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m6A mRNA methylation initiated by METTL3 inhibits KDR translation to increase the efficacy of 131I therapy in papillary thyroid carcinoma
Papillary thyroid carcinoma (PTC) is a common malignant tumor. 131 I treatment can effectively kill residual PTC microtissues and inhibit metastases....
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Understanding Pseudophosphatase Function Through Biochemical Interactions
Pseudophosphatases have been solidified as important signaling molecules that regulate signal transduction cascades. However, their mechanisms of...
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Delivery of progeny virus from the infectious clone of cucumber green mottle mosaic virus and quantification of the viral load in different host plants
Cucumber green mottle mosaic virus (CGMMV, genus Tobamovirus ) is a widely occurring tobamovirus in cucurbits. The genome of CGMMV has been used...
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QPCR-Guided Screening of Levansucrase: Levan Characterization and Genomic Insights
Eighty-seven bacteria isolates were screened for the presence of the levansucrase encoding gene and those possessing the gene were used in gene...
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Analyses of the Posttranscriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression
Cells generally control the concentration of mRNA via transcriptional and posttranscriptional regulation, so the separate contributions of synthesis...
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Digital PCR: A Tool to Authenticate Herbal Products and Spices
Authentication of herbal products and spices is experiencing a resurgence using DNA-based molecular tools, mainly species-specific assays and DNA...
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A Method Based on a Modified Fluorescence In Situ Hybridization (FISH) Approach for the Sensing of Staphylococcus aureus from Nasal Samples
Staphylococcus aureus is a major source of bacteremia and develops several complications, causing high morbidity and mortality. Rapid identification...
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Real-time Loop-Mediated Isothermal Amplification (LAMP) Using Self-quenching Fluorogenic Probes: the Application in Skipjack Tuna (Katsuwonus pelamis) Authentication
Target-specific detection of LAMP amplicons can be achieved through the use of target-specific probes or modified primers as biorecognition elements,...
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Bone marrow-targetable Green Tea Catechin-Based Micellar Nanocomplex for synergistic therapy of Acute myeloid leukemia
BackgroundCurrently available anti-leukemia drugs have shown limited success in the treatment of acute myeloid leukemia (AML) due to their poor...
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Protein Quantitation and Analysis of Purity
The accurate quantitation of proteins and an analysis of their purity is essential in numerous areas of scientific research and is a critical factor...
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Loop-Mediated Isothermal Amplification for On-Site Visual Identification of Leech Species
Leeches are a well-known animal-derived health supplement commonly used as an anticoagulation and antithrombosis agent; however, adulteration and...
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Modified loop-mediated isothermal amplification method combined with a TaqMan probe for the detection of Mycoplasma gallisepticum
We established and validated an improved loop-mediated isothermal amplification (LAMP) method combined with a TaqMan probe (LAMp-Taqman method) for...
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MicroRNA-483-3p Inhibitor Ameliorates Sepsis-Induced Intestinal Injury by Attenuating Cell Apoptosis and Cytotoxicity Via Regulating HIPK2
Sepsis is a life-threatening syndrome that can result in multi-organ dysfunction. MicroRNA (miR)-483-3p was previously demonstrated to be upregulated...
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Rapid detection of four major HFMD-associated enteroviruses by multiplex HiFi-LAMP assays
Hand, foot, and mouth disease (HFMD) caused by various enteroviruses is a major public health concern globally. Human enterovirus 71(EVA71),...
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Development of a loop-mediated isothermal amplification (LAMP) assay for the identification of the invasive wood borer Aromia bungii (Coleoptera: Cerambycidae) from frass
The red-necked longhorn beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) is native to east Asia, where it is a major pest of...
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Hypothalamic expression of GnRH-I and GnIH in the Eurasian tree sparrow over a single long day
Seasonal reproductive cycles of most birds are regulated by photoperiod via neuroendocrine control. The present study aims to investigate the role of...
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CTSB Nuclear Translocation Facilitates DNA Damage and Lysosomal Stress to Promote Retinoblastoma Cell Death
Retinoblastoma (RB) is a pernicious tumor originating from photoreceptor precursor cells that often endangers the lives of children. The purpose of...
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Mayaro virus detection by integrating sample preparation with isothermal amplification in portable devices
Mayaro virus (MAYV) is an emerging mosquito-borne alphavirus that causes clinical symptoms similar to those caused by Chikungunya virus (CHIKV),...
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Regulation of CCN2 and Its Bioactivity by Advanced Glycation End Products
Advanced glycation end products (AGEs) have been implicated in the tissue fibrosis and extracellular matrix (ECM) expansion in organ complications of...
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Leucine and arginine enhance milk fat and milk protein synthesis via the CaSR/Gi/mTORC1 and CaSR/Gq/mTORC1 pathways
Background and aimsAmino acids (AAs) not only constitute milk protein but also stimulate milk synthesis through the activation of mTORC1 signaling,...
