Search
Search Results
-
-
Polymerase Chain Reaction: An Indispensable Molecular Biology Tool of 21st Century
Due to its universal applications, Polymerase Chain Reaction (PCR) became an essential technique in various scientific branches such as microbiology,...
-
Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes
BackgroundTheileria equi causes equine piroplasmosis, an economically significant disease that affects horses and other equids worldwide. Based on...
-
Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods
Leishmania infections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world’s poorest regions....
-
Multiplex Quantitative Polymerase Chain Reaction Test to Identify SARS-CoV-2 Variants
Quantitative polymerase chain reaction (qPCR) is a routinely used method for detection and quantitation of gene expression in real time. This is...
-
Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method
PurposeThis study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR)...
-
Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses
The reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is considered to be the gold standard for gene expression...
-
Development and application of a real-time polymerase chain reaction assay to detect lumpy skin disease virus belonging to the Kenyan sheep and goat pox group
Lumpy skin disease (LSD) outbreaks in Southeast and South Asia are attributed to different lineages of LSD virus (LSDV). Variants belonging to the...
-
-
Diagnosis of Human Cutaneous Leishmaniasis: A Comparative Study Using CL Detect™ Dipstick, Direct Smear and Polymerase Chain Reaction Methods
IntroductionIn most of the endemic areas, the detection of CL is based on searching for amastigotes using the direct smear method. Since expert...
-
Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction
Real-time polymerase chain reaction (PCR) for the detection of African swine fever virusAfrican swine fever virus (ASFV) is the tool of choice for...
-
Magnetic-bead-based DNA-capture-assisted real-time polymerase chain reaction and recombinase polymerase amplification for the detection of African swine fever virus
African swine fever (ASF) is a deadly disease in swine caused by African swine fever virus (ASFV). The global spread of ASFV has resulted in...
-
Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses
COVID-19 disease caused by the novel SARS-CoV-2 virus represents a new challenge for healthcare systems. The molecular confirmation of infection is...
-
Evaluation of a probe hybridization quantitative polymerase chain reaction assay for Cryptosporidium serpentis in eastern indigo snakes (Drymarchon couperi)
A probe-hybridization quantitative polymerase chain reaction assay specific for Cryptosporidium serpentis (qPCR) has been developed and shown to be...
-
Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases
Polymerase spiral reaction is a novel and emerging isothermal nucleic acid-based amplification assay for the detection and diagnosis of microbial...
-
Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction
BackgroundThe development of an influenza RNA-dependent RNA polymerase (RdRp) inhibitor is required; therefore, a method for evaluating the activity...
-
A multiplex real-time reverse transcription polymerase chain reaction assay for differentiation of classical and variant II strains of avian infectious bronchitis virus
Identification of avian infectious bronchitis virus (IBV) genotypes is essential for controlling infectious bronchitis (IB) disease, because vaccines...
-
First report on knockdown resistance mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high-resolution melting polymerase chain reaction method
BackgroundThe mosquito Aedes aegypti is an urban vector of dengue and other arboviruses. During epidemics of these viruses, pyrethroid insecticides...
-
Development of a droplet digital polymerase chain reaction tool for the detection of Toxoplasma gondii in meat samples
Toxoplasmosis is a zoonotic disease caused by the protozoan parasite Toxoplasma gondii . Infection in humans has usually been related to the...
