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In Situ Hybridization of Brain Slices
In situ hybridization (ISH) is an important technique for identifying gene expression at the cellular level in various organs, including brain...
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Affordable optical clearing and immunolabelling in mouse brain slices
Traditional histological analysis is conducted on thin tissue sections, limiting the data capture from large tissue volumes to 2D profiles, and...
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Electrophysiological Recordings of Oligodendroglia in Adult Mouse Brain Slices
Communication between neurons and oligodendrocyte lineage cells has attracted a great interest since multiple discoveries revealed its important...
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APOE4 genotype and aging impair injury-induced microglial behavior in brain slices, including toward Aβ, through P2RY12
Microglia are highly dynamic cells that play a critical role in tissue homeostasis through the surveillance of brain parenchyma and response to cues...
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Characterizing Dopamine Transporter Mediated Uptake and Efflux in Brain Slices: A New Approach
The dopamine transporter (DAT) plays a fundamental role in dopamine (DA) neurotransmission by retrieving the transmitter from the extracellular space...
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Monoacylglycerol Lipase Inhibition Prevents Short-Term Mitochondrial Dysfunction and Oxidative Damage in Rat Brain Synaptosomal/Mitochondrial Fractions and Cortical Slices: Role of Cannabinoid Receptors
Inhibition of enzymes responsible for endocannabinoid hydrolysis represents an invaluable emerging tool for the potential treatment of...
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A Mechanism of Action of Metformin in the Brain: Prevention of Methylglyoxal-Induced Glutamatergic Impairment in Acute Hippocampal Slices
Metformin, a biguanide compound (N-1,1-dimethylbiguanide), is widely prescribed for diabetes mellitus type 2 (T2D) treatment. It also presents a...
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Functional Analysis of NMDAR Subunit Components in Postsynaptic Currents of Identified Cells and Synapses in Brain Slices
Epilepsy is one of the most represented neurological diseases worldwide. However, in many cases, the precise molecular mechanisms of epileptogenesis...
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Protocol to Study West Nile Virus Infection in Brain Slices In Vitro
Ex vivo slice cultures of the brain tissue can maintain the cytoarchitecture of the central nervous system (CNS), which allows a thorough...
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Utilization of Superfused Cerebral Slices in Probing Muscarinic Receptor Autoregulation of Acetylcholine Release
Signal transmission from cholinergic nerves is mediated by two classes of receptors: (1) ionotropic nicotinic receptor channels and (2) metabotropic...
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Neonatal Isoflurane Exposure in Rats Impairs Short-Term Memory, Cell Viability, and Glutamate Uptake in Slices of the Frontal Cerebral Cortex, But Not the Hippocampus, in Adulthood
Neonatal exposure to general anesthetics has been associated with neurotoxicity and morphologic changes in the develo** brain. Isoflurane is a...
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Comparative analysis of spreading depolarizations in brain slices exposed to osmotic or metabolic stress
BackgroundRecurrent spreading depolarizations (SDs) occur in stroke and traumatic brain injury and are considered as a hallmark of injury...
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Metabolic Changes in Brain Slices over Time: a Multiplatform Metabolomics Approach
Brain slice preparations are widely used for research in neuroscience. However, a high-quality preparation is essential and there is no consensus...
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Homocysteine May Decrease Glucose Uptake and Alter the Akt/GSK3β/GLUT1 Signaling Pathway in Hippocampal Slices: Neuroprotective Effects of Rivastigmine and Ibuprofen
Homocysteine (Hcy) is a risk factor for neurodegenerative diseases, such as Alzheimer’s Disease, and is related to cellular and tissue damage. In the...
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Localization and Registration of 2D Histological Mouse Brain Images in 3D Atlas Space
To accurately explore the anatomical organization of neural circuits in the brain, it is crucial to map the experimental brain data onto a...
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Phoenixin-14 reduces the frequency of interictal-like events in mice brain slices
Phoenixin-14 (PNX-14) has a wide bioactivity in the central nervous system. Its role in the hypothalamus has been investigated, and it has been...
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Human post-mortem organotypic brain slice cultures: a tool to study pathomechanisms and test therapies
Human brain experimental models recapitulating age- and disease-related characteristics are lacking. There is urgent need for human-specific tools...
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Herpes simplex virus infection induces necroptosis of neurons and astrocytes in human fetal organotypic brain slice cultures
BackgroundHerpes simplex virus (HSV) encephalitis (HSE) is a serious and potentially life-threatening disease, affecting both adults and newborns....
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β-Hydroxybutyrate and Medium-Chain Fatty Acids are Metabolized by Different Cell Types in Mouse Cerebral Cortex Slices
Ketogenic diets and medium-chain triglycerides are gaining attention as treatment of neurological disorders. Their major metabolites,...
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Immunohistochemistry of Brain Tissues
Immunohistochemistry (IHC) is the basis of histological or pathological analysis and is widely used to enable the detection and characterization of...