Search
Search Results
-
Textbook on Cloning, Expression and Purification of Recombinant Proteins
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA...
-
Selection of Cloning and Expression Plasmid Vectors
Purification of proteins using recombinant DNA technology is crucial for studying its structure, function, and interactions with other...
-
TOPO Cloning for Efficient Plasmid Construction
This chapter outlines the use of TOPO cloning for streamlined generation of a recombinant plasmid containing your gene of interest for use in the...
-
A High-Throughput Strategy for T-Cell Receptor Cloning and Expression
Expression of T-cell receptor (TCR) genes is a critical step for TCR characterization and epitope identification. The recent interest in using...
-
Cloning and Gene Manipulation
Gene cloning has continually been one of the many important discoveries in the areas of molecular and cell biology. The advent of recombinant DNA...
-
Cloning and Expression Analysis of Onion (Allium cepa L.) MADS-Box Genes and Regulation Mechanism of Cytoplasmic Male Sterility
Flower organ development is one of the most important processes in plant life. However, onion CMS (cytoplasmic male sterility) shows an abnormal...
-
Molecular Cloning, Characterization and Expression Pattern of Two RARs and a RAR-RXRγ Genes in Carassius auratus
AbstractAs members of nuclear hormone receptor superfamily, retinoic acid receptor and retinoid X receptors are involved in the transduction of...
-
Heterologous Expression of Candida Antifungal Target Genes in the Model Organism Saccharomyces cerevisiae
Understanding how a gene variant may influence antifungal resistance, or other phenotypic characteristics, is an important step in determining or...
-
Transformation and Protein Expression
Transformation is an important step in recombinant DNA technology that allows transfer of genetic material inside cells. Often, this transfer...
-
Expression of Dystrophin Dp71 Splice Variants Is Temporally Regulated During Rodent Brain Development
Dystrophin Dp71 is the major product of the Duchenne muscular dystrophy ( DMD ) gene in the brain, and its loss in DMD patients and mouse models leads...
-
Analysis of Surface Expression of NMDAR Subunits in Primary Hippocampal Neurons
The expression and activity of ionotropic glutamate receptors control signal transduction at the excitatory synapses in the CNS. The NMDAR comprises...
-
Nonviral Platform for Expression of Recombinant Protein in Insect Cells
Nonviral transfection has been used to express various recombinant proteins, therapeutics, and virus-like particles (VLP) in mammalian and insect...
-
A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses
The baculovirus expression vector system (BEVS) is recognized as a powerful platform for producing challenging proteins and multiprotein complexes...
-
Cloning and expression studies on glutathione S-transferase like-gene in honey bee for its role in oxidative stress
Glutathione S-transferases (GSTs) constitute an important multifunctional enzyme family that plays vital roles in cellular detoxification and...
-
Expression of dengue virus and Zika virus NS2B-NS3pro constructs alter cellular fatty acids, but co-expression with a Zika virus virus-like particle is detrimental to virus-like particle expression
ObjectiveStudies have shown that Flavivirus infection remodels the host cell to favour viral replication. In particular, the host cell lipid profile...
-
Identification of a Tetrahymena species infecting guppies, pathology, and expression of beta-tubulin during infection
Tetrahymenosis is caused by the ciliated protozoan Tetrahymena and is responsible for serious economic losses to the aquaculture industry worldwide....
-
Expression of Single-Domain Soluble and Disulfide-Folded PfEMP1 Antigens in the Escherichia coli SHuffle Expression System
The genome of Plasmodium falciparum has an A/T content of around 81%. This, together with a high cysteine content and the high molecular weight of...
-
Rapid cloning of antigen-specific T-cell receptors by leveraging the cis activation of T cells
It is commonly understood that T cells are activated via trans interactions between antigen-specific T-cell receptors (TCRs) and antigenic peptides...
-
Stability and integrity of self-assembled bovine parvovirus virus‑like particles (BPV‑VLPs) of VP2 and combination of VP1VP2 assisted by baculovirus-insect cell expression: a potential logistical platform for vaccine deployment
BackgroundBovine parvovirus (BPV) is an autonomous DNA virus with a smaller molecular size and subtle differences in its structural proteins, unlike...
-
Cloning and Detection of Aptamer-Ribozyme Conjugations
RNA aptamers can be used to target proteins or nucleic acids for therapeutic purposes and are candidates for RNA-mediated gene therapy. Like other...