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RNAscope in situ hybridization reveals microvascular sequestration of Plasmodium relictum pSGS1 blood stages but absence of exo-erythrocytic dormant stages during latent infection of Serinus canaria
BackgroundBirds chronically infected with avian malaria parasites often show relapses of parasitaemia after latent stages marked by absence of...
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Evaluation of the Suitability of RNAscope as a Technique to Measure Gene Expression in Clinical Diagnostics: A Systematic Review
ObjectiveTo evaluate the application of RNAscope in the clinical diagnostic field compared to the current ‘gold standard’ methods employed for...
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An Efficient Method to Detect Messenger RNA (mRNA) in the Inner Ear by RNAscope In Situ Hybridization
Biological processes are largely governed by the RNA molecules and resulting peptides that are encoded by an organism’s DNA. For decades, our...
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In situ HER2 RNA expression as a predictor of pathologic complete response of HER2-positive breast cancer patients receiving neoadjuvant chemotherapy and anti-HER2 targeted treatment
BackgroundImmunohistochemistry (IHC) and in situ hybridization (ISH) remain standard biomarkers for therapeutic decisions in human epidermal growth...
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Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and...
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RNAscope dual ISH–IHC technology to study angiogenesis in diffuse large B-cell lymphomas
Diffuse large B-cell lymphomas (DLBCLs) are the most common types of Non-Hodgkin’s lymphomas and are highly heterogeneous in terms of phenotype and...
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Expression patterns of Piezo1 in the develo** mouse forebrain
Malformation during cortical development can disrupt the balance of excitatory and inhibitory neural circuits, contributing to various psychiatric...
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Transmitted/founder SHIV.D replicates in the brain, causes neuropathogenesis, and persists on combination antiretroviral therapy in rhesus macaques
A biologically relevant non-human primate (NHP) model of HIV persistence in the central nervous system (CNS) is necessary. Most current NHP/SIV...
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Human papillomavirus E6E7 mRNA and TERC lncRNA in situ detection in cervical scraped cells and cervical disease progression assessment
BackgroundHuman papillomavirus screen in female cervical cells has demonstrated values in clinical diagnosis of precancerous lesions and cervical...
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Localization of natriuretic peptide receptors A, B, and C in healthy and diseased mouse kidneys
The natriuretic peptides (NPs) ANP (atrial natriuretic peptide) and BNP (B-type natriuretic peptide) mediate their widespread effects by activating...
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HPV E6/E7 mRNA in situ hybridization in endocervical adenocarcinoma: implications for prognosis and diagnosis
BackgroundHuman papillomavirus (HPV) E6/E7 mRNA in situ hybridization (HPV E6/E7 RNAscope) appears to be a sensitive and specific technique in...
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The glycine receptor alpha 3 subunit mRNA expression shows sex-dependent differences in the adult mouse brain
BackgroundThe glycinergic system plays an important inhibitory role in the mouse central nervous system, where glycine controls the excitability of...
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EBV persistence in gastric cancer cases conventionally classified as EBER-ISH negative
BackgroundThe Epstein-Barr virus (EBV) causes various B-cell lymphomas and epithelial malignancies, including gastric cancer (GC) at frequencies...
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Abcg2a is the functional homolog of human ABCG2 expressed at the zebrafish blood–brain barrier
BackgroundA principal protective component of the mammalian blood–brain barrier (BBB) is the high expression of the multidrug efflux transporters...
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Reliable detection of RNA in hippocampus sections of mice by FISH up to a post-mortem delay of 24 h
Proteins can be successfully localized in post-mortem (PM) brain tissue sections if the time until PM tissue sampling is not too long. In this study,...
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Axo-glial interactions between midbrain dopamine neurons and oligodendrocyte lineage cells in the anterior corpus callosum
Oligodendrocyte progenitor cells (OPCs) receive synaptic innervation from glutamatergic and GABAergic axons and can be dynamically regulated by...
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Microglia proliferation underlies synaptic dysfunction in the prefrontal cortex: implications for the pathogenesis of HIV-1-associated neurocognitive and affective alterations
Microglia, which are productively infected by HIV-1, are critical for brain development and maturation, as well as synaptic plasticity. The...
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Modeling HIV-1 infection and NeuroHIV in hiPSCs-derived cerebral organoid cultures
The human immunodeficiency virus (HIV) epidemic is an ongoing global health problem affecting 38 million people worldwide with nearly 1.6 million new...
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Neuronal SNCA transcription during Lewy body formation
Misfolded α-synuclein (α-syn) is believed to contribute to neurodegeneration in Lewy body disease (LBD) based on considerable evidence including a...
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α-Fetoprotein mRNA in situ hybridisation is a highly specific marker of hepatocellular carcinoma: a multi-centre study
BackgroundPathologic diagnosis of hepatocellular carcinoma (HCC) can be challenging in differentiating from benign and non-hepatocytic malignancy...
