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Linking Anorexia Nervosa with the Gut Microbiota
In the present chapter, we provide a comprehensive review of the potential role of gut microbiota in the pathogenesis of anorexia nervosa (AN) and...
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Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications
Reconstitution of a complicated system with a minimal set of components is essential for understanding the mechanisms of how the input is reflected...
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Ferulic acid production by metabolically engineered Escherichia coli
Ferulic acid ( p -hydroxy-3-methoxycinnamic acid, FA) is a natural active substance present in plant cell walls, with antioxidant, anticancer,...
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Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast
Advances in synthetic biology enable microbial hosts to synthesize valuable natural products in an efficient, cost-competitive and safe manner....
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A gene-encoded FRET fluorescent sensor designed for detecting asymmetric dimethylation levels in vitro and in living cells
Arginine methylation is involved in many important biological processes. PRMT1 is a major arginine methyltransferase in mammalian cells and is highly...
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Labeling and Detection of Modified Nucleic Acids
The nucleic acids were modified by various chemical groups to regulate gene expression without alteration of primary sequence. Until now, there are...
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Water-Soluble Vitamins
Water-soluble vitamins are a group of essential dietary components required in small quantities that are defined by their polar nature and are hence...
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Cryptic enzymatic assembly of peptides armed with β-lactone warheads
Nature has evolved biosynthetic pathways to molecules possessing reactive warheads that inspired the development of many therapeutic agents,...
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Generation and Characterization of In Vitro Transcribed mRNA
Here we describe the in vitro preparation of mRNA from DNA templates, including setting up the transcription reaction, mRNA cap**, and mRNA...
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Dietary Lipids and Health
Historically as early as 7000 BC, the use of lipids as a cosmetic was well established and later its use as an industrial oil was also done. However,...
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Host Response of Arabidopsis thaliana Interaction with Fungal Endophytes Involves microRNAs
Plant and fungus interaction is a complex process involving many molecular factors determining the nature of relationship. The enigmatic methodology...
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HpaII-assisted and linear amplification-enhanced isothermal exponential amplification fluorescent strategy for rapid and sensitive detection of DNA methyltransferase activity
The detection of methyltransferase (MTase) activity is of great significance in methylation-related disease diagnosis and drug screening. Herein, a...
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Identification of PRMT5 inhibitors with novel scaffold structures through virtual screening and biological evaluations
Protein arginine methyltransferase 5 (PRMT5), an important member in PRMT family, has been validated as a promising anticancer target. In this study,...
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Mechanistic basis of the increased methylation activity of the SETD2 protein lysine methyltransferase towards a designed super-substrate peptide
Protein lysine methyltransferases have important regulatory functions in cells, but mechanisms determining their activity and specificity are...
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Emerging methylation-based approaches in microbiome engineering
Bacterial epigenetics, particularly through DNA methylation, exerts significant influence over various biological processes such as DNA replication,...
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Synthetic auxotrophs accelerate cell factory development through growth-coupled models
The engineering of microbial cell factories for the production of high-value chemicals from renewable resources presents several challenges,...
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Indigenous Plants Against Cytomegalovirus, Cytomegalovirus B1, and Epstein-Barr Virus Infection: Phytochemical, Clinical, and Preclinical Studies and the Prevalence and Impact on Patients with Hematological Diseases
Cytomegalovirus is a common virus that causes congenital illnesses. The lytic infection of the Epstein-Barr virus (EBV) has a substantial role in...
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Homogeneous luminescent quantitation of cellular guanosine and adenosine triphosphates (GTP and ATP) using QT-LucGTP&ATP assay
Guanosine triphosphate (GTP) and adenosine triphosphate (ATP) are essential nucleic acid building blocks and serve as energy molecules for a wide...
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Broadening the Scope of Biocatalysis Engineering by Tailoring Enzyme Microenvironment: A Review
The rational design of catalysts that fine-tune/mimics the enzyme microenvironment remains the subject of supreme interest. Several strategies moving...
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Microbial Production of Vitamin B12 Using Food Matrices
Vitamin B12 (Vit B12) is a commonly consumed ingredient in food and feed, health care products and is produced by fermentation due to its complexity...