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Inhibitory Potential of Carnosine and Aminoguanidine Towards Glycation and Fibrillation of Albumin: In-vitro and Simulation Studies
Carnosine is beta-alanyl histidine, a dipeptide, endogenously produced in our body by the carnosine synthase enzyme. It is an antioxidant, thus...
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Effect of (R)-2-Amino-6-(1R,2S)-1,2-Dihydroxypropyl)-5,6,7,8-Tetrahydropterin-4(3H)-One and Its Structural Analogues on the Temperature Stability of Tryptophan Hydroxylase 2 with the P447R Mutation
The enzyme tryptophan hydroxylase 2 (TPH2) catalyzes the hydroxylation of L-tryptophan to L-5-hydroxytryptophan (5-HTP), the first and the key step...
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Effect of pH on the Conformational Transition of Silk Fibroin in Aqueous Solution Monitored by Thioflavin-T Fluorescence
In this work, the potential application of the fluorescence dye Thioflavin-T (ThT), which can specifically bind to amyloid, as a powerful tool for...
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Biophysical Methods for Characterization of Antibody-Drug Conjugates
Antibody-drug conjugates (ADC) are made up of three components: (1) a mAb specific to cells of choice, (2) a small molecule with desired end goal,...
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Stimuli-Responsive Insulin Delivery Devices
The development of new diabetes treatment strategies has garnered much interest given that conventional management therapies for type 1 diabetes fail...
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Investigating the chaperone activity of β-casein in preventing amyloid formation in κ-casein in the presence of dextran
In vitro studies into proteins which are prone to form amyloid are very important. Today, researchers are trying to understand the exact mechanism of...
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Injectable Supramolecular Hydrogel from Insulin-Loaded Triblock PCL-PEG-PCL Copolymer and γ-Cyclodextrin with Sustained-Release Property
Supramolecular hydrogels formed by cyclodextrins and polymers have been widely investigated as a biocompatible, biodegradable and controllable drug...
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Chaperone Potential of Pulicaria undulata Extract in Preventing Aggregation of Stressed Proteins
This study examined the effect of an aqueous extract of Pulicaria undulata on the 1,4-dithiothreitol (DTT)-induced aggregation of proteins. The...
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Assessment of Physical Stability of an Antibody Drug Conjugate by Higher Order Structure Analysis: Impact of Thiol- Maleimide Chemistry
PurposeTo provide a systematic biophysical approach towards a better understanding of impact of conjugation chemistry on higher order structure and...
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High-Throughput Biophysical Approaches to Therapeutic Protein Development
High-throughput methods have become indispensible tools in the development of proteins as therapeutic agents and are being applied in every phase of...
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Methods for the Investigation of Thyroid Function
In the developed world, all routine clinical laboratories should be able to perform tests for the assessment of the pituitary–thyroid axis. Testing...
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Beta-amyloid toxicity increases with hydrophobicity in the presence of metal ions
Alzheimer’s disease is a multifactorial neurodegenerative disorder characterized by the pathological brain deposition of neurofibrillary tangles and...
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Conformational Transitions of the Catalytic Domain of Heme-Regulated Eukaryotic Initiation Factor 2α Kinase, a Key Translational Regulatory Molecule
In mammalian cells, the heme-regulated inhibitor (HRI) plays a critical role in the regulation of protein synthesis at the initiation step through...
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Conformational Transitions in Ariesaema curvatum Lectin: Characterization of an Acid Induced Active Molten Globule
Biophysical characterization of a lectin from Ariesaema curvatum (ACL) was carried out using steady state as well as time resolved fluorescence and...
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Synthesis and Spectroscopy of Novel Branched Fluorescent Dyes Containing Benzophenone Parts and the Possibility as Fluorescence Probes
This paper describes a new fluorescent family of branched dyes containing benzophenone unit including 4 -N, N-diphenylamino- 4 ′-phenacyl-stilbene ( C1 ), 4,4...
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Steady State and Time-Resolved Fluorescence Studies of a Hemagglutinin from Moringa oleifera
The saccharide binding and conformational characterization of a hemagglutinin, a low molecular weight protein from the seeds of Moringa oleifera was...
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Biological polyamines inhibit nucleic-acid-induced polymerisation of prion protein
Nucleic-acid-induced polymerisation of prion protein, when monitored by anilino naphthalene sulfonic acid dye, shows, successively, an immediate...
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Human Lysozyme
Lysozyme is perhaps the protein whose structure, stability, and folding behavior has been studied most widely over many years to define general...
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Dendrimer Interactions with Hydrophobic Fluorescent Probes and Human Serum Albumin
The interactions between three types of polyamidoamine dendrimers (with anionic, cationic, and neutral charge on a surface) and fluorescent dye...
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Protein-Based Biosensors for Diabetic Patients
In this article we show the recent progress in the field of glucose sensing based on the utilization of enzymes and proteins as probes for stable and...