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Utility of I-SceI and CCR5-ZFN nucleases in excising selectable marker genes from transgenic plants
ObjectivesRemoval of selection marker genes from transgenic plants is highly desirable for their regulatory approval and public acceptance. This...
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Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica
Pathway localization by fluorophore or epitope tagging can be accomplished through a multi-staged DNA construct and confirmation process, to generate...
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Genome Engineering of Yarrowia lipolytica with the PiggyBac Transposon System
A mutant excision+/integrationâ piggyBac transposase can be used to seamlessly excise a chromosomally integrated, piggyBac-compatible selection...
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Obtaining Wheat (Triticum aestivum L.) Lines with Yeast Genes for Trehalose Biosynthesis
AbstractYeast ( Saccharomyces cerevisiae ) genes for trehalose biosynthesis ( TPS1 a nd TPS2 ) were transferred into genomes of several common wheat...
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Using Bakerâs Yeast to Determine Functions of Novel Wolbachia (and Other Prokaryotic) Effectors
Yeasts are single-celled eukaryotic organisms classified as fungi, mostly in the phylum Ascomycota. Of about 1500 named species, Saccharomyces...
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Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting
BackgroundRecombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swap** Method...
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Enrichment strategies to enhance genome editing
Genome editing technologies hold great promise for numerous applications including the understanding of cellular and disease mechanisms and the...
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Stable transfection system for Babesia sp. **njiang
BackgroundStable transfection systems have been described in many protozoan parasites, including Plasmodium falciparum , Cryptosporidium parvum , Babesi...
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Detection and Quantification of Conjugative Transfer of Mobile Genetic Elements Carrying Antibiotic Resistance Genes
Multidrug resistance, due to acquired antimicrobial resistance genes, is increasingly reported in the zoonotic pathogen Streptococcus suis. Most of...
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CRISPR/Cas9 Editing of the Plasmodium falciparum Genome
The ability to interrogate gene function in Plasmodium parasites has been greatly enhanced by the advent of CRISPR/Cas9 systems. The breadth of...
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Constructional Features of a Multielectrode Electrocardiology Screening System
The challenges of constructing a noninvasive screening system for electrocardiodiagnostics, focused on visualization of electric potential maps on...
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A Brief Introduction to Recombinant DNA Technology
The discovery of plasmid DNA in the early 1950s marks the beginning of recombinant DNA (rDNA) technology although there remained a big hiatus between...
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Use of Drosophila Transgenics to Identify Functions for Symbiont Effectors
Wolbachia, one of the most successful and studied insect symbionts, and Drosophila, one of the most understood model insects, can be exploited as...
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Let it glow: genetically encoded fluorescent reporters in Plasmodium
The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this obligate intracellular protozoon. FPs...
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Creation and preclinical evaluation of genetically attenuated malaria parasites arresting growth late in the liver
Whole-sporozoite (WSp) malaria vaccines induce protective immune responses in animal malaria models and in humans. A recent clinical trial with a WSp...
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Life-history traits of a fluorescent Anopheles arabiensis genetic sexing strain introgressed into South African genomic background
BackgroundSouth Africa has set a mandate to eliminate local malaria transmission by 2023. In pursuit of this objective a Sterile Insect Technique...
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Selection of Cloning and Expression Plasmid Vectors
Purification of proteins using recombinant DNA technology is crucial for studying its structure, function, and interactions with other...
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Heterologous Expression of Candida Antifungal Target Genes in the Model Organism Saccharomyces cerevisiae
Understanding how a gene variant may influence antifungal resistance, or other phenotypic characteristics, is an important step in determining or...