79 Result(s)
within
Chemistry
2012 - 2012
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Book
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Book
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Book
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Book
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Protocol
A Method to Evaluate Biomass Accessibility in Wet State Based on Thermoporometry
The substrate accessibility to enzyme has been considered as one of the most important factors for biomass conversion. To avoid the irreversible collapse of pore structure during the drying of sample, the meas...
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Protocol
A Simple Method for Determining Specificity of Carbohydrate-Binding Modules for Purified and Crude Insoluble Polysaccharide Substrates
Experimental identification of carbohydrate-binding modules (CBM) and determination of ligand specificity of each CBM are complementary and compulsory steps for their characterization. Some CBMs are very speci...
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Protocol
Affinity Electrophoresis as a Method for Determining Substrate-Binding Specificity of Carbohydrate-Active Enzymes for Soluble Polysaccharides
Affinity electrophoresis is a simple and rapid tool for the analysis of protein-binding affinities to soluble polysaccharides. This approach is particularly suitable for the characterization of the carbohydrat...
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Protocol
Imaging Cellulose Using Atomic Force Microscopy
Cellulose is an important biopolymer primarily stored as plant cell wall material. Plant-synthesized cellulose forms elementary fibrils that are micrometers in length and 3–5 nm in dimensions. Cellulose is a d...
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Analyzing Single DNA Molecules by Nanopore Translocation
Small holes in membranes or nanocapillaries can be employed to detect single molecules in solution. In fact, the resistive-pulse technique based on nanopores allows for determination of length, charge, and fol...
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Protocol
Immunological Approaches to Plant Cell Wall and Biomass Characterization: Immunolocalization of Glycan Epitopes
Plant cell walls are dynamic structures that show changes in composition and configuration depending on the developmental stage, biotic, and abiotic factors. Therefore, it is necessary to have tools for visual...
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Cellulase Processivity
There are two types of processive cellulases, exocellulases and processive endoglucanases. There are also two classes of exocellulases, ones that attack the reducing ends of cellulose chains and ones that atta...
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Protocol
Bacterial Cadherin Domains as Carbohydrate Binding Modules: Determination of Affinity Constants to Insoluble Complex Polysaccharides
Cadherin (CA) and cadherin-like (CADG) doublet domains from the complex polysaccharide-degrading marine bacterium, Saccharophagus degradans 2–40, demonstrated reversible calcium-dependent binding to different com...
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Micro/Nanopatterning of Proteins Using a Nanoimprint-Based Contact Printing Technique
Micro and nanoscale protein patterning based on microcontact printing technique on large substrates have often resolution problems due to roof collapse of the poly(dimethylsiloxane) (PDMS) stamps used. Here, w...
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Structural Characterization of the Heteroxylans from Poplar and Switchgrass
Heteroxylans are polysaccharides with a backbone composed of 1,4-linked β-d-xylosyl residues. In hardwoods some of these xylosyl residues are substituted at O-2 with 4-O-methyl α-d-glucuronic and occasionally wit...
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Protocol
Measurements of DNA Immobilized in the Alpha-Hemolysin Nanopore
In the past decade, there have been extensive studies aimed at exploring the potential of protein nanopores to sequence single strands of DNA using resistive pulse sensing. The high speed of DNA electrophoreti...
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A Novel Bone Scaffold Design Approach Based on Shape Function and All-Hexahedral Mesh Refinement
Tissue engineering is the application of interdisciplinary knowledge in the building and repairing of tissues. Generally, an engineered tissue is a combination of living cells and a support structure called a ...
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Protocol
Overview of Computer Modeling of Cellulose
Although it has a deceptively simple primary structure, the collective organization of bulk cellulose, particularly as it exists in cellulose fibers in the cell walls of living plants and other organisms, is q...
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Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling
The native complexity of plant cell walls makes research on them challenging. Hence, it is advantageous to have a diversity of tools that can be used to analyze and characterize plant cell walls. In this chapt...
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Protocol
A Neural Network Technique for Remeshing of Bone Microstructure
Today, there is major interest within the biomedical community in develo** accurate noninvasive means for the evaluation of bone microstructure and bone quality. Recent improvements in 3D imaging technology,...
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Protocol
Measuring Single-Wall Carbon Nanotubes with Solid-State Nanopores
Solid-state nanopores have been used widely to study biological polymers. Here, we expand the technique to analyze single-wall carbon nanotubes. By wrap** them in an amphiphilic layer, individual tubes can b...
