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Transposon-Based Manufacturing of Human CAR-T Cells
In this chapter, the methodologies are outlined for generating CAR-T from PBMCs using transposon engineering. Additionally, some methods and guidance...
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Long noncoding RNAs emerge from transposon-derived antisense sequences and may contribute to infection stage-specific transposon regulation in a fungal phytopathogen
BackgroundThe genome of the obligate biotrophic phytopathogenic barley powdery mildew fungus Blumeria hordei is inflated due to highly abundant and...
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Slee** Beauty transposon mutagenesis in mouse intestinal organoids identifies genes involved in tumor progression and metastasis
To identify genes important for colorectal cancer (CRC) development and metastasis, we established a new metastatic mouse organoid model using Slee**...
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Themes and variations on piRNA-guided transposon control
PIWI-interacting RNAs (piRNAs) are responsible for preventing the movement of transposable elements in germ cells and protect the integrity of...
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Slee** Beauty transposon system for GDNF overexpression of entrapped stem cells in fibrin hydrogel in a rat model of Parkinson’s disease
There is currently no causal treatment available for Parkinson’s disease (PD). However, the use of glial cell line–derived neurotrophic factor (GDNF)...
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Generation of CAR-T Cells with Slee** Beauty Transposon Gene Transfer
Human T lymphocytes that transgenically express a chimeric antigen receptor (CAR)Chimeric antigen receptor (CAR) have proven efficacy and safety in...
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Teaching transposon classification as a means to crowd source the curation of repeat annotation – a tardigrade perspective
BackgroundThe advancement of sequencing technologies results in the rapid release of hundreds of new genome assemblies a year providing unprecedented...
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A decade of advances in transposon-insertion sequencing
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis...
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CRISPR/Cas9 technology: applications in oocytes and early embryos
CRISPR/Cas9, a highly versatile genome-editing tool, has garnered significant attention in recent years. Despite the unique characteristics of...
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Genomic approaches to trace the history of human brain evolution with an emerging opportunity for transposon profiling of ancient humans
Transposable elements (TEs) significantly contribute to sha** the diversity of the human genome, and lines of evidence suggest TEs as one of...
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Early Neurogenesis and Gliogenesis in Drosophila
In Drosophila, neurons and glial cells derive from neural precursor cells called neuroblasts glioblasts or neuroglioblasts. These neural precursor...
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A discrete ‘early-responder’ stromal-cell subtype orchestrates immunocyte recruitment to injured tissue
Following acute injury, stromal cells promote tissue regeneration by a diversity of mechanisms. Time-resolved single-cell RNA sequencing of muscle...
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Transposon-triggered innate immune response confers cancer resistance to the blind mole rat
Blind mole rats (BMRs) are small rodents, characterized by an exceptionally long lifespan (>21 years) and resistance to both spontaneous and induced...
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The Transposon Registry
Transposable elements in prokaryotes are found in many forms and therefore a robust nomenclature system is needed in order to allow researchers to...
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Generation of CAR+ T Lymphocytes Using the Slee** Beauty Transposon System
Adoptive immunotherapy of cancer using T cells expressing chimeric antigen receptors (CARs) is now an approved treatment for non-Hodgkin lymphoma...
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Inside the biology of early T-cell precursor acute lymphoblastic leukemia: the perfect trick
Early T-cell precursor acute lymphoblastic leukemia (ETP-ALL) is a rare, distinct subtype of T-ALL characterized by genomic instability, a dismal...
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Globally elevated levels of histone H3 lysine 9 trimethylation in early infancy are associated with poor growth trajectory in Bangladeshi children
BackgroundStunting is a global health problem affecting hundreds of millions of children worldwide and contributing to 45% of deaths in children...
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Genomic innovation and regulatory rewiring during evolution of the cotton genus Gossypium
Phenotypic diversity and evolutionary innovation ultimately trace to variation in genomic sequence and rewiring of regulatory networks. Here, we...
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De Novo Somatic Mosaicism of CYBB Caused by Intronic LINE-1 Element Insertion Resulting in Chronic Granulomatous Disease
Chronic granulomatosis disease (CGD) is a rare inborn error of immunity, characterized by phagocytic respiratory outbreak dysfunction. Mutations...
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Variable genome evolution in fungi after transposon-mediated amplification of a housekee** gene
BackgroundTransposable elements (TEs) can be key drivers of evolution, but the mechanisms and scope of how they impact gene and genome function are...
