Recovering true haplotypes can have important clinical consequences. The laboratory process is difficult and is, therefore, most often done through...
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Activity of Arabidopsis Rubisco small subunit promoter in various tissues of chickpea
The activity of a green tissue-specific promoter of the Rubisco small subunit gene from Arabidopsis ( AraSSU ) was studied using transgenic chickpea...
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Role of Endogenous and Dietary Antioxidants in Brain Disorders
Oxidative stress occurs due to increased generation of reactive oxygen species than the body’s ability for antioxidant defense and repair. The brain...
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Screening and evaluation of the strong endogenous promoters in Pichia pastoris
BackgroundDue to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous...
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Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Phaffii
BackgroundHeme-incorporating peroxygenases are responsible for electron transport in a multitude of organisms. Yet their application in biocatalysis...
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Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei
The fungus Trichoderma reesei is a powerful host for secreted production of proteins. The promoter of cdna1 gene, which encodes a small basic protein...
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The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii
BackgroundThe budding yeast Komagataella phaffii ( Pichia pastoris ) is widely employed to secrete proteins of academic and industrial interest. For...
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Heterologous expression of Arabidopsis laccase2, laccase4 and peroxidase52 driven under develo** xylem specific promoter DX15 improves saccharification in populus
BackgroundSecondary cell wall holds considerable potential as it has gained immense momentum to replace the lignocellulosic feedstock into fuels....
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Genome-wide analysis reveals Hsf1 maintains high transcript abundance of target genes controlled by strong constitutive promoter in Saccharomyces cerevisiae
BackgroundIn synthetic biology, the strength of promoter elements is the basis for precise regulation of target gene transcription levels, which in...
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CircTFF1 Promotes Proliferation, Migration and Invasion of Lung Cancer Cells by Facilitating Methylation of BCL6B Promoter via miR-29c-3p/DNMT3A Axis
Lung cancer exists as a major risk of cancer-related death worldwide. As a novel circular RNA (circRNA), circTFF1 has not been explored in lung...
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Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis by promoter engineering and translation initiation efficiency optimization
BackgroundA raw starch-degrading α-amylase from Pontibacillus sp. ZY (AmyZ1), previously screened by our laboratory, showed a promising application...
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LINC02532 by Mediating miR-541-3p/HMGA1 Axis Exerts a Tumor Promoter in Breast cancer
The newly discovered LINC02532 is abnormally expressed in a variety of cancers and promotes cancer progression. The research proposed to discover the...
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Creation of a Synthetic Ligand for Mitochondrial DNA Sequence Recognition and Promoter-Specific Transcription Suppression
Synthetic ligands capable of DNA sequence recognition in human mitochondria are in increasing demand because more and more studies have revealed the...
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Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
BackgroundThe filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading...
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A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
To mimic the Escherichia coli T7 protein expression system, we developed a facile T7 promoter-based protein expression system in an industrial...
