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CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion
BackgroundHalophiles possess several unique properties and have broad biotechnological applications including industrial biotechnology production. Halomonas...
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C(sp3)–C(sp3) bond formation through nitrogen deletion of secondary amines using O-diphenylphosphinylhydroxylamine
The nitrogen deletion of secondary amines has emerged as an effective strategy for direct molecular skeletal editing and carbon–carbon bond...
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Design Principles of a Novel Construct for HBB Gene-Editing and Investigation of Its Gene-Targeting Efficiency in HEK293 Cells
Beta-thalassemia is one of the most common monogenic inherited disorders worldwide caused by different mutations in the hemoglobin subunit beta ( HBB )...
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UTX deletion promotes M2 macrophage polarization by epigenetically regulating endothelial cell-macrophage crosstalk after spinal cord injury
Macrophages polarized to the M2 subtype after spinal cord injury (SCI) are beneficial for promoting neurological recovery. The crosstalk between...
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Improving Fructooligosaccharide Production via sacC Gene Deletion in Zymomonas mobilis: A Novel Approach for Enhanced Prebiotic Production
Fructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. Industrially, these compounds are...
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Atomic Force Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with Endothelial-Specific Deletion of CCN1
Vascular stiffness is an independent predictor of human vascular diseases and is linked to ischemia, diabetes, high blood pressure, hyperlipidemia,...
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CRISPR-Cas9-Mediated NPC1 Gene Deletion Enhances HEK 293 T Cell Adhesion by Regulating E-Cadherin
NPC1 gene encodes a transmembrane glycoprotein on the late endosome/lysosomal membrane. Its mutation leads to a rare and aggravated autosomal...
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New insights into the construction of wild-type Saba pig-derived Escherichia coli irp2 gene deletion strains
To construct wild-type E. coli irp2 gene deletion strains, CRISPR/Cas9 gene editing technology was used, and the difficulty and key points of gene...
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Minimal Cells and Genome Minimization: Top-Down and Bottom-Up Approaches to Construct Synthetic Cells
Bioproduction of high-value molecules has traditionally been carried out using engineered host cells. However, toxic molecules and membrane proteins...
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Increasing lipid yield in Yarrowia lipolytica through phosphoketolase and phosphotransacetylase expression in a phosphofructokinase deletion strain
BackgroundLipids are important precursors in the biofuel and oleochemical industries. Yarrowia lipolytica is among the most extensively studied...
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Discrimination of deletion to point cytokine mutants based on an array of cross-reactive receptors mimicking protein recognition by heparan sulfate
Differential sensing of proteins based on cross-reactive arrays and pattern recognition is a promising technique for the detection and identification...
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Screening novel genes by a comprehensive strategy to construct multiple stress-tolerant industrial Saccharomyces cerevisiae with prominent bioethanol production
BackgroundStrong multiple stress-tolerance is a desirable characteristic for Saccharomyces cerevisiae when different feedstocks are used for...
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Superior production of heavy pamamycin derivatives using a bkdR deletion mutant of Streptomyces albus J1074/R2
BackgroundPamamycins are macrodiolides of polyketide origin which form a family of differently large homologues with molecular weights between 579...
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CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome
Efficient targeted genome engineering of Komagataella phaffii requires balanced expression of Cas9 nuclease and a target-specific guide RNA (gRNA)....
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Development of the thermophilic fungus Myceliophthora thermophila into glucoamylase hyperproduction system via the metabolic engineering using improved AsCas12a variants
BackgroundGlucoamylase is an important enzyme for starch saccharification in the food and biofuel industries and mainly produced from mesophilic...
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A genetic toolbox to empower Paracoccus pantotrophus DSM 2944 as a metabolically versatile SynBio chassis
BackgroundTo contribute to the discovery of new microbial strains with metabolic and physiological robustness and develop them into successful...
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Enhanced synthesis of 3-hydroxypropionic acid by eliminating by-products using recombinant Escherichia coli as a whole cell biocatalyst
3-hydroxypropionic acid (3-HP) is a valuable platform chemical with high demand in the global market. Owing to its sustainability and environmental...
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Enhanced protein secretion in reduced genome strains of Streptomyces lividans
BackgroundS. lividans TK24 is a popular host for the production of small molecules and the secretion of heterologous protein. Within its large...
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CRISPR/Cas9-mediated editing of phytoene desaturase (PDS) gene in an important staple crop, potato
The gene editing using the CRISPR/Cas9 system has become an important biotechnological tool for studying gene function and improving crops. In this...
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Unravelling key enzymatic steps in C-ring cleavage during angucycline biosynthesis
Angucyclines are type II polyketide natural products, often characterized by unusual structural rearrangements through B- or C-ring cleavage of their...
