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Ribosomal RNA Recognition by Aminoglycoside Antibiotics
2-Deoxystreptamine (2-DOS) aminoglycosides are a family of structurally related broad-spectrum antibiotics that are used widely in the clinic. Their...
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Folding molecular origami from ribosomal RNA
Approximately 80 percent of the total RNA in cells is ribosomal RNA (rRNA), making it an abundant and inexpensive natural source of long,...
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Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens
Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable measure for taxonomic profiling of bacterial communities. This...
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Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK System
With the development of nanopore sequencing technology, long reads of DNA sequences can now be determined rapidly from various samples. This protocol...
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Linear-Time Algorithms for RNA Structure Prediction
RNA secondary structure prediction is widely used to understand RNA function. Existing dynamic programming-based algorithms, both the classical...
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RNA Modification Detection Using Nanopore Direct RNA Sequencing and nanoDoc2
RNA modifications regulate multiple aspects of cellular function including RNA splicing, translation, export, decay, stability, and phase separation....
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Functional Engineering of Synthetic RNA Through Circularization
Many types of RNAs exist in the cells, such as messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), noncoding RNA (ncRNA), and microRNA...
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Automated 3D Design and Evaluation of RNA Nanostructures with RNAMake
Despite growing interest in applying RNA’s unique structural characteristics to solve diverse biotechnology and nanotechnology problems, there are...
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16S rRNA Methyltransferases as Novel Drug Targets Against Tuberculosis
Tuberculosis (TB) is an airborne infectious disease caused by Mycobacterium tuberculosis ( M.tb ) whose natural history traces back to 70,000 years. TB...
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Study on the Bioconversion of Curcumin to Calebin-A Using Spirulina subsalsa and Its Taxonomic Resolution Using 16S rRNA Analysis
Due to morphological convergence and the application of numerous taxonomic concepts, the systematics of filamentous cyanobacteria is still a...
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Functional Engineering of Synthetic RNA Through Circularization
Many types of RNAs exist in the cells, such as messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), noncoding RNA (ncRNA), and microRNA...
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Structures, mechanisms and applications of RNA-centric CRISPR–Cas13
Prokaryotes are equipped with a variety of resistance strategies to survive frequent viral attacks or invading mobile genetic elements. Among these,...
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Comparative 16S Metabarcoding of Nile Tilapia Gut Microbiota from the Northern Lakes of Egypt
Nile tilapia, Oreochromis niloticus , is the principal fish bred in Egypt. A pilot study was designed to analyze the bacterial composition of the Nile...
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Naked-eye detection with loop-mediated isothermal amplification for P. carotovorum subsp. carotovorum in agricultural products
Pectobacterium carotovorum causing soft-rot disease requires on-site detection before the distribution of agricultural products. Loop-mediated...
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Complete mitogenome sequence analysis of an Indian Black Bengal goat, Capra hircus, and its phylogenetic consequences
Capra hircus (Black Bengal goat) is a prized goat breed reared across the Southeast Asian region. Known for its excellent chevon quality and...
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Three-dimensional structure-guided evolution of a ribosome with tethered subunits
RNA-based macromolecular machines, such as the ribosome, have functional parts reliant on structural interactions spanning sequence-distant regions....
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Diversity of Actinobacteria in Various Habitats
Actinobacteria are ubiquitous, diverse, and one of the biggest lineages in the domain Bacteria. Their existence has been documented in a variety of...
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Culture-Independent Estimation of Lactic Acid Bacteria and Yeasts
The population balances inside microbial consortia are highly variable from one sourdough ecosystem to another. In general, growth rate and yield of...
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Effects of Different Preparation Methods on Microbiota Composition of Fecal Suspension
Fecal microbiota transplantation is an emerging disease-modifying therapy. The viability of the microbiome in feces and its successful transfer...
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An innovative molecular approach towards the cost-effective entomological authentication of honey
Honey authentication and traceability are crucial not only for economic purposes but also for ensuring safety. However, the widespread adoption of...
