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Systematic identification of CAZymes and transcription factors in the hypercellulolytic fungus Penicillium funiculosum NCIM1228 involved in lignocellulosic biomass degradation
BackgroundPenicillium funiculosum NCIM1228 is a filamentous fungus that was identified in our laboratory to have high cellulolytic activity....
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The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa
BackgroundFilamentous fungi are efficient degraders of plant biomass and the primary producers of commercial cellulolytic enzymes. While the...
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The transformations of cellulose after concentrated sulfuric acid treatment and its impact on the enzymatic saccharification
BackgroundThe dense structure of cellulose lowers its reactivity and hinders its applications. Concentrated sulfuric acid is an ideal solvent to...
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Exploring the multi-level regulation of lignocellulases in the filamentous fungus Trichoderma guizhouense NJAU4742 from an omics perspective
BackgroundFilamentous fungi are highly efficient at deconstructing plant biomass by secreting a variety of enzymes, but the complex enzymatic...
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An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production
BackgroundThe CRISPR/Cas9 technology is being employed as a convenient tool for genetic engineering of the industrially important filamentous fungus T...
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Improved consolidated bioprocessing for itaconic acid production by simultaneous optimization of cellulase and metabolic pathway of Neurospora crassa
Lignocellulose was directly used in itaconic acid production by a model filamentous fungus Neurospora crassa . The promoters of two clock control...
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Detection and quantitation of cellulose II by Raman spectroscopy
In cellulose materials, the cellulose II allomorph is often present either exclusively or in conjunction with cellulose I, the natural cellulose....
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Using One-pot Fermentation Technology to Prepare Enzyme Cocktail to Sustainably Produce Low Molecular Weight Galactomannans from Sesbania cannabina Seeds
Enzymatic hydrolysis using β-mannanase and α-galactosidase is necessary to produce low molecular weight galactomannan (LMW-GM) from galactomannans...
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Enhancing enzymatic saccharification yields of cellulose at high solid loadings by combining different LPMO activities
BackgroundThe polysaccharides in lignocellulosic biomass hold potential for production of biofuels and biochemicals. However, achieving efficient...
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Direct quantification of the degree of polymerization of hydrolyzed cellulose by solid-state NMR spectroscopy
Reducing chain ends in various hydrolyzed cellulose samples were quantitatively analyzed by solid-state 13 C NMR. Cellulose di- and tetramers served...
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Heterologous expression and characterization of novel GH12 β-glucanase and AA10 lytic polysaccharide monooxygenase from Streptomyces megaspores and their synergistic action in cellulose saccharification
BackgroundThe combination of cellulase and lytic polysaccharide monooxygenase (LPMO) is known to boost enzymatic saccharification of cellulose....
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Small GTPase Rab7 is involved in stress adaptation to carbon starvation to ensure the induced cellulase biosynthesis in Trichoderma reesei
BackgroundThe saprophytic filamentous fungus Trichoderma reesei represents one of the most prolific cellulase producers. The bulk production of...
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High-level production of Aspergillus niger prolyl endopeptidase from agricultural residue and its application in beer brewing
BackgroundProlyl endopeptidase from Aspergillus niger (AN-PEP) is a prominent serine proteinase with various potential applications in the food and...
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Enzymatic debranching is a key determinant of the xylan-degrading activity of family AA9 lytic polysaccharide monooxygenases
BackgroundPrevious studies have revealed that some Auxiliary Activity family 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) oxidize and degrade...
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Cellulose solubility in ionic liquids and regeneration in water and hydrogen peroxide solution: a comparative examination of morphology and physicochemical properties
The main objective of this work is to present a comprehensive analysis of the morphology, topography, and thermal behavior of modified cellulose as a...
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A novel decrystallizing protein CxEXL22 from Arthrobotrys sp. CX1 capable of synergistically hydrolyzing cellulose with cellulases
A novel expansin-like protein (CxEXL22) has been identified and characterized from newly isolated Arthrobotrys sp. CX1 that can cause cellulose...
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Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility
BackgroundDuring the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and...
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Impact of cellulose properties on its behavior in torrefaction: commercial microcrystalline cellulose versus cotton linters and celluloses extracted from woody and agricultural biomass
Biomass composition on cellulose, hemicelluloses and lignin determinates its behavior in torrefaction (200–350 °C, default-oxygen atmosphere). Up to...
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Integrative transcriptome and proteome analyses of Trichoderma longibrachiatum LC and its cellulase hyper-producing mutants generated by heavy ion mutagenesis reveal the key genes involved in cellulolytic enzymes regulation
BackgroundThe major challenge of facing the efficient utilization of biomass is the high cost of cellulolytic enzyme, while the Trichoderma...
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Coculture with hemicellulose-fermenting microbes reverses inhibition of corn fiber solubilization by Clostridium thermocellum at elevated solids loadings
BackgroundThe cellulolytic thermophile Clostridium thermocellum is an important biocatalyst due to its ability to solubilize lignocellulosic...
