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Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method
PurposeThis study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR)...
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Polymerase Chain Reaction: An Indispensable Molecular Biology Tool of 21st Century
Due to its universal applications, Polymerase Chain Reaction (PCR) became an essential technique in various scientific branches such as microbiology,...
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Multiplex Quantitative Polymerase Chain Reaction Test to Identify SARS-CoV-2 Variants
Quantitative polymerase chain reaction (qPCR) is a routinely used method for detection and quantitation of gene expression in real time. This is...
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Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses
COVID-19 disease caused by the novel SARS-CoV-2 virus represents a new challenge for healthcare systems. The molecular confirmation of infection is...
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A multiplex real-time reverse transcription polymerase chain reaction assay for differentiation of classical and variant II strains of avian infectious bronchitis virus
Identification of avian infectious bronchitis virus (IBV) genotypes is essential for controlling infectious bronchitis (IB) disease, because vaccines...
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First report on knockdown resistance mutations in wild populations of Aedes aegypti from Argentina determined by a novel multiplex high-resolution melting polymerase chain reaction method
BackgroundThe mosquito Aedes aegypti is an urban vector of dengue and other arboviruses. During epidemics of these viruses, pyrethroid insecticides...
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Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods
Leishmania infections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world’s poorest regions....
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Magnetic-bead-based DNA-capture-assisted real-time polymerase chain reaction and recombinase polymerase amplification for the detection of African swine fever virus
African swine fever (ASF) is a deadly disease in swine caused by African swine fever virus (ASFV). The global spread of ASFV has resulted in...
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Multiplex PCR for Diagnosis of Porcine Diseases
Molecular diagnostics have revolutionized the efficiency of diagnosis of infectious diseases. An accurate diagnosis of a disease would prevent...
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Comparison of a multiplex PCR with DNA barcoding for identification of container breeding mosquito species
BackgroundIdentification of mosquitoes greatly relies on morphological specification. Since some species cannot be distinguished reliably by...
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One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y
BackgroundCertification of seed potato as free of viruses is essential for stable potato production. Among more than 30 virus species infecting...
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Multiplex PCR Design for Scalable Resequencing
While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable...
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Clinical factors influencing the performance of bacterial multiplex polymerase chain reaction in patients with community-onset pneumonia
The etiologic diagnostic yield of community-onset pneumonia (COP) using conventional methods is low. Bacterial multiplex polymerase chain reaction...
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Development of a multiplex RT-PCR assay for simultaneous detection of Lily symptomless virus, Lily mottle virus, Cucumber mosaic virus, and Plantago asiatica mosaic virus in Lilies
BackgroundViral pathogens causing significant economic losses in lilies ( Lilium spp. and hybrids) include Lily symptomless virus (LSV), Lily mottle...
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Herpesvirus encephalitis diagnosed by polymerase chain reaction at the National Institute of Neurology of Mexico
The frequency of central nervous system infections due to herpesvirus have been studied in various populations; however, studies in Mexican mestizo...
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Genetic analysis challenges the presence of Ixodes inopinatus in Central Europe: development of a multiplex PCR to distinguish I. inopinatus from I. ricinus
BackgroundIxodes ricinus is an important vector of several pathogens, primarily in Europe. Recently, Ixodes inopinatus was described from Spain,...
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A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic
A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify...
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Simultaneous detection of influenza A, B and respiratory syncytial virus in wastewater samples by one-step multiplex RT-ddPCR assay
BackgroundAfter the occurrence of the COVID-19 pandemic, detection of other disseminated respiratory viruses using highly sensitive molecular methods...
