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Lobetyolin, a Q-marker isolated from Radix Platycodi, exerts protective effects on cisplatin-induced cytotoxicity in HEK293 cells
This study investigated the protective effect of lobetyolin (LBT), a Q-marker isolated from the roots of Platycodon grandiflorum (Radix Platycodi),...
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Isolation of human TRPA1 channel from transfected HEK293 cells and identification of alkylation sites after sulfur mustard exposure
Transient receptor potential (TRP) channels are important in the sensing of pain and other stimuli. They may be triggered by electrophilic agonists...
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A single-cell massively parallel reporter assay detects cell-type-specific gene regulation
Massively parallel reporter gene assays are key tools in regulatory genomics but cannot be used to identify cell-type-specific regulatory elements...
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Expression of Large Full-Length PfEMP1 Proteins in HEK293 Cells
Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a family of proteins expressed on the surface of red blood cells infected by...
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Piezo1, the new actor in cell volume regulation
All animal cells control their volume through a complex set of mechanisms, both to counteract osmotic perturbations of the environment and to enable...
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Overexpression of CYP11A1 recovers cell cycle distribution in renal cell carcinoma Caki-1
BackgroundClear cell renal carcinoma is commonly known for its metastasis propensity to outspread to other organs and is asymptomatic in the early...
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Cell-Based hERG Channel Inhibition Assay in High-Throughput Format
Human ether-a-go-go-related gene (hERG) channel plays an essential role in the repolarization of the cardiac action potential. Genetic mutations and...
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Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System
Mammalian cell lines are one of the best options when it comes to the production of complex proteins requiring specific glycosylation patterns....
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Circular RNA-0007059 protects cell viability and reduces inflammation in a nephritis cell model by inhibiting microRNA-1278/SHP-1/STAT3 signaling
BackgroundIncreasing evidence has indicated that circular RNAs (circRNAs) play a role in various diseases. However, the influence of circRNAs in...
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Patch-clamp studies and cell viability assays suggest a distinct site for viroporin inhibitors on the E protein of SARS-CoV-2
BackgroundSARS-CoV-2 has caused a worldwide pandemic since December 2019 and the search for pharmaceutical targets against COVID-19 remains an...
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An Overview of the Cell Biology and Genetic Barriers to Virus Infections with Implications in Cell Line Development for Virotherapy
Viruses can be engineered for oncolysis, gene therapy, or vaccines. Oncolytic viruses and viral vector for cell and gene therapies are promising...
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Characterization of SARS-CoV-2 Glycoprotein Using a Quantitative Cell–Cell Fusion System
Coronaviruses (CoVs) infect host cells through the fusion of viral and cellular membrane and may also spread to the neighboring uninfected cells from...
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Nisin ZP, an Antimicrobial Peptide, Induces Cell Death and Inhibits Non-Small Cell Lung Cancer (NSCLC) Progression in vitro in 2D and 3D Cell Culture
Lung cancer is the leading cause of cancer deaths globally with most of the reported cases (> 85%) associated with non-small cell lung cancer...
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Alpha synuclein modulates mitochondrial Ca2+ uptake from ER during cell stimulation and under stress conditions
Alpha synuclein (a-syn) is an intrinsically disordered protein prevalent in neurons, and aggregated forms are associated with synucleinopathies...
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Culture of Continuous Cell Lines
This chapter describes the culture of various continuous cell lines. Continuous cell lines are generated either due to specific genetic or epigenetic...
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Cell Death-NO-Today: Effect of NO and RNS on Non-apoptotic Regulated Cell Death
Nitric oxide (NO), generated either enzymatically or non-enzymatically plays a significant role in cellular physiology. At low concentration, NO acts...
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Epithelial-to-mesenchymal transition and NF-kB pathways are promoted by a mutant form of DDB2, unable to bind PCNA, in UV-damaged human cells
BackgroundDNA-Damaged Binding protein 2 (DDB2) is a protein involved in the early step of Nucleotide Excision Repair. Recently, it has been reported...
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Upstream Processing of Viral Therapeutics: From Host Cell Expansion to Virus Production
Viral vectors have emerged as a promising approach to treat genetic diseases and cancers, marking the start of a new era in the pharmaceutical...
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Mammalian Cell Culture Types and Guidelines of Their Maintenance
Mammalian cell culture can be classified into three types, namely, primary, secondary, and continuous cultures. The maintenance and growth of cells...
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Cell Aggregation Assays for Homophilic Interactions Between Cell Surface Proteins
Many proteins expressed on the cellular surface provide signaling and cell adhesion properties required for vital cellular functions. These binding...