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Extraction and analysis of high-quality chloroplast DNA with reduced nuclear DNA for medicinal plants
BackgroundObtaining high-quality chloroplast genome sequences requires chloroplast DNA (cpDNA) samples that meet the sequencing requirements. The...
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Chloroplast transformation in new cultivars of tomato through particle bombardment
A protocol has been established for genetic transformation of the chloroplasts in two new cultivars of tomato ( Solanum lycopersicum L.) grown in...
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Carotenoid Pathway Engineering in Tobacco Chloroplast Using a Synthetic Operon
The carotenoid pathway in plants has been altered through metabolic engineering to enhance their nutritional value and generate keto-carotenoids,...
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Chloroplast Engineering: Fundamental Insights and Its Application in Amelioration of Environmental Stress
Chloroplasts are specialized organelle that are responsible for converting light energy to chemical energy, thereby driving the carbon dioxide...
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Chloroplast genome skimming of a potential agroforestry species Melia dubia. Cav and its comparative phylogenetic analysis with major Meliaceae members
Melia dubia Cav. is a fast-growing plywood species gaining popularity due to high economic returns. This study aimed to assemble and annotate the...
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Reference-based assembly of chloroplast genome from leaf transcriptome data of Pterocarpus santalinus
Chloroplast genome sequencing is an essential tool to understand genome evolution and phylogenetic relationship. The available methods for...
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Detection of Carica papaya Adulteration in Piper nigrum Using Chloroplast DNA Marker-Based PCR Assays
The spice made from the fruits of Piper nigrum (PN) has economic and medicinal importance. Due to the high demand, as well as export and trade, the...
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DNA Isolation from Cocoa-Derived Products and Cocoa Authentication by TaqMan Real-Time PCR
Cocoa (Theobroma cacao L.) is an international commodity used as an ingredient in the manufacturing of chocolate making its authentication a key...
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A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii
The availability of routine methods for the genetic engineering of the chloroplast genome of Chlamydomonas reinhardtii is allowing researchers to...
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Overexpression the BnLACS9 could increase the chlorophyll and oil content in Brassica napus
BackgroundChlorophyll is a very important pigment involved in photosynthesis, while plant acyl-CoA biosynthesis is derived from plastid-localized...
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Conservation Strategies for Aquilaria sinensis: Insights from DNA Barcoding and ISSR Markers
The evergreen tree species Aquilaria sinensis holds significant economic importance due to its specific medicinal values and increasing market...
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Principles of Basic and Advanced Techniques Used for the Purification of Enzymes of Industrial Importance
Recovery and purification are very critical and essential steps in the downstream processing (DSP) of industrially important enzymes and compounds....
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Identification and analysis of RNA editing sites in chloroplast transcripts of kenaf (Hibiscus cannabinus L.)
RNA editing is one of the post-transcriptional modification processes and can lead to changes in sequencing and functioning of corresponding proteins...
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Fluorescent carbon-dots enhance light harvesting and photosynthesis by overexpressing PsbP and PsiK genes
BackgroundFluorescent carbon-dots (CDs) with multifaceted advantages have provided hope for improvement of crop growth. Near infrared (NIR) CDs would...
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Chloroplast proteins involved in drought stress response in selected cultivars of common bean (Phaseolus vulgaris L.)
One of the major cell organelles, whose functions are affected during drought stress are chloroplasts. In this study, chloroplast proteome under...
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Botanical Origin Authenticity Control of Pine Pollen Food Products Using Multiplex Species-Specific PCR Method
Pine pollen has been widely recognized for its comprehensive nutritional properties and health benefits, but it has also been the target of...
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Intrinsically Disordered Kiwellin Protein-Like Effectors Target Plant Chloroplasts and are Extensively Present in Rust Fungi
The effector proteins produced by plant pathogens are one of the essential components of host–pathogen interaction. Despite being important, most of...
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Evaluation of genome size and phylogenetic relationships of the Saccharum complex species
“ Saccharum complex” is a hypothetical group of species, which is supposed to be involved in the origin of modern sugarcane, and displays large...
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Plastid Transformation in Sugar Beet: An Important Industrial Crop
Chloroplast biotechnology has assumed great importance in the past 20 years and, thanks to the numerous advantages as compared to conventional...
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Investigation of carbon and energy metabolic mechanism of mixotrophy in Chromochloris zofingiensis
BackgroundMixotrophy can confer a higher growth rate than the sum of photoautotrophy and heterotrophy in many microalgal species. Thus, it has been...
