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Increased stable integration efficiency in CHO cells through enhanced nuclear localization of Bxb1 serine integrase
BackgroundMammalian display is an appealing technology for therapeutic antibody development. Despite the advantages of mammalian display, such as...
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A Novel Nanobody Scaffold Optimized for Bacterial Expression and Suitable for the Construction of Ribosome Display Libraries
Single-domain antigen-binding fragments of camelid antibodies, known as VHHs or nanobodies, are widely used affinity reagents. However, their...
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Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired Properties
Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation...
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The next generation of biopanning: next gen sequencing improves analysis of bacterial display libraries
BackgroundBacterial surface display libraries are a popular tool for novel ligand discovery due to their ease of manipulation and rapid growth rates....
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CRISPR-Cas Technology: A Genome-Editing Powerhouse for Molecular Plant Breeding
Global human population is growing rapidly. The latest world population projections indicate human...
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Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody
BackgroundCyclin-dependent kinase 4 (CDK4) when hyperactivated drives development and maintenance of most tumour types, thus prompting its use as an...
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Cardiac Targeting Peptide : From Identification to Validation to Mechanism of Transduction
Cell-penetrating peptides (CPPs), also known as protein transduction domains, were first identified 25 years ago. They are small, ~6–30 amino acid...
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Ligand-Directed GPCR Antibody Discovery
Develo** affinity reagents recognizing and modulating G-protein coupled receptors (GPCR) function by traditional animal immunization or in vitro...
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A History of Selection-Based High-Throughput Screening Technologies for Hit Identification
DNA-encoded libraries (DELs) are now used extensively in both academic and commercial laboratories for the discovery of protein ligands. DEL...
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Efficient Surface Display of L-glutamate Oxidase and L-amino Acid Oxidase on Pichia pastoris Using Multi-copy Expression Strains
L-glutamate oxidase (GLOD) and L-amino acid oxidase (AAO) were reported to be capable of convert L-glutamic acid to α-aketoglutaric acid (α-KG)....
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Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains
Antibody therapeutics have become a cornerstone of the pharmaceutical market due to their precise molecular targeting, favorable pharmacokinetic...
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Forty years of advances in optical biosensors—are “autonomous” biosensors in our future?
Optical biosensors have employed at least three distinct system architectures over the last 40 years, moving from “sample in-answer out” systems to...
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Development of hybrid biomicroparticles: cellulose exposing functionalized fusion proteins
BackgroundOne of the leading current trends in technology is the miniaturization of devices to the microscale and nanoscale. The highly advanced...
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Chimeric Protein Switch Biosensors
Rapid detection of protein and small-molecule analytes is a valuable technique across multiple disciplines, but most in vitro testing of biological...
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Perspectives and trends in advanced optical and electrochemical biosensors based on engineered peptides
With the advancement of life medicine, in vitro diagnostics (IVD) technology has become an auxiliary tool for early diagnosis of diseases. However,...
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A nanobody‐horseradish peroxidase fusion protein‐based competitive ELISA for rapid detection of antibodies against porcine circovirus type 2
BackgroundThe widespread popularity of porcine circovirus type 2(PCV2) has seriously affected the healthy development of the pig industry and caused...
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The Discovery of Peptide Macrocycle Rescuers of Pathogenic Protein Misfolding and Aggregation by Integrating SICLOPPS Technology and Ultrahigh-Throughput Screening in Bacteria
The phenomenon of protein misfolding and aggregation has been widely associated with numerous human diseases, such as Alzheimer’s disease, systemic...
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Recent advances of CREKA peptide-based nanoplatforms in biomedical applications
Nanomedicine technology is a rapidly develo** field of research and application that uses nanoparticles as a platform to facilitate the diagnosis...
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Biomedical Application of Industrial Microbiology
Recombinant DNA (rDNA) technology advancements over the past few decades have completely changed the field of microbial biotechnology by altering...
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Methods for CPP Selection, Prediction and in Silico Analysis
This Chapter summarizes the methods for (initial) CPP selection, identification or design of CPPs by predictionPrediction. These studies deal mainly...