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Improving the specificity of organophosphorus hydrolase to acephate by mutagenesis at its binding site: a computational study
Organophosphorus hydrolase (OPH) is one of the most important enzymes in order to bioremediation of organophosphorus (OP) pesticides. OPH is capable...
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Protein Engineering of Bst Polymerase for Isothermal Amplification Purposes
AbstractThis paper reviews the protein engineering of Bst polymerase using various methods. To modify the enzyme, approaches such as the production...
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Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation
BackgroundL-lysine is widely used for feed and special diet products. The transformation of fermentation strains plays a decisive role in the...
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Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method
Regulation of amino acid’s biosynthetic pathway is of significant importance to maintain homeostasis and cell functions. Amino acids regulate their...
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Site-directed mutagenesis in the P-domain of calreticulin transacylase identifies Lys-207 as the active site residue
In silico-docking studies from previous work have suggested that Lys-206 and lys-207 of calreticulin (CR) play a pivotal key role in its...
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Development of Orthogonal Aminoacyl tRNA Synthetase Mutant with Enhanced Incorporation Ability with Para-azido-L-phenylalanine
Orthogonal aminoacyl tRNA synthetase (aaRS)/tRNA pairs are an efficient tool for the site-specific introduction of para-azido-L-phenylalanine (pAzF),...
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Strategies for Strain Improvement of Economically Important Microorganisms
Microorganisms are sustainable assets for meeting the rising demand of growing industries. However, the wide uses of microbial-derived products are...
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GMOs: A Regulatory Concept
For many people, GMOs have become synonymous with monstrosity, whereas, as we have seen in the previous chapters, research after the creation of...
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Understanding the Structural Requirements of Peptide–Protein Interaction and Applications for Peptidomimetic Development
Protein–protein interaction is at the heart of most biological processes, and small peptides that bind to protein binding sites are resourceful tools...
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CRISPR-Cas-Mediated Optimization of Plant-Derived Natural Products of Biomedical Significance
Medicinal plants have been used since time immemorial to heal mankind on all three tiers, namely, spiritual health, mental health, and physical...
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Key role of K+ and Ca2+ in high-yield ethanol production by S. Cerevisiae from concentrated sugarcane molasses
BackgroundSaccharomyces cerevisiae is an important microorganism in ethanol synthesis, and with sugarcane molasses as the feedstock, ethanol is...
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Production of Enhanced Carotenoid-Producing Strains of the Yeast Rhodotorula gracilis Using the Antibiotic Zeocin
We employed genomic mutagenesis to the yeast Rhodotorula gracilis using Zeocin as a mutagen to develop enhanced carotenoid-producing mutant strains....
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Directed Evolution Methods and Protocols
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle....
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TILLING and Eco-TILLING: Concept, Progress, and Their Role in Crop Improvement
TILLING (Targeting Induced Local Lesions in Genomes) is a rapid, high-throughput, and economical non-transgenic tool of reverse genetics aimed to...
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Strain improvement, artificial intelligence optimization, and sensitivity analysis of asparaginase-mediated acrylamide reduction in sweet potato chips
In recent times, L-asparaginase has emerged as a potential anti-carcinogen through hydrolysis of L-asparagine in the blood for anti-leukemic...
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Plant Breeding from Classical Genetics to Molecular Approaches for Food and Nutrition Security
Concerns over global food security have a significant influence on the UN’s Sustainable Development Goals, which have been primarily centered on...
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Advances and prospects in microbial production of biotin
Biotin, serving as a coenzyme in carboxylation reactions, is a vital nutrient crucial for the natural growth, development, and overall well-being of...
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Investigating the Performance of Machine Learning Methods in Predicting Functional Properties of the Hydrogenase Variants
Improving a functional property of an enzyme via mutagenesis is still a challenging problem due to vast search space and difficulty of predicting the...
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An Efficient Approach for Two Distal Point Site-Directed Mutagenesis from Randomly Ligated PCR Products
Site-directed mutagenesis is one of the most important tools in molecular biology. The majority of the mutagenesis methods have been developed to...
