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High oil accumulation in tuber of yellow nutsedge compared to purple nutsedge is associated with more abundant expression of genes involved in fatty acid synthesis and triacylglycerol storage
BackgroundYellow nutsedge is a unique plant species that can accumulate up to 35% oil of tuber dry weight, perhaps the highest level observed in the...
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Quantification and mitigation of byproduct formation by low-glycerol-producing Saccharomyces cerevisiae strains containing Calvin-cycle enzymes
BackgroundAnaerobic Saccharomyces cerevisiae cultures require glycerol formation to re-oxidize NADH formed in biosynthetic processes. Introduction...
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Microbial Production of Diamines
Diamines have the general structure of a carbon backbone with two amino groups. Their applications in industry are very diverse since they can be... -
Improvement of Lipid and Terpenoid Yield in Thraustochytrids Using Chemical Regulators: A Review
For the increasing demand for lipids and terpenoids in humans, biological fermentation has become an attractive choice due to the safety and...
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Current status of carbon monoxide dehydrogenases (CODH) and their potential for electrochemical applications
Anthropogenic carbon dioxide (CO 2 ) levels are rising to alarming concentrations in earth’s atmosphere, causing adverse effects and global climate...
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Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO2 capture
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key CO 2 -fixing enzyme in photosynthesis, is notorious for its low carboxylation. We...
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Dual stress factors adaptive evolution for high EPA production in Schizochytrium sp. and metabolomics mechanism analysis
Eicosapentaenoic acid (EPA) is a vital ɷ-3 polyunsaturated fatty acid (PUFA) for human body with various physiological functions. In this study, we...
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RuBisCO activity assays: a simplified biochemical redox approach for in vitro quantification and an RNA sensor approach for in vivo monitoring
BackgroundRibulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the most abundant soluble protein in nature. Extensive studies have been...
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Interplay between photosynthetic electron flux and organic carbon sinks in sucrose-excreting Synechocystis sp. PCC 6803 revealed by omics approaches
BackgroundAdvancing the engineering of photosynthesis-based prokaryotic cell factories is important for sustainable chemical production and requires...
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Metabolic Engineering for Redirecting Carbon to Enhance the Fatty Acid Content of Synechocystis sp. PCC6803
Biofuels produced by photosynthetic microorganisms are considered a renewable, sustainable, and eco-friendly alternative to fossil fuels that cause a...
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Metabolomic, proteomic and lactylated proteomic analyses indicate lactate plays important roles in maintaining energy and C:N homeostasis in Phaeodactylum tricornutum
BackgroundPhaeodactylum tricornutum accumulates lipids while the growth also increases under high CO 2 , shedding light on its potential application...
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Introduction to Cyanobacteria
Cyanobacteria are highly interesting microbes with the capacity for oxygenic photosynthesis. They fulfill an important purpose in nature but are also... -
Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds
Methane is considered the carbon source with the highest potential in industrial biotechnology because of its abundance and sustainability. The... -
Enzymatic CO2 Conversion
The ever-growing energy and chemical demand have resulted in the absurd usage of fossil fuels, which has led to a significant increase in the CO2... -
Engineering yeast mitochondrial metabolism for 3-hydroxypropionate production
BackgroundWith unique physiochemical environments in subcellular organelles, there has been growing interest in harnessing yeast organelles for...
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Enzymes
Enzymes are proteins that act as biological catalysts, i.e. these alter the rates of biochemical reactions without undergoing any permanent change in... -
Synthesis of Polyols and Organic Acids by Wild-Type and Metabolically Engineered Yarrowia lipolytica Strains
In the yeast Yarrowia lipolytica, sugar polyols and organic acids are derived from central metabolism, namely the citrate cycle and the pentose... -
Physiochemical and molecular responses of the diatom Phaeodactylum tricornutum to illumination transitions
BackgroundLight is a key regulatory factor for photosynthesis and metabolism of microalgae. The diatom Phaeodactylum tricornutum is capable of...
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Amino Acids
Since discovery of Corynebacterium glutamicum in 1956, amino acid production by microbial fermentation is one of most successful biotechnology models... -
Metabolic Flux Analysis Based on 13C-Labeling Experiments and Integration of the Information with Gene and Protein Expression Patterns
The recent progress on metabolic systems engineering was reviewed, in particular focusing on the metabolic flux analysis (MFA) based on the...