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Nanobody-Targeted Photodynamic Therapy : Nanobody Production and Purification
Nanobodies have recently been introduced to the field of photodynamic therapy (PDT)Photodynamic therapy (PDT) as a very promising strategy to target...
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Extreme thermal stability of the antiGFP nanobody – GFP complex
ObjectiveThe green fluorescent protein (GFP) and its derivatives are widely used in biomedical research. The manipulation of GFP-tagged proteins by...
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Enhanced in vivo blood brain barrier transcytosis of macromolecular cargo using an engineered pH-sensitive mouse transferrin receptor binding nanobody
BackgroundThe blood brain barrier limits entry of macromolecular diagnostic and therapeutic cargos. Blood brain barrier transcytosis via receptor...
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Preventing SARS-CoV-2 Infection Using Anti-spike Nanobody-IFN-β Conjugated Exosomes
PurposeTo inhibit the transmission of SARS-CoV-2, we developed engineered exosomes that were conjugated with anti-spike nanobodies and type I...
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Tumour inhibitory activity on pancreatic cancer by bispecific nanobody targeting PD-L1 and CXCR4
Background:Antibodies and derivative drugs targeting immune checkpoints have been approved for the treatment of several malignancies, but there are...
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Engineering Nanobody Targeting Cancer Stem Cells
Nanobodies (Nbs) are great molecular tools that can circumvent the limitations of traditional antibodies such as large size, low stability, slow...
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Nanobody-based CAR-T cells for cancer immunotherapy
Chimeric antigen receptor T-cell (CAR-T) therapy is the result of combining genetic engineering-based cancer immunotherapy with adoptive cell therapy...
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In Vitro Assessment of Binding Affinity, Selectivity, Uptake, Intracellular Degradation, and Toxicity of Nanobody-Photosensitizer Conjugates
Photosensitizers have recently been conjugated to nanobodies for targeted photodynamic therapy (PDT) to selectively kill cancer cells. The success of...
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Structure-based design and construction of a synthetic phage display nanobody library
ObjectiveTo design and construct a new synthetic nanobody library using a structure-based approach that seeks to maintain high protein stability and...
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Construction of stable membranal CMTM6-PD-L1 full-length complex to evaluate the PD-1/PD-L1-CMTM6 interaction and develop anti-tumor anti-CMTM6 nanobody
CKLF (chemokine-like factor)-MARVEL transmembrane domain containing protein 6 (CMTM6) is a novel regulator to maintain the stability of PD-L1. CMTM6...
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Assessment of the In Vivo Response to Nanobody-Targeted PDT Through Intravital Microscopy
Methods that allow real-time, longitudinal, intravital detection of the fluorescence distribution and the cellular and vascular responses within...
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Detection of HER2 expression using 99mTc-NM-02 nanobody in patients with breast cancer: a non-randomized, non-blinded clinical trial
Background99m Tc radiolabeled nanobody NM-02 ( 99m Tc-NM-02) is a novel single photon emission computed tomography (SPECT) probe with a high affinity...
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Orthotopic Breast Cancer Model to Investigate the Therapeutic Efficacy of Nanobody-Targeted Photodynamic Therapy
Photodynamic therapy (PDT) is characterized by the local application of laser light, which activates a photosensitizer to lead to the formation of...
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Generation and Characterization of a Nanobody Against SARS-CoV
The sudden emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) has caused global panic in 2003, and the risk of SARS-CoV outbreak...
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Investigation of the Therapeutic Potential of Nanobody-Targeted Photodynamic Therapy in an Orthotopic Head and Neck Cancer Model
Photodynamic therapy (PDT) has a great therapeutic potential because it induces local cellular cytotoxicity upon application of a laser light that...
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Rapid single-molecule detection of COVID-19 and MERS antigens via nanobody-functionalized organic electrochemical transistors
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the need for rapid and sensitive protein detection and quantification in simple and...
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Nanobody-armed T cells endow CAR-T cells with cytotoxicity against lymphoma cells
BackgroundTaking advantage of nanobodies (Nbs) in immunotherapy, we investigated the cytotoxicity of Nb-based chimeric antigen receptor T cells (Nb...
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Development of nanobodies targeting hepatocellular carcinoma and application of nanobody-based CAR-T technology
BackgroundChimeric antigen receptor T (CAR-T) cell therapy, as an emerging anti-tumor treatment, has garnered extensive attention in the study of...
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Induction of antigen-specific tolerance by nanobody–antigen adducts that target class-II major histocompatibility complexes
The association of autoimmune diseases with particular allellic products of the class-II major histocompatibility complex (MHCII) region implicates...
