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CRISPR Off-Target Analysis Platforms
The clustered regularly interspaced short palindromic repeats (CRISPR)-Caspase9 (Cas9) system provides a programmable technology that may be used to...
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Human genetic diversity alters off-target outcomes of therapeutic gene editing
CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat disease. However, standard computational and biochemical...
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AttSiOff: a self-attention-based approach on siRNA design with inhibition and off-target effect prediction
Small interfering RNA (siRNA) is often used for function study and expression regulation of specific genes, as well as the development of small...
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Affinity fine-tuning anti-CAIX CAR-T cells mitigate on-target off-tumor side effects
One of the major hurdles that has hindered the success of chimeric antigen receptor (CAR) T cell therapies against solid tumors is on-target...
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Off-target activity of NBOMes and NBOMe analogs at the µ opioid receptor
New psychoactive substances (NPS) are introduced on the illicit drug market at a rapid pace. Their molecular targets are often inadequately...
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Reduction of Off-Target Effects of Gapmer Antisense Oligonucleotides by Oligonucleotide Extension
AimAntisense oligonucleotide (ASO) has the potential to induce off-target effects by inadvertent binding of ASOs to unintended RNAs that have a...
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Fcγ Receptor-Dependent Internalization and Off-Target Cytotoxicity of Antibody-Drug Conjugate Aggregates
PurposeAntibody-drug conjugates (ADCs), which are monoclonal antibodies (mAbs) conjugated with highly toxic payloads, achieve high tumor killing...
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Pharmacological inhibitors of the cystic fibrosis transmembrane conductance regulator exert off-target effects on epithelial cation channels
The cystic fibrosis transmembrane conductance regulator (CFTR) anion channel and the epithelial Na + channel (ENaC) play essential roles in...
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Characterizing off-target corticospinal responses to double-cone transcranial magnetic stimulation
IntroductionThe double-cone coil (D-CONE) is frequently used in transcranial magnetic stimulation (TMS) experiments that target the motor cortex (M1)...
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Organ-specific off-target effects of Pim/ZIP kinase inhibitors suggest lack of contractile Pim kinase activity in prostate, bladder, and vascular smooth muscle
Smooth muscle contraction by Pim kinases and ZIPK has been suggested, but evidence for lower urinary tract organs or using Pim-selective inhibitor...
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In vivo cytidine base editing of hepatocytes without detectable off-target mutations in RNA and DNA
Base editors are RNA-programmable deaminases that enable precise single-base conversions in genomic DNA. However, off-target activity is a concern in...
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Using RNA-seq to Assess Off-Target Effects of Antisense Oligonucleotides in Human Cell Lines
BackgroundThe field of antisense oligonucleotide therapeutics is rapidly growing and in addition to small molecules and therapeutic antibodies,...
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Selection of Chemical Modifications in the siRNA Seed Region That Repress Off-Target Effect
RNA interference mediated by small interfering RNA (siRNA) has been widely used as a procedure to knock down the expression of an intended target...
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Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individuals
BackgroundRecent studies suggested the existence of (poly-)microbial infections in human brains. These have been described either as putative...
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Improved analysis of CRISPR fitness screens and reduced off-target effects with the BAGEL2 gene essentiality classifier
BackgroundIdentifying essential genes in genome-wide loss-of-function screens is a critical step in functional genomics and cancer target finding. We...
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Mitigating off-target distribution and enhancing cytotoxicity in breast cancer cells with alpha-ketoglutaric acid-modified Fe/Mg-CA nanoparticles
PurposeIn this work, pH-sensitive alpha-ketoglutaric acid-modified Fe/Mg-carbonate apatite (α-KAM-Fe/Mg-CA) NPs were introduced and found to be...
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Mitigating off-target effects in CRISPR/Cas9-mediated in vivo gene editing
The rapid advancement of genome editing technologies has opened up new possibilities in the field of medicine. Nuclease-based techniques such as the...
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Do Fasting, CR and FMD Improve the Chemotherapy Response, Reduce Off-Target Toxicities and Enhance Antitumour Immunity? Illusion or Clinical Reality?
Besides the great strides of efforts made towards understanding the effect of fasting, calorie restriction (CR) and fasting-mimicking diet (FMD), yet...
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Structure-guided discovery of highly efficient cytidine deaminases with sequence-context independence
The applicability of cytosine base editors is hindered by their dependence on sequence context and by off-target effects. Here, by using AlphaFold2...
