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Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trap** for the Detection of Transient Protein Interactions
Antibody-based affinity purification is a recognized method for use in studying protein–protein interactions. There are four different classes of...
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Establishment of liquid phase double antibody radioimmunoassay system for in-vitro determination of erythropoietin hormone in human serum
The aim of study was the production of polyclonal antibody of erythropoietin hormone (Anti-EPO) to determine EPO in human serum by liquid-phase...
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Protein purification strategies must consider downstream applications and individual biological characteristics
BackgroundProteins are used as reagents in a broad range of scientific fields. The reliability and reproducibility of experimental data will largely...
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Excellent removal of knob-into-hole bispecific antibody byproducts and impurities in a single-capture chromatography
Bispecific antibodies (bsAbs) are therapeutically promising due to their ability to bind to two different antigens. However, the bsAb byproducts and...
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Enhanced synthesis of antibody-functionalized gold nanoparticles for multiplexed exosome detection via mass signal amplification in LDI-TOF MS
We present a novel method for sensitive exosomal protein detection using organic matrix-free laser desorption/ionization time-of-flight mass...
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An improved workflow for the development of MS-compatible liquid chromatography assay purity and purification methods by using automated LC Screening instrumentation and in silico modeling
The development of liquid chromatography UV and mass spectrometry (LC-UV–MS) assays in pharmaceutical analysis is pivotal to improve quality control...
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Isorhamnetin and anti-PD-L1 antibody dual-functional mesoporous silica nanoparticles improve tumor immune microenvironment and inhibit YY1-mediated tumor progression
BackgroundThe immune checkpoint inhibitor (ICI) anti-PD-L1 monoclonal antibody can inhibit the progress of hepatocellular carcinoma (HCC)....
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Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics
BackgroundLeukocyte phytohemagglutinin (PHA-L), derived from the L4 tetramer of PHA, has been frequently employed as a mitogen to induce T lymphocyte...
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Multiplexed Bead-Based Peptide Immunoassays for the Detection of Antibody Reactivities
Antigenic peptides are commonly used in serological test settings such as enzyme-linked immunosorbent assays (ELISA) to determine reactive antibodies...
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Affinity-based and in a single step purification of recombinant horseradish peroxidase A2A isoenzyme produced by Pichia pastoris
Horseradish peroxidase (HRP) is an oxidoreductase enzyme and oxidizes various inorganic and organic compounds. It has wide application areas such as...
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Production of α-Synuclein Fibrillar-Specific scFv from Inclusion Bodies
Recombinant antibody fragments such as Fab, scFvs, and diabodies against α-syn have become a viable alternative to the conventional full-length...
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Development of an antibody-ligand fusion protein scFvCD16A-sc4-1BBL in Komagataella phaffii with stimulatory activity for Natural Killer cells
BackgroundNatural killer (NK) cell-based immunotherapies have demonstrated substantial potential for the treatment of hematologic malignancies....
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Short Read-Length Next Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs
Phage display is commonly used to select target-binding antibody fragments from large libraries containing billions of unique antibody clones. In...
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Characterization of a broadly cross reactive tetravalent human monoclonal antibody, recognizing conformational epitopes in receptor binding domain of SARS-CoV-2
We used human semi-synthetic phage antibody gene libraries to select anti-SARS-CoV-2 RBD scFv antibody fragment and subsequent characterization of...
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Rapid and Selective Detection of Foodborne Pathogens Using a Disposable Bio-sensing System Designed by Stepwise Antibody Immobilization on AuNPs@Cu-MOF Nanocomposite
There is an urgent need to provide a rapid and selective method to analyze food and water samples contaminated with Staphylococcus aureus since it is...
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Electrochemical immunosensor for the detection of anti-thyroid peroxidase antibody by gold nanoparticles and ionic liquid-modified carbon paste electrode
In this work, a sandwich-type electrochemical immunosensor was designed based on the modification of the carbon paste electrode consisting of ionic...
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Accessing Antibody Reactivities in Serum or Plasma to (Auto-)antigens Using Multiplexed Bead-Based Protein Immunoassays
Antibody (AB) testing or serotesting for reactive ABs against antigenic proteins is broadly used. Parallel examination of many antigens is of high...
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Purification of HLA Immunopeptidomes from Human Thymus
Mass spectrometry has become an essential technique for the analysis of peptide repertoires presented by MHC molecules to T lymphocytes. Years ago,...
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Aptamer decorated PDA@magnetic silica microparticles for bacteria purification
One significant constraint in the advancement of biosensors is the signal-to-noise ratio, which is adversely affected by the presence of interfering...
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Facile and Rapid Detection of Microalbuminuria by Antibody-Functionalized Gold Nanorods
Any excess or lack of human serum albumin (HSA) in urine should be considered a warning for renal or cardiovascular diseases. This report proposes a...
