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In Vitro Patch-Clamp
The patch-clamp technique is one of the most useful tools to analyze the function of electrically active cells such as neurons. This technique allows...
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Patch-Clamp Electrophysiological Analysis of Murine Melanopsin Neurons
Intrinsically photosensitive retinal ganglion cells (ipRGCs) regulate diverse aspects of mammalian physiology, including the synchronization of...
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Patch Clamp Technology in the Twenty-First Century
In the almost four decades since its inception, the patch clamp technique has transitioned from a specialist skill to a method commonly used among...
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Estimating the Ca2+ Permeability of NMDA Receptors with Whole-Cell Patch-Clamp Electrophysiology
In the mammalian central nervous system (CNS), fast excitatory transmission relies primarily on the ionic fluxes generated by ionotropic glutamate...
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Patch Clamp: The First Four Decades of a Technique That Revolutionized Electrophysiology and Beyond
Forty years ago, the introduction of a new electrophysiological technique, the patch clamp, revolutionized the fields of Cellular Physiology and...
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Advancing Ion Channel Research with Automated Patch Clamp (APC) Electrophysiology Platforms
Since its development on the cusp of the new millennium, automated patch clamp (APC) technology has matured over the last two decades. The increased...
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Single or Double Patch-Clamp Recordings In Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics
Patch-clamp recordings are the method of choice to define cell-type specific electrophysiological properties of single neurons and the synaptic...
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Dynamic Clamp on a Windows PC
Dynamic clamp is a powerful tool for interfacing computational models and real cells. We describe here how to set up and carry out dynamic clamp...
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Repetitive transcranial magnetic stimulation enhances the neuronal excitability of mice by regulating dynamic characteristics of Granule cells’ Ion channels
This study aims to explore the effects of acute high-frequency repetitive transcranial magnetic stimulation (hf-rTMS) on neuronal excitability of...
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Dynamic and selective engrams emerge with memory consolidation
Episodic memories are encoded by experience-activated neuronal ensembles that remain necessary and sufficient for recall. However, the temporal...
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Single-compartment model of a pyramidal neuron, fitted to recordings with current and conductance injection
For single neuron models, reproducing characteristics of neuronal activity such as the firing rate, amplitude of spikes, and threshold potentials as...
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HCN-Channel-Dependent Hyperexcitability of the Layer V Pyramidal Neurons in IL-mPFC Contributes to Fentanyl-Induced Hyperalgesia in Male Rats
Opioids are often first-line analgesics in pain therapy. However, prolonged use of opioids causes paradoxical pain, termed “opioid-induced...
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Dynamic physiological α-synuclein S129 phosphorylation is driven by neuronal activity
In Parkinson’s disease and other synucleinopathies, the elevation of α-synuclein phosphorylated at Serine129 (pS129) is a widely cited marker of...
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The Plant Vacuole as Heterologous System to Characterize the Functional Properties of TPC Channels
Human TPC channels are an emerging family of intracellular proteins fundamental for cell physiology and involved in various severe pathologies. Their...
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Neuronal differentiation and functional maturation of neurons from neural stem cells induced by bFGF-chitosan controlled release system
Available methods for differentiating stem cells into neurons require a large number of cytokines and neurotrophic factors, with complex steps and...
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Electrophysiology of Dendritic Spines: Information Processing, Dynamic Compartmentalization, and Synaptic Plasticity
For many years, synaptic transmission was considered as information transfer between presynaptic neuron and postsynaptic cell. At the synaptic level,...
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Loss of the large conductance calcium-activated potassium channel causes an increase in mitochondrial reactive oxygen species in glioblastoma cells
Mitochondrial potassium (mitoK) channels play an important role in cellular physiology. These channels are expressed in healthy tissues and cancer...
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Cellular electrophysiological effects of botulinum toxin A on neonatal rat cardiomyocytes and on cardiomyocytes derived from human-induced pluripotent stem cells
Botulinum toxin A is a well-known neurotransmitter inhibitor with a wide range of applications in modern medicine. Recently, botulinum toxin A...
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Sexual differences in locus coeruleus neurons and related behavior in C57BL/6J mice
BackgroundIn addition to social and cultural factors, sex differences in the central nervous system have a critical influence on behavior, although...
