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Fusion of Oligopeptide to the C Terminus of α-Glucuronidase from Thermotoga maritima Improves the Catalytic Efficiency for Hemicellulose Biotransformation
Fusion protein combined the oligopeptide (HQAFFHA) with the C terminus of α-glucuronidase from Thermotoga maritima was produced in E. coli and...
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Trisubstituted Imidazole and N-Propargyl Imidazole Analogues: Synthesis, Characterization, In Silico Studies and Enzyme Inhibitory Properties
AbstractIn recent years, the α-amylase, pancreatic lipase (PL), and β-glucuronidase enzymes have received much attention as they promise to be...
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Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain
Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like...
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Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing
BackgroundThe abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial...
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Hemicellulases and Auxiliary Activities for Biomass Hydrolysis
Hemicelluloses are heteropolymers present along with cellulose in all terrestrial plant cell walls. Lignocellulosic biomass consists of 20–30%...
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Anti-adipogenic Effects of the Probiotic Lactiplantibacillus plantarum KU15117 on 3T3-L1 Adipocytes
In this study, we investigated the probiotic properties and anti-obesity effects of bacterial strains isolated from homemade kimchi. Lactiplantibacillu...
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A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes
BackgroundMicroalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the...
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Probiotic properties of novel probiotic Levilactobacillus brevis KU15147 isolated from radish kimchi and its antioxidant and immune-enhancing activities
This study was conducted to evaluate the probiotic properties and antioxidant activities of lactic acid bacteria strains including Levilactobacillus...
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A “dilute-and-shoot” column-switching UHPLC–MS/MS procedure for the rapid determination of branched nonylphenol in human urine: method optimisation and some fundamental aspects of nonylphenol analysis
Technical grade branched nonylphenol (NP) was determined in human urine by online solid phase extraction–ultra high-performance liquid...
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Hydrolysis of Glycosides
The glycosidic bond might be degraded by chemical and or enzymatic agents. Comparative studies revealed that chemical hydrolysis is nonspecific and...
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Novel and Tailor-Made Enzyme Cocktails for Saccharification of Cellulosic Biomass
Lignocellulosic biomass (LCB), such as agricultural residues and plant-based waste, holds tremendous potential as a renewable and sustainable source...
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Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibility
BackgroundLignin and xylan are important determinants of cell wall structure and lignocellulosic biomass digestibility. Genetic manipulations that...
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Simultaneous determination of chloramphenicol, stilbenes, and resorcylic acid lactones in pork using UPLC–MS/MS with a C18 cartridge and immunoaffinity microextraction in a packed syringe
The aim of this study was to establish an analytical method for the simultaneous detection of chloramphenicol, stilbenes, and resorcylic acid...
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Targeting the human gut microbiome with small-molecule inhibitors
The human gut microbiome is a complex microbial community that is strongly linked to both host health and disease. However, the detailed molecular...
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Determination of the urinary concentrations of six bisphenols in public servants by online solid-phase extraction-liquid chromatography tandem mass spectrometry
Bisphenols are widely used as monomers and additives in plastic production. Thus, bisphenol A (BPA) and its most prominent substitutes have been...
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Cellulose Degradation Enzymes in Filamentous Fungi, A Bioprocessing Approach Towards Biorefinery
The economic exploration of renewable energy resources has hot fundamentals among the countries besides dwindling energy resources and increasing...
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Synthesis, β-Glucuronidase Inhibitory Activity, and Molecular Docking Study of Oxazole Derivatives
AbstractA series of 4-aryl-2-[2-(arylmethylidene)hydrazinyl]-1,3-oxazoles were synthesized from the corresponding aromatic aldehyde semicarbazones...
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Detection of 41 Drugs and Metabolites in Urine with Internal Hydrolysis Control by Liquid Chromatography-Tandem Mass Spectrometry
Regular monitoring of pain management and substance use disorder patients through urine drug screening is important for assessing patient compliance...
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Polysaccharide-Based Materials for the Development of Point-of-Care Devices
Polysaccharide-based materials are increasingly used for the development of point-of-care (POC) devices or some of their components. This chapter...
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Insights into the capability of the lignocellulolytic enzymes of Penicillium parvum 4-14 to saccharify corn bran after alkaline hydrogen peroxide pretreatment
BackgroundCorn bran is a major agro-industrial byproduct from corn starch processing. It contains abundant arabinoxylan that can be converted into...
