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Detection of salinomycin and lasalocid in chicken liver by icELISA based on functional bispecific single-chain antibody (scDb) and interpretation of molecular recognition mechanism
Salinomycin (SAL) and lasalocid (LAS) are widely used as ionophore antibiotics for coccidiosis control. However, their common use as feed additives...
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Development of single- and multiplex immunoassays for rapid detection and quantitation of amodiaquine in ACT drugs and rat serum
Amodiaquine (AQ) is a commonly used antimalarial drug, and N -desethyl-AQ (N-DEAQ) is an active metabolite of AQ. Given the significance of drug...
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Screening of monoclonal antibodies against specific phosphonylation sites and analysis of serum samples exposed to soman and VX using an indirect competitive enzyme-linked immunosorbent assay
Organophosphorus nerve agents (OPNAs) covalently bind to tyrosine 411 of human serum albumin (HSA) and the formed adducts are stable biomarkers of...
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Production of monoclonal antibody against tylosin and tilmicosin with homogeneous cross-reactivity and its application in lateral flow immunoassay
To avoid false negative results due to the low cross-reactivity rate (CR) in rapid immunoassay, a group-specific antibody with homogeneous CR toward...
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Development of a Novel Monoclonal Antibody–Based Indirect Competitive ELISA with Immunoaffinity Cleanup for the Detection of Triclosan in Chickens
Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive...
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Recombinant antibodies and their use for food immunoanalysis
Antibodies are widely employed as biorecognition elements for the detection of a plethora of compounds including food and environmental contaminants,...
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Production of a specific monoclonal antibody and a sensitive immunoassay for the detection of diphacinone in biological samples
Diphacinone (DPN) is an extensively used anticoagulant rodenticide that is also considered a hazardous chemical, which poses a threat to nontarget...
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Development of a magnetic separation immunoassay with high sensitivity and time-saving for detecting aflatoxin B1 in agricultural crops using nanobody
Detection methods with high sensitivity and short assay time are urgently required for quantitative analysis of small-molecule hazardous substances...
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An aggregation-induced emission immunoassay for broad detection of polychlorinated biphenyls in chicken and crab
Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with multiple variants, which may be harmful to human health by absorption...
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A novel high-sensitive indirect competitive chemiluminescence enzyme immunoassay based on monoclonal antibody for tenuazonic acid (TeA) detection
Tenuazonic acid (TeA), as a naturally occurring mycotoxin found in sorghum, corn, wheat, oat, tomato, apple, rapeseed and their products, has...
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Development of a Highly Sensitive Biotin-Streptavidin Amplified Enzyme-Linked Immunosorbent Assay for Determination of Progesterone in Milk Samples
Agricultural product contamination by endocrine-disrupting compounds (EDCs) residues is an international public health issue and requires...
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Site-directed mutations of anti-amantadine scFv antibody by molecular dynamics simulation: prediction and validation
A recombinant single-chain variable fragment (scFv) antibody was produced from a hybridoma cell strain secreting the monoclonal antibody for...
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Novel hapten design, antibody recognition mechanism study, and a highly sensitive immunoassay for diethylstilbestrol in shrimp
Over the past few years, there has been a lack of progress in the quality of diethylstilbestrol (DES) antibodies used in immunoassay. In this study,...
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Improvement of stilbene production by mulberry Morus alba root culture via precursor feeding and co-elicitation
Large amounts of Morus alba L. (MA) roots are needed as the source of active stilbenes in the industrial production of traditional medicines and...
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Fluorescent lateral flow immunoassay based on gold nanocluster for detection of pyrrolizidine alkaloids
An ultrasensitive and rapid fluorescent immunoassay based on a broad-spectrum monoclonal antibody (mAb) was developed to detect pyrrolizidine...
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Binding properties of broad-specific monoclonal antibodies against three organophosphorus pesticides by a direct surface plasmon resonance immunosensor
In this study, heterologous indirect competitive enzyme-linked immunosorbent assay (icELISA) was introduced into the screening of hybridomas for the...
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Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzyme-mediated transformation of Pueraria mirifica isoflavones: a sustainable approach for increasing health-bioactive constituents
The presence of specific gut microflora limits the biotransformation of Pueraria mirifica isoflavone (PMI) glycosides into absorbable aglycones, thus...
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Development of an icELISA and Immunochromatographic Assay for Methyl-3-Quinoxaline-2-Carboxylic Acid Residues in Fish
To evaluate residues of olaquindox (OLA) and its marker compound methyl-3-quinoxaline-2-carboxylic acid (MQCA) in aquatic products in the field, five...
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Comparison of Chicken IgY and Mammalian IgG in Three Immunoassays for Detection of Sulfamethazine in Milk
Antibodies are the most important reagents for the development of highly sensitive and specific immunoassays to quantify analytes of interest in food...
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Detection methods for antibiotics in wastewater: a review
Antibiotics are widely used as fungicides because of their antibacterial and bactericidal effects. However, it is necessary to control their dosage....
