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Simultaneous detection of influenza A, B and respiratory syncytial virus in wastewater samples by one-step multiplex RT-ddPCR assay
BackgroundAfter the occurrence of the COVID-19 pandemic, detection of other disseminated respiratory viruses using highly sensitive molecular methods...
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The Green Synthesis of Carbon Quantum Dots through One-step Hydrothermal Approach by Orange Juice for Rapid, and Accurate Detection of Dopamine
In the current study, the fluorescent Carbon quantum dots (CDs) were synthesized through one-step hydrothermal approach by orange juice without any...
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Single Step Solid State Synthesis of Carbon Nanoparticles for Instantaneous Detection of Fe (III) in Water Samples
Though iron is one of the vital micronutrients in biological systems excess of which is associated with various illness. Consumption of contaminated...
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Detection and ty** of dengue virus by one-step RT-PCR-based high-resolution melting assay
Dengue is a rapidly spreading arboviral disease that can be transmitted through any of the four types of dengue virus (DENV) serotypes. Previous...
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One-step SYBR green-based real-time RT-PCR assay for detection of foot-and-mouth disease virus circulating in India
Rapid, sensitive, and reliable laboratory detection of foot-and-mouth disease virus (FMDV) infection is essential for containing and controlling...
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A simple preparation step to remove excess liquid lipids in white adipose tissue enabling improved detection of metabolites via MALDI-FTICR imaging MS
Matrix-assisted laser desorption ionization (MALDI) Fourier transform ion cyclotron resonance (FTICR) imaging mass spectrometry (MS) is a powerful...
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Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses
Coronavirus disease 2019 (COVID-19) is an acute respiratory infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Other...
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DNA Modification Detection Methods
Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and map** of DNA modifications. Methods...
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A Dual-Mode Detection Sensor Based on Nitrogen-Doped Carbon Dots for Visual Detection of Fe(III) and Ascorbic Acid via a Smartphone
Accurately and promptly detecting Fe 3+ and ascorbic acid (AA) is a crucial objective. In this study, nitrogen-doped carbon dots (N-CDs) were...
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Nuclei detection in breast histopathology images with iterative correction
This work presents a deep network architecture to improve nuclei detection performance and achieve the high localization accuracy of nuclei in breast...
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Multi-resolution auto-encoder for anomaly detection of retinal imaging
Identifying unknown types of diseases is a crucial step in preceding retinal imaging classification for the sake of safety, which is known as anomaly...
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Evidence for the circulation of genotype 4 Eurasian avian-like lineage swine H1N1 influenza A viruses on Korean swine farms, obtained using a newly developed one-step multiplex RT-qPCR assay
Genotype 4 (G4) Eurasian avian-like lineage swine H1N1 influenza A viruses, which are reassortants containing sequences from the pandemic 2009 H1N1...
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A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs
The use of microRNAs as clinical cancer biomarkers is hindered by the absence of accurate, fast and inexpensive assays for their detection in...
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One step synthesis of ultra-high quantum yield fluorescent carbon dots for “on-off-on” detection of Hg2+ and biothiols
In this paper, the carbon dots (CDs) with strong blue fluorescence were synthesized through hydrothermal method, which using folic acid, ammonium...
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One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Droplet digital polymerase chain reaction (ddPCR) is a third generation of PCR that was recently developed to overcome the limitation of direct...
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SARS-CoV-2 Detection and Genome Sequencing in Urban Wastewaters
Due to the excretion of SARS-CoV-2 in faeces, the use of wastewater-based epidemiology (WBE) is a useful tool for virus surveillance in large...
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Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients
Mitochondria-associated membranes (MAMs) are regions where the endoplasmic reticulum (ER) interacts with mitochondria and regulate lipid trafficking,...
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Rapid differential detection of subtype H1 and H3 swine influenza viruses using a TaqMan-MGB-based duplex one-step real-time RT-PCR assay
Swine influenza is an economically important respiratory disease in swine, but it also constantly poses a threat to human health. Therefore,...
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Step by Step Toward an Amyloid Beta Peptide-Based Hypothesis of Alzheimer’s Disease
Following the Alzheimer’s research decisive year 1987, the compass was set toward further exploration of the Amyloid–Alzheimer’s link. A great amount...
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Meta-analysis of the quantitative assessment of lower extremity motor function in elderly individuals based on objective detection
ObjectiveTo avoid deviation caused by the traditional scale method, the present study explored the accuracy, advantages, and disadvantages of...
