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Purification and Characterization of Trehalase From Acyrthosiphon pisum, a Target for Pest Control
Insect trehalases are glycoside hydrolases essential for trehalose metabolism and stress resistance. We here report the extraction and purification...
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Insights into the cellulose degradation mechanism of the thermophilic fungus Chaetomium thermophilum based on integrated functional omics
BackgroundLignocellulose is the most abundant and renewable biomass resource on the planet. Lignocellulose can be converted into biofuels and...
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Stability Enhancement of Target Enzymes via Tyrosinase-Mediated Site-Specific Polysaccharide Coating
Enzymes are widely used in industrial and pharmaceutical applications, but their activities often decrease rapidly under harsh environmental...
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Exploring the sequence-function space of microbial fucosidases
Microbial α- l- fucosidases catalyse the hydrolysis of terminal α- l -fucosidic linkages and can perform transglycosylation reactions. Based on sequence...
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In silico evaluation of a targeted metaproteomics strategy for broad screening of cellulolytic enzyme capacities in anaerobic microbiome bioreactors
BackgroundMicrobial-driven solubilization of lignocellulosic material is a natural mechanism that is exploited in anaerobic digesters (ADs) to...
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Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24
BackgroundAn important conceptual advance in health and the environment has been recognized that enzymes play a key role in the green processing...
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Enzymatic self-assembly/disassembly turns “ON”/“OFF” the mimetic hydrolytic activity of histidine nanofibers
Mimetic enzymes are devised as alternates or supplements of natural enzymes in broad fields but regulating their activities in a switchable manner...
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Characterization of a highly xylose tolerant β-xylosidase isolated from high temperature horse manure compost
BackgroundThere is a continued need for improved enzymes for industry. β-xylosidases are enzymes employed in a variety of industries and although...
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Degradation of Staphylococcus aureus Biofilm Using Hydrolytic Enzymes Produced by Amazonian Endophytic Fungi
Microbial biofilms can cause serious health problems, since, due to their persistent character, they often function as spreaders of contaminants....
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Computational studies of polyurethanases from Pseudomonas
Polyurethanes (PU) are multifunctional polymers, used in automotive industry, in coatings, rigid and flexible foams, and also in biomimetic...
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Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display
Bacterial cis -epoxysuccinic acid hydrolases (CESHs) are intracellular enzymes used in the industrial production of enantiomeric tartaric acids. The...
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The reaction mechanism of the Ideonella sakaiensis PETase enzyme
Polyethylene terephthalate (PET), the most abundantly produced polyester plastic, can be depolymerized by the Ideonella sakaiensis PETase enzyme....
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Marine Bacterial Esterases: Emerging Biocatalysts for Industrial Applications
The marine ecosystem has been known to be a significant source of novel enzymes. Esterase enzymes (EC 3.1.1.1) represent a diverse group of...
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Trends and Challenges in Enzyme Technology
Several major developments took place in the field of biocatalysis over the past few years. These include the invention of directed evolution... -
Enzymatic post-consumer poly(ethylene terephthalate) (PET) depolymerization using commercial enzymes
Poly(ethylene terephthalate) (PET) is a synthetic polymer widely used globally. The high PET resistance to biotic degradation and its improper...
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Sequence Engineering of an Aspergillus niger Tannase to Produce in Pichia pastoris a Single-Chain Enzyme with High Specific Activity
Tannin acyl hydrolases or tannases (E.C.3.1.1.20) are enzymes that hydrolyze the ester bond of tannins to produce gallic acid and glucose. We...
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The hemicellulose-degrading enzyme system of the thermophilic bacterium Clostridium stercorarium: comparative characterisation and addition of new hemicellulolytic glycoside hydrolases
BackgroundThe bioconversion of lignocellulosic biomass in various industrial processes, such as the production of biofuels, requires the degradation...
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A highly contiguous reference genome assembly for Colletotrichum falcatum pathotype Cf08 causing red rot disease in sugarcane
Among the biotic factors, which affect the productivity and quality of sugarcane, red rot disease caused by the fungal pathogen, Colletotrichum...