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Common Reagents and Medium for Mammalian Cell Culture
This chapter describes all common materials and reagents necessary for mammalian cell culture. Mammalian cells are normally present in tissues and...
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Isolation of total DNA from Placenta Samples, both Fresh and following Formalin and Paraffin Treatment
The isolation of DNA from placental tissue suspected of infection is an important tool for identifying microorganisms such as bacteria, fungi, and...
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Distribution of tetraspanins in bovine ovarian tissue and fresh/vitrified oocytes
Tetraspanin proteins are mostly known as organizers of molecular complexes on cell membranes, widely expressed on the surface of most nucleated...
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Targets preliminary screening for the fresh natural drug molecule based on Cosine-correlation and similarity-comparison of local network
BackgroundChinese herbal medicine is made up of hundreds of natural drug molecules and has played a major role in traditional Chinese medicine (TCM)...
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An improved medium for in vitro studies of female reproduction and oviposition in Schistosoma japonicum
BackgroundSchistosomiasis is a disease primarily caused by eggs laid by pathogens called schistosomes. Among the schistosome species infecting...
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The Complete Mitochondrial Genome of the Goitered Gazelle (Gazella subgutturosa hillieriana) from Ningxia Hui Autonomous Region, China
AbstractThe Mongolian subspecies of Goitred Gazelle ( Gazella subgutturosa hillieriana ) belongs to Artiodactyla and Bovidae. It is widely distributed...
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Transient pacing in pigs with complete heart block via myocardial injection of mRNA coding for the T-box transcription factor 18
The adenovirus-mediated somatic transfer of the embryonic T-box transcription factor 18 ( TBX18 ) gene can convert chamber cardiomyocytes into induced...
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Mycorrhiza-mediated recruitment of complete denitrifying Pseudomonas reduces N2O emissions from soil
BackgroundArbuscular mycorrhizal fungi (AMF) are key soil organisms and their extensive hyphae create a unique hyphosphere associated with microbes...
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Dental Pulp Stem Cell-Derived Conditioned Medium Alleviates Subarachnoid Hemorrhage-Induced Microcirculation Impairment by Promoting M2 Microglia Polarization and Reducing Astrocyte Swelling
Aneurysmal subarachnoid hemorrhage (SAH) can cause severe neurological deficits and high mortality. Early brain edema following SAH contributes to...
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Targeting the Akt/PI3K/mTOR signaling pathway for complete eradication of keloid disease by sunitinib
Keloid disease is a nodular lesion, tumor-like but not cancerous, and characterized of excessive proliferation of fibroblasts and deposition of...
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Embryonic Development of Pediculus humanus capitis: Morphological Update and Proposal of New External Markers for the Differentiation Between Early, Medium, and Late Eggs
Background and ObjectivesThe head louse Pediculus humanus capitis is a cosmopolitan ectoparasite that causes pediculosis. In the study of human...
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A single-cell transcriptomic atlas of complete insect nervous systems across multiple life stages
Molecular profiles of neurons influence neural development and function but bridging the gap between genes, circuits, and behavior has been very...
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Development and optimizing a simple and cost-effective medium for in vitro culture of Plasmodium berghei-ANKA strain with conserving its infectivity in BALB/c mice
ObjectivesThe current culture system for P. berghei still requires modifications in consistency and long-term maintenance of parasites considering...
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In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium
Tumor-associated macrophages (TAMs) are one of most important components of the tumor microenvironment. Although many assays have been developed to...
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Complete genome sequence of a novel bat mastadenovirus C strain isolated from Rhinolophus cornutus in Japan
Here, we report a novel bat adenovirus strain isolated from apparently healthy bats of the species Rhinolophus cornutus in Japan. The genome of the...
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Wnt/β-catenin pathway is a key signaling pathway to trastuzumab resistance in gastric cancer cells
BackgroundTrastuzumab is the only approved target agent for the first-line treatment of human epidermal growth factor receptor-2 (HER-2) positive...
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Comparison of individual and combination treatments with naproxen, prednisolone and hydroxychloroquine to treat Complete Freund’s Adjuvant induced arthritis
Aim of this study was to evaluate and compare the efficacy of anti-arthritic drugs (naproxen, prednisolone, and hydroxychloroquine) alone and in...
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A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors
Single-cell genomics is essential to chart tumor ecosystems. Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh...
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Adipocyte-conditioned medium induces resistance of breast cancer cells to lapatinib
BackgroundThe existence of a cross-talk between peritumoral adipocytes and cancer cells has been increasingly investigated. Several studies have...
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Complete genome analysis of the newly isolated Shigella sonnei phage vB_SsoM_Z31
This work describes the characterization and genome annotation of the newly isolated lytic phage vB_SsoM_Z31 (referred to as Z31), isolated from...
