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Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans
BackgroundNowadays there is a strong trend towards a circular economy using lignocellulosic biowaste for the production of biofuels and other...
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Hemicellulases and Auxiliary Activities for Biomass Hydrolysis
Hemicelluloses are heteropolymers present along with cellulose in all terrestrial plant cell walls. Lignocellulosic biomass consists of 20–30%...
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Characterization of glycoside hydrolase family 11 xylanase from Streptomyces sp. strain J103; its synergetic effect with acetyl xylan esterase and enhancement of enzymatic hydrolysis of lignocellulosic biomass
BackgroundXylanase-containing enzyme cocktails are used on an industrial scale to convert xylan into value-added products, as they hydrolyse the...
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Intracellular removal of acetyl, feruloyl and p-coumaroyl decorations on arabinoxylo-oligosaccharides imported from lignocellulosic biomass degradation by Ruminiclostridium cellulolyticum
BackgroundXylans are polysaccharides that are naturally abundant in agricultural by-products, such as cereal brans and straws. Microbial degradation...
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Xylan-breakdown apparatus of Clostridium thermocellum
Clostridium thermocellum has been studied as a model organism that is highly specialized in cellulose deconstruction, and in secretion of...
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An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate
Polyethylene terephthalate (PET) is a commodity polymer known to globally contaminate marine and terrestrial environments. Today, around 80 bacterial...
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Methionine inducing carbohydrate esterase secretion of Trichoderma harzianum enhances the accessibility of substrate glycosidic bonds
BackgroundThe conversion of plant biomass into biochemicals is a promising way to alleviate energy shortage, which depends on efficient microbial...
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Control of phenotypic diversification based on serial cultivations on different carbon sources leads to improved bacterial xylanase production
Thermobacillus xylanilyticus is a thermophilic and hemicellulolytic bacterium of interest for the production of thermostable hemicellulases. Enzymes’...
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Characterization of an acetyl xylan esterase from the marine bacterium Ochrovirga pacifica and its synergism with xylanase on beechwood xylan
BackgroundAcetyl xylan esterase plays an important role in the complete enzymatic hydrolysis of lignocellulosic materials. It hydrolyzes the ester...
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Multimodular fused acetyl–feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass
BackgroundPlant biomass is an abundant and renewable carbon source that is recalcitrant towards both chemical and biochemical degradation. Xylan is...
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Exploring the multi-level regulation of lignocellulases in the filamentous fungus Trichoderma guizhouense NJAU4742 from an omics perspective
BackgroundFilamentous fungi are highly efficient at deconstructing plant biomass by secreting a variety of enzymes, but the complex enzymatic...
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High-level production of xylose from agricultural wastes using GH11 endo-xylanase and GH43 β-xylosidase from Bacillus sp.
As a promising feedstock, alkali-extracted xylan from lignocellulosic biomass is desired for producing xylose, which can be used for renewable...
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Microbial Conversion of Biomass
Microbial conversion of biomass is a natural process of transformation of biological resources into energy and bioproducts. Lignocellulosic and...
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Discovery of novel alkaline-tolerant xylanases from fecal microbiota of dairy cows
Xylo-oligosaccharides (XOS) are considered as a promising type of prebiotics that can be used in foods, feeds, and healthcare products. Xylanases...
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Dependence of Enzymatic Saccharification on the Type of Biomass and Pretreatment
Variety of agricultural crops and other forest residues form an important lignocellulosic biomass feedstock for biorefineries. However, these...
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Xylanolytic enzyme consortia from Bacillus sp. NIORKP76 for improved biobleaching of kraft pulp
A cellulase-free xylanolytic enzyme consortia consisting of a xylanase, arabinofuranosidase, and acetyl xylan esterase produced by Bacillus sp....
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Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing
BackgroundThe abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial...
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Functioning of a tripartite lignocellulolytic microbial consortium cultivated under two shaking conditions: a metatranscriptomic study
BackgroundIn a previous study, shaking speed was found to be an important factor affecting the population dynamics and lignocellulose-degrading...
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Xylooligosaccharides (XOS)
Xylooligosaccharides (XOS) are saccharides composed of xylose molecules linked by β-(1–4) bonds. They are considered nondigestible oligosaccharides...
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Microbial Conversion of Biomass
Microbial conversion of biomass is a natural process of transformation of biological resources into energy and bioproducts. Lignocellulosic and...