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A 4-hydroxybenzoate 3-hydroxylase mutant enables 4-amino-3-hydroxybenzoic acid production from glucose in Corynebacterium glutamicum
BackgroundMicrobial production of aromatic chemicals is an attractive method for obtaining high-performance materials from biomass resources. A...
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Laser Mutagenesis of Phellinus igniarius Protoplasts for the Selective Breeding of Strains with High Laccase Activity
Phellinus igniarius is a medicinal fungus that utilizes lignin as a nutrient substrate. This fungus has a weak lignin degradation ability and, as a...
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The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
BackgroundMicrobial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens...
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Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
BackgroundClassical directed evolution is a powerful approach for engineering biomolecules with improved or novel functions. However, it...
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Cytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagataella phaffii
BackgroundHuman lysozyme (hLYZ) is a natural antibacterial protein with broad applications in food and pharmaceutical industries. Recombinant...
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Site-Directed Mutagenesis in Barley Using RNA-Guided Cas Endonucleases During Microspore-Derived Generation of Doubled Haploids
In plant research and breeding, haploid technology is employed upon crossing, induced mutagenesisMutagenesis or genetic engineering to generate...
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Covalent inhibition of pro-apoptotic BAX
BCL-2-associated X protein (BAX) is a promising therapeutic target for activating or restraining apoptosis in diseases of pathologic cell survival or...
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Substrate scope expansion of 4-phenol oxidases by rational enzyme selection and sequence-function relations
Enzymes are natures’ catalysts and will have a lasting impact on (organic) synthesis as they possess unchallenged regio- and stereo selectivity. On...
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Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis
D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited...
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Enhanced ε‑poly‑l‑lysine production in Streptomyces species by combining interspecific hybridization with multiple antibiotic resistance
To improve the ε-PL production in wild-type strains of Streptomyces. albulus , Streptomyces. noursei , Streptomyces. rochei and Streptomyces. yunnanensis ...
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Control of Fluxes Towards Antibiotics and the Role of Primary Metabolism in Production of Antibiotics
Yield improvements in antibiotic-producing strains have classically been obtained through random mutagenesis and screening. An attractive alternative...
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Artificial switches induce the bespoke production of functional compounds in marine microalgae Chlorella by neutralizing CO2
To improve the CO 2 tolerance of a marine microalga Chlorella sp. of which the production capacity has been demonstrated industrially, a mutant...
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Genetic Code Expansion in Mammalian Cells Through Quadruplet Codon Decoding
Genetic code expansion enables the site-specific incorporation of noncanonical amino acids (ncAAs) into proteins both in vitro and in vivo. In...
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Engineering of cyclodextrin glycosyltransferase improves the conversion efficiency of rebaudioside A to glucosylated steviol glycosides and increases the content of short-chain glycosylated steviol glycoside
BackgroundCompared with steviol glycosides, the taste of glucosylated steviol glycosides is better and more similar to that of sucrose. At present,...
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Analysis of the effect of pulsed light on the protein of Lactobacillus plantarum based on liquid mass spectrometry
Studying the mutagenesis mechanism is crucial for pulsed light use in the food processing industry. After being exposed to pulsed light, the original...
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Development of a yeast cell based method for efficient screening of high yield tacrolimus production strain
Tacrolimus (FK506) is a widely used and clinically important immunosuppressant drug that can be produced by fermentation of Streptomyces tsukubaensis ....
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Antibody–peptide conjugates deliver covalent inhibitors blocking oncogenic cathepsins
Cysteine cathepsins are a family of proteases that are relevant therapeutic targets for the treatment of different cancers and other diseases....
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Improving aptamer performance: key factors and strategies
Aptamers are functional single-stranded oligonucleotide fragments isolated from randomized libraries by Systematic Evolution of Ligands by...
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Scanning the active center of formolase to identify key residues for enhanced C1 to C3 bioconversion
BackgroundFormolase (FLS) is a computationally designed enzyme that catalyzes the carboligation of two or three C1 formaldehyde molecules into C2...
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Immobilization of Bio-imprinted Phospholipase D and Its Catalytic Behavior for Transphosphatidylation in the Biphasic System
Phospholipase D (PLD) with the higher transphosphatidylation activity was screened from Streptomyces sp. LD0501 basing on the protoplast mutagenesis...
