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Comparative performance of microscopy, nested PCR, and real-time PCR for screening avian haemosporidian parasites in Afrotropical starlings (family Sturnidae)
Prevalence studies of avian haemosporidian parasites frequently use microscopy and the nested polymerase chain reaction (PCR) protocols for detecting...
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Development of Real-Time PCR Kits for Bovine Haplotypes HH3, HH6, HH7 Diagnosis
AbstractDairy cattle breeding is aimed at improving the productivity, mainly through the use of a limited number of breeding bulls. As a result, an...
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Evaluation of different genomic regions of rotavirus B and rotavirus C for development of real-time RT‒PCR assays
BackgroundThe causative agents of diarrhea, rotavirus B (RVB) and rotavirus C (RVC) are common in adults and patients of all age groups,...
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Determination for KIR genotype and allele copy number via real-time quantitative PCR method
Killer cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) play crucial roles in regulating NK cell activity. Here, we report a...
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Intramolecular Interactions in the Fluorophore–Quencher System in Linear and Hairpin Probes for Real-Time PCR
AbstractThe hairpin probe is characterized by a higher fluorescence quenching efficiency as compared with the linear one under the conditions of...
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Adaptation of the classical end-point ITS-PCR for the diagnosis of avian trichomonosis to a real-time PCR reveals Bonelli’s eagle as a new host for Trichomonas gypaetinii
Avian trichomonosis is a parasitic disease caused mainly by Trichomonas gallinae and other Trichomonas species. It can be asymptomatic, or it can...
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Detection of rat lungworm (Angiostrongylus cantonensis) infection by real-time PCR from the peripheral blood of animals: a preliminary study
Rat lungworm disease or neuroangiostrongyliasis is a cerebral parasitic infection that affects humans and animals alike. Its clinical signs and...
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Real-Time RT-PCR for Detection of Feline Coronavirus
Feline coronavirus (FCoV) is an enveloped single-stranded positive-sense RNA virus. FCoV consists of two different types: the ubiquitous enteric...
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Real-Time PCR Assays on Formalin-Fixed, Paraffin-Embedded Medulloblastomas
Real-time PCR technology has been instrumental in contributing toward biomarker discovery, classification of tumors as well as risk stratification of...
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Identification of Differentially Expressed Genes in Human Colorectal Cancer Using RNASeq Data Validated on the Molecular Level with Real-Time PCR
Colorectal cancer (CRC) is a prevalent cancer with high morbidity and mortality rates worldwide. Late diagnosis is a significant contributor to low...
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Quantitative Real-Time RT-PCR Systems to Detect SARS-CoV-2
Since the outbreak of coronavirus disease 2019 (COVID-19) on the Diamond Princess cruise ship docked at Yokohama Port on February 3, 2020, real-time...
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TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus
Porcine epidemic diarrhea virus (PEDV) is a highly infectious agent that causes the fatal enteric disease porcine epidemic diarrhea (PED) in neonatal...
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A multiplex real-time RT-PCR assay for the detection of H1, H2 and H3 subtype avian influenza viruses
Avian influenza viruses (AIVs) are influenza A viruses, of which subtypes H1, H2 and H3 are highly transmissible in poultry and have the risk of...
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Monitoring of HTLV-1-associated diseases by proviral load quantification using multiplex real-time PCR
Proviral load (PVL) is one of the determining factors for the pathogenesis and clinical progression of the human T-lymphotropic virus type I (HTLV-1)...
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Diagnostic performance of mpox virus (MPXV) real-time PCR assays: multicenter assessment and extended sensitivity analysis
PurposeTo comprehensively investigate the diagnostic performance of routinely used assays in MPXV testing, the National Center of Clinical...
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Viral Quantification in Bee Samples Using Synthetic DNA Sequences with Real-Time PCR (qPCR)
Pathogen spillover between honey bees and wild pollinators is a relatively new and exciting field of study. It is known that some viral diseases are...
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Molecular detection of Nocardia: development and application of a real-time PCR assay in sputum and bronchoalveolar lavage fluid samples
The diagnosis of pulmonary nocardiosis remains challenging. Rapid detection of Nocardia is of primary importance for early diagnosis and precise...
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SARS-CoV-2 Real-Time RT-PCR Assay in Animals
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped non-segmented, single-stranded, positive-sense RNA virus. SARS-CoV-2 was...
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Development of a novel sequence based real-time PCR assay for specific and sensitive detection of Burkholderia pseudomallei in clinical and environmental matrices
BackgroundMelioidosis, caused by the category B biothreat agent Burkholderia pseudomallei , is a disease with a high mortality rate and requires an...
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Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
BackgroundParechoviruses (PeV-As), which constitute a new genus within the family Picornaviridae , have been associated with numerous localized...
