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Reverse Genetics of Zika Virus Using a Bacterial Artificial Chromosome
Zika virus (ZIKV) is a mosquito-borne member of the Flaviviridae family that has become a global threat to human health. Although ZIKV has been known...
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Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a new member of the Coronaviridae family responsible for the coronavirus disease 19...
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Ring Chromosome 15
Ring chromosome 15 (RC15) can be detected using cell-based karyoty** and fluorescence in situ hybridization (FISH) test to determine its structure...
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Ring Chromosome 4
We describe the cytogenomic and clinical characteristics of 47 individuals of ring chromosome 4 (RC4) published in the literature. In nine cases,...
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Ring Chromosome 19
Ring chromosome 19 (RC19) is a rare genetic abnormality with variable clinical manifestations, ranging from normalcy to developmental and...
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Simultaneous measurement of the size and methylation of chromosome 4qA-D4Z4 repeats in facioscapulohumeral muscular dystrophy by long-read sequencing
BackgroundFacioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant muscular disorder characterized by asymmetric muscle wasting and...
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Molecular Mechanisms of Ring Chromosome Formation and Instability
Ring chromosome (RC) formation is a result of “rescue” chromosomes with DNA double-strand breaks. The mechanisms of formation of RCs are not limited...
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Sequencing-based analysis of microbiomes
Microbiomes occupy a range of niches and, in addition to having diverse compositions, they have varied functional roles that have an impact on...
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Gauge your phage: benchmarking of bacteriophage identification tools in metagenomic sequencing data
BackgroundThe prediction of bacteriophage sequences in metagenomic datasets has become a topic of considerable interest, leading to the development...
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Beyond assembly: the increasing flexibility of single-molecule sequencing technology
The maturation of high-throughput short-read sequencing technology over the past two decades has shaped the way genomes are studied. Recently,...
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Methylation status of genes esca** from X-chromosome inactivation in patients with X-chromosome rearrangements
BackgroundX-chromosome inactivation (XCI) is a mechanism in which one of two X chromosomes in females is randomly inactivated in order to compensate...
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Genome Sequencing
In this chapter, you will learn how to read genomic DNA with Sanger method and Next-Generation Sequencing techniques. We will cover natural as well...
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Highly Efficient Base Editing in Viral Genome Based on Bacterial Artificial Chromosome Using a Cas9-Cytidine Deaminase Fused Protein
Viruses evolve rapidly and continuously threaten animal health and economy, posing a great demand for rapid and efficient genome editing technologies...
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16p13.11p11.2 triplication syndrome: a new recognizable genomic disorder characterized by optical genome map** and whole genome sequencing
Highly identical segmental duplications (SDs) account for over 5% of the human genome and are enriched in the short arm of the chromosome 16. These...
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Integration of CRISPR/Cas9 with artificial intelligence for improved cancer therapeutics
Gene editing has great potential in treating diseases caused by well-characterized molecular alterations. The introduction of clustered regularly...
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Revelations in Thymic Epithelial Cell Biology and Heterogeneity from Single-Cell RNA Sequencing and Lineage Tracing Methodologies
Thymic epithelial cells (TECs) make up the thymic microenvironments that support the generation of a functionally competent and self-tolerant T-cell...
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Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep
BackgroundThe integration of molecular data from hosts, parasites, and microbiota can enhance our understanding of the complex biological...
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Towards population-scale long-read sequencing
Long-read sequencing technologies have now reached a level of accuracy and yield that allows their application to variant detection at a scale of...
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Establishment and partial characterisation of a new cell line derived from adult tissues of the tsetse fly Glossina morsitans morsitans
BackgroundInsect cell lines play a vital role in many aspects of research on disease vectors and agricultural pests. The tsetse fly Glossina...
