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Development of a Highly Sensitive Biotin-Streptavidin Amplified Enzyme-Linked Immunosorbent Assay for Determination of Progesterone in Milk Samples
Agricultural product contamination by endocrine-disrupting compounds (EDCs) residues is an international public health issue and requires...
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Multi-aptamer–mediated hairpin allosteric and aptamer-assisted CRISPR system for detection of S. pneumoniae and S. aureus
A novel nucleic acid aptamer nanoprobes-mediated hairpin allosteric and aptamer-assisted CRISPR system for detection of Streptococcus pneumoniae and St...
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On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen
Sensitive quantification of protein biomarkers is highly desired for clinical diagnosis and treatment. Yet, unlike DNA/RNA which can be greatly...
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A ratiometric fluorescent nanoprobe for signal amplification monitoring of intracellular telomerase activity
Telomerase is considered a valuable diagnostic and prognostic cancer biomarker. Accurate and reliable detection of telomerase activity is of great...
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Poly(arylene ethynylene)s in Chemosensing and Biosensing
Poly(arylene ethynylene)s (PArEs) have been used in recent years as effective transducers for a variety of sensing purposes ranging from organic...
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MOrPH-PhD: A Phage Display System for the Functional Selection of Genetically Encoded Macrocyclic Peptides
Macrocyclic peptides represent promising scaffolds for targeting biomolecules with high affinity and selectivity, making methods for the...
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Comprehensive Interactome Map** of Nuclear Receptors Using Proximity Biotinylation
Nuclear receptors, including hormone receptors, perform their cellular activities by modulating their protein–protein interactions. They engage with...
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Labeling and Detection of Modified Nucleic Acids
The nucleic acids were modified by various chemical groups to regulate gene expression without alteration of primary sequence. Until now, there are...
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Trends in Development of Aptamer-Based Biosensor Technology for Detection of Bacteria
The contamination of food by bacterial pathogens represents a substantial hazard for human and animal health. Therefore, considerable effort is...
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Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples
A fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and...
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Nucleic Acid Conjugates for Biosensing: Design, Preparation, and Application
A nucleic acid conjugate is a hybrid molecule consisting of a nucleic acid and a linked molecule that confers a specific function. The first nucleic...
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Process and Bioprocess Analysis
GenerallyProcess analysis speaking, thereInstrument(s) are two typical types of processes before us, the intermittent and the continuous. The...
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Phage-encoded bismuth bicycles enable instant access to targeted bioactive peptides
Genetically encoded libraries play a crucial role in discovering structurally rigid, high-affinity macrocyclic peptide ligands for therapeutic...
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Nucleic Acid Conjugates for Biosensing – Design, Preparation, and Application
A nucleic acid conjugate is a hybrid molecule consisting of a nucleic acid and a linked molecule that confers a specific function. The first nucleic...
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Selection and Application of Aptamer Affinity for Protein Purification
Aptamers are affinity-based oligonucleotide ligands raised against a target molecule, which might be of proteic or other nature. Aptamers are...
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Obtaining Detailed Phage Transcriptomes Using ONT-Cappable-Seq
Detailed transcription maps of bacteriophages are not usually explored, limiting our understanding of molecular phage biology and restricting their...
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Biomaterials Based on DNA Conjugates and Assemblies
In this chapter, biomaterial development based on DNA-sugar and DNA-peptide conjugates and DNA assemblies is highlighted. The conjugation of sugar...
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Dual-probe ligation without PCR for fluorescent sandwich assay of EGFR nucleotide variants in magnetic gene capture platform
A simple, rapid, and highly efficient fluorescent detection technique without PCR through dual-probe ligation with the genetic capture of magnetic...
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Ligand Selection for Affinity Chromatography Using Phage Display
Phage display coupled with in vitro affinity selection to mimic evolutionary principles has propelled the discovery of specific binding peptides and...
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NT-proBNP detection with a one-step magnetic lateral flow channel assay
Point-of-care sensors targeting blood marker analysis must be designed to function with very small volumes since acquiring a blood sample through a...
