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Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
Protein, being a fundamental biological macromolecule, assumes a critical function in various vital activities of cellular growth, heredity,...
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Production and Purification of Recombinant Proteins
The growing demand for therapeutic proteins, especially monoclonal antibodies, increases the need for practical and economical processing techniques....
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Recombinant AAV Production
The insect cell-baculovirus expression vector (IC-BEV) platform has enabled small research-scale and large commercial-scale production of recombinant...
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Recombinant Coagulation Factors and Thrombolytic Agents
Bleeding disorders and thrombosis are defects of the hemostatic system that often require therapeutic intervention with human plasma proteins....
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Construction of Recombinant Baculoviruses
The success of using the insect cell-baculovirus expression technology (BEST) relies on the efficient construction of recombinant baculovirus with...
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Textbook on Cloning, Expression and Purification of Recombinant Proteins
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA...
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Evaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model
BackgroundBaylisascaris schroederi is the most common and harmful intestinal parasitic nematode of giant pandas, causing ascariasis. Although drug...
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Construction of and evaluation of the immune response to two recombinant pseudorabies viruses expressing the B119L and EP364R proteins of African swine fever virus
African swine fever (ASF) is an infectious disease caused by ASF virus (ASFV), which is characterized by high infectivity, rapid onset of disease,...
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Production of recombinant HPV11/16 E6/E7-MBP-His6 fusion proteins and their potential to induce cytokine secretion by immune cells in peripheral blood
Human papillomavirus (HPV) infection poses a significant threat to public health worldwide. Targeting the function of HPV E6 and E7 proteins and...
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Recombinant Production of Food Allergens in Yeast Pichia pastoris
The methylotrophic yeast Pichia pastoris (P. pastoris) is one of the expression systems widely used to produce recombinant heterologous proteins. In...
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Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System
Mammalian cell lines are one of the best options when it comes to the production of complex proteins requiring specific glycosylation patterns....
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Recovery of Recombinant Rotaviruses by Reverse Genetics
Rotaviruses are the primary cause of severe gastroenteritis in infants and young children throughout the world. To combat rotavirus illness, several...
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Nonviral Platform for Expression of Recombinant Protein in Insect Cells
Nonviral transfection has been used to express various recombinant proteins, therapeutics, and virus-like particles (VLP) in mammalian and insect...
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Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes
Reverse genetics allows for the generation of recombinant infectious viruses from viral sequences or complete viral genomes cloned into plasmids....
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Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI
BackgroundAfrican swine fever (ASF) is a highly fatal swine disease, which threatens the global pig industry. There is no commercially available...
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Recombinant Soluble Henipavirus Glycoprotein Preparation
Henipaviruses possess two envelope glycoproteins, the attachment (G) and the fusion (F) proteins that mediate cellular entry and are the major...
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Reactivity of DENV-positive sera against recombinant envelope proteins produced in bacteria and eukaryotic cells
Dengue is a mosquito-borne disease endemic in many tropical and subtropical countries. It is caused by the dengue virus (DENV) that can be classified...
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Immunogenicity of Therapeutic Proteins
The use of proteins for medical applications has revolutionized therapies of many diseases/disorders that were often incurable with small-molecule...
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Generation of Complex Protein Structures by Coinfection with High-Quality Recombinant Baculovirus
The baculovirus expression vector system (BEVSbaculovirus expression vector system (BEVS))Baculovirus expression vector system (BEVS) is a powerful...