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Characterization of Native COMPASS Complex in Urothelial Carcinoma Cells by Size Exclusion Chromatography
The human COMPASS complexes regulate gene expression during development and cell differentiation. Three distinct subunits, KMT2C, KMT2D, and KDM6A...
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Confirming size-exclusion chromatography as a clinically relevant extracellular vesicles separation method from 1mL plasma through a comprehensive comparison of methods
BackgroundExtracellular vesicles (EVs) are amongst the most promising candidates for develo** blood-based biomarkers. However, patient sample...
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Analysis of Host Cell Proteins in Monoclonal Antibody Therapeutics Through Size Exclusion Chromatography
PurposeHost cell proteins (HCPs) are impurities derived from expression systems during the manufacturing of biotherapeutics. Even trace amounts of...
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Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery
Structural discovery of viral chemokine binding proteins can provide valuable information on the binding domains and protein–protein interfaces (PPI)...
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Rapid Isolation of Functional Synaptic Vesicles from Tissues Through Cryogrinding, Ultracentrifugation, and Size Exclusion Chromatography
Many biochemical and biophysical related questions require the isolation of functional synaptic vesicles. Isolated synaptic vesicles can be used for...
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Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma
BackgroundExtracellular vesicles (EVs), known as cell-derived membranous structures harboring a variety of biomolecules, have been widely used in...
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Gel Filtration Chromatography
Apart from finding multitudes of applications in chemical, medicinal, and pharmaceutical research, gel filtration chromatography (GFC) has also...
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Purifying Viral Vectors: A Review of Chromatography Solutions
The use of viral vectors in the production of biotherapeutics continues to rise globally. As clinical applications expand, there is an increased need...
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Protein Purification by Ion Exchange Chromatography
Separation of similar biomolecules and proteins with little or no differences in molecular weight or without tags can be difficult with...
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A Rapid Exosome Isolation Using Ultrafiltration and Size Exclusion Chromatography (REIUS) Method for Exosome Isolation from Melanoma Cell Lines
Cells release extracellular vesicles (EVs) that can be detected both in vivo and in cell culture medium. Among EVs, exosomes are 50–150 nm vesicles...
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Expression and Purification of Mammalian NMDA Receptor Protein for Functional Characterization
N-methyl-D-aspartate (NMDA) receptors are critical for brain function and serve as drug targets for the treatment of neurological and psychiatric...
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Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles
BackgroundCirculating small extracellular vesicles (sEVs) and its associated proteins are of great interest in the early detection of many diseases....
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Determination of N6-Methyladenine in DNA of Mammals and Plants by Dpn I Digestion Combined with Size-Exclusion Ultrafiltration and Mass Spectrometry Analysis
DNA adenine methylation (N6-methyladenine, 6mA) widely exists in bacteria and regulates various biological processes including gene transcription and...
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Methods for the Isolation of Extracellular Vesicles
Purification of extracellular vesicles (EVs) from different sample types is a critical step that is performed prior to biological and clinical...
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Purification of Plasmid DNA by Multimodal Chromatography
Multimodal (MM) chromatography can be described as a chromatographic method that uses more than one mode of interaction between the target molecule...
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Chromatography
The successful chromatographic separation of analytes in a mixture depends upon the selection of the most appropriate process of chromatography...
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Quantitation of Active Pharmaceutical Ingredients in Polymeric Drug Products Using Elemental Analysis
Polymeric prodrugs have gained significant popularity as a strategy to enhance the bioavailability and improve the pharmacokinetic properties of...
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Progress in mass spectrometry approaches to profiling protein–protein interactions in the studies of the innate immune system
Understanding protein–protein interactions (PPIs) is pivotal for deciphering the intricacies of biological processes. Dysregulation of PPIs underlies...
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Falsified and problematic methandienone products available online: active pharmaceutical ingredient identification by portable Raman spectrometers and quantification by ultra-high-performance liquid chromatography–Fourier transform mass spectrometry
This study aimed on the one hand to clarify the quality, authenticity, safety, and other issues related to products of the anabolic-androgenic...
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Role of Charge Heterogeneity on Physical Stability of Monoclonal Antibody Biotherapeutic Products
PurposeChemical modifications in monoclonal antibodies can change hydrophobicity, charge heterogeneity as well as conformation, which eventually can...
